Healing of Fresh Frozen Bone Allograft with or without Platelet-Rich Plasma: A Histologic and Histometric Study in Rats

The purpose of this study was to radiographically analyze the effect of autologous platelet rich plasma (PRP) on the healing of fresh frozen bone allograft (FFBA) placed in surgically created resection defects in mandibles of dogs. Bilateral resection defects measuring 1.5 cm × 1 cm were surgically created on the inferior border of the mandible in 10 adult male dogs. The defects were randomly divided into three groups: C, FFBA, and FFBA/PRP. In Group C, the defect was filled by blood clot only. In Group FFBA, the defect was filled with particulate fresh frozen bone allograft. In Group FFBA/PRP, it was filled with particulate fresh frozen bone allograft combined with PRP. At 90 days postoperative, standardized radiographs of the mandibles were obtained and results were quantitatively evaluated. Analysis of digitized radiographs indicated that non-PRP grafts were significantly less dense than the PRP grafts. Group FFBA/PRP also presented a statistically greater mineralized tissue area than Groups C and FFBA. Within the limits of this study, it can be concluded that PRP enhanced the healing of FFBA in resection defects in mandibles of dogs.

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Effect of platelet-rich plasma on bone healing of fresh frozen bone allograft in mandibular defects: a histomorphometric study in dogs

Clinical Oral Implants Research ◽

10.1111/clr.12008 ◽

2012 ◽

Vol 24 (12) ◽

pp. 1347-1353 ◽

Cited By ~ 7

Author(s):

Michel R. Messora ◽

Maria J. H. Nagata ◽

Natália M. Pola ◽

Natália de Campos ◽

Stephen E. Fucini ◽

...

Keyword(s):

Bone Healing ◽

Platelet Rich Plasma ◽

Bone Allograft ◽

Histomorphometric Study ◽

Fresh Frozen ◽

Frozen Bone

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Platelet rich plasma and fresh frozen bone allograft as enhancement of implant fixation an experimental study in dogs

Journal of Orthopaedic Research® ◽

10.1016/j.orthres.2003.10.006 ◽

2004 ◽

Vol 22 (3) ◽

pp. 653-658 ◽

Cited By ~ 84

Author(s):

T. B. Jensen ◽

O Rahbek ◽

S. Overgaard ◽

K. Søballe

Keyword(s):

Experimental Study ◽

Platelet Rich Plasma ◽

Bone Allograft ◽

Implant Fixation ◽

Fresh Frozen ◽

Frozen Bone

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Mandibular Reconstruction Using Fresh Frozen Bone Allograft After Conservative Enucleation of a Mandibular Odontogenic Myxoma

Journal of Craniofacial Surgery ◽

10.1097/scs.0b013e31824dbff6 ◽

2012 ◽

Vol 23 (3) ◽

pp. 831-835 ◽

Cited By ~ 7

Author(s):

Massimo Albanese ◽

Pier Francesco Nocini ◽

Andrea Fior ◽

Alberto Rizzato ◽

Maria Giulia Cristofaro ◽

...

Keyword(s):

Bone Allograft ◽

Mandibular Reconstruction ◽

Odontogenic Myxoma ◽

Fresh Frozen ◽

Frozen Bone

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Clinical, Histological and Cellular Evaluation of Vertico-Lateral Maxillary Reconstruction Associating Alveolar Osteogenic Distraction and Fresh-Frozen Bone Allograft

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-13-00102 ◽

2015 ◽

Vol 41 (3) ◽

pp. 326-331 ◽

Cited By ~ 2

Author(s):

Emanuela Prado Ferraz ◽

Adalberto Luiz Rosa ◽

Paulo Tambasco de Oliveira ◽

Thiago Santana Santos ◽

Danilo Maeda Reino ◽

...

Keyword(s):

Bone Allograft ◽

Fresh Frozen ◽

Frozen Bone

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Fresh-Frozen Bone Allografts in Maxillary Ridge Augmentation: Histologic Analysis

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-09-00108 ◽

2011 ◽

Vol 37 (2) ◽

pp. 223-231 ◽

Cited By ~ 35

Author(s):

Cintia Mussi Milani Contar ◽

João Rodrigo Sarot ◽

Maite Barroso da Costa ◽

Jayme Bordini ◽

Antonio Adilson Soares de Lima ◽

...

Keyword(s):

Bone Allograft ◽

Autogenous Bone ◽

Control Group ◽

Allogeneic Bone ◽

Operative Trauma ◽

Ridge Augmentation ◽

Significant Difference ◽

Fresh Frozen ◽

And Control ◽

Frozen Bone

Abstract Bone allograft has become an alternative to autogenous bone due to its decreased operative trauma and the almost unlimited supply of reconstructive material. The aim of the present study was to histologically evaluate the suitability of fresh-frozen bone graft (test group) used in maxillary ridge augmentation, comparing it to autogenous bone (native maxilla: control group). During the re-entry procedures, 9 months after the fresh-frozen allogeneic bone blocks were placed in the atrophic maxillary ridges, bone cores were removed with a trephine bur from test and control treatments in the same patient. Routine histologic processing using hematoxylin and eosin and Picrosirius staining was performed. Mature and immature collagen area and density analysis were carried out for both groups under polarization. The results of Student’s t test for paired samples (P > .05) showed no statistically significant difference in mature and immature collagen area or density percentage between test and control groups. Histologically similar bone formation patterns were observed in both groups. We concluded that fresh-frozen bone allograft is a biologically acceptable alternative for augmentation of the deficient alveolar ridge, showing a similar collagen pattern to that of autogenous bone.

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