Uptake and release of adenosine in isolated rat fat cells

1979 ◽  
Vol 105 (3) ◽  
pp. 257-267 ◽  
Author(s):  
BERTIL B. FREDHOLM ◽  
PAUL HJEMDAHL
Keyword(s):  
Fat Cells ◽  
Rat Fat Cells ◽  
Uptake And Release ◽  
Isolated Rat

Lipoprotein lipase secretion from isolated rat fat cells of different size

Life Sciences ◽  
1986 ◽  
Vol 39 (22) ◽  
pp. 2111-2119 ◽  
Author(s):  
Susan K. Fried ◽  
Mario DiGirolamo
Keyword(s):  
Lipoprotein Lipase ◽  
Fat Cells ◽  
Rat Fat Cells ◽  
Isolated Rat

scholarly journals A rapid immunological procedure for the isolation of hormonally sensitive rat fat-cell plasma membrane

1976 ◽  
Vol 154 (1) ◽  
pp. 11-21 ◽  
Author(s):  
J P Luzio ◽  
A C Newby ◽  
C N Hales
Keyword(s):  
Plasma Membrane ◽  
Adenylate Cyclase ◽  
Plasma Membranes ◽  
Membrane Preparation ◽  
Fat Cells ◽  
Fat Cell ◽  
Cell Plasma ◽  
Rat Fat Cells ◽  
Plasma Membrane Preparation ◽  
Isolated Rat

1. A rapid method for the isolation of hormonally sensitive rat fat-cell plasma membranes was developed by using immunological techniques. 2. Rabbit anti-(rat erythrocyte) sera were raised and shown to cross-react with isolated rat fat-cells. 3. Isolated rat fat-cells were coated with rabbit anti-(rat erythrocyte) antibodies, homogenized and the homogenate made to react with an immunoadsorbent prepared by covalently coupling donkey anti-(rabbit globulin) antibodies to aminocellulose. Uptake of plasma membrane on to the immunoadsorbent was monitored by assaying the enzymes adenylate cyclase and 5′-nucleotidase and an immunological marker consisting of a 125I-labelled anti-(immunoglobulin G)-anti-cell antibody complex bound to the cells before fractionation. Contamination of the plasma-membrane preparation by other subcellular fractions was also investigated. 4. By using this technique, a method was developed allowing 25-40% recovery of plasma membrane from fat-cell homogenates within 30 min of homogenization. 5. Adenylate cyclase in the isolated plasma-membrane preparation was stimulated by 5 μm-adrenaline.

A significant increase of lysophosphatidylinositol 4-phosphate with insulin in isolated rat fat cells

FEBS Letters ◽  
1987 ◽  
Vol 224 (1) ◽  
pp. 137-141 ◽  
Author(s):  
Yoshihiko Kuroda ◽  
Hidetaka Nakayama ◽  
Teruo Ishibashi ◽  
Shin Aoki ◽  
Satoshi Tushima ◽  
...  
Keyword(s):  
Fat Cells ◽  
Rat Fat Cells ◽  
Isolated Rat

scholarly journals Inhibition of the Action of Nonsuppressible Insulin-Like Activity on Isolated Rat Fat Cells by Binding to its Carrier Protein

1979 ◽  
Vol 63 (5) ◽  
pp. 1077-1084 ◽  
Author(s):  
J. Zapf ◽  
E. Schoenle ◽  
G. Jagars ◽  
I. Sand ◽  
J. Grunwald ◽  
...  
Keyword(s):  
Carrier Protein ◽  
Fat Cells ◽  
Rat Fat Cells ◽  
Isolated Rat ◽  
Insulin Like Activity
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