Biochemical and Molecular Genetic Aspects of Pyruvate Dehydrogenase Complex from Saccharomyces cerevisiae

1989 ◽  
Vol 573 (1 Alpha-Keto Ac) ◽  
pp. 155-167 ◽  
Author(s):  
LESTER J. REED ◽  
KAREN S. BROWNING ◽  
XIAO-DA NIU ◽  
ROBERT H. BEHAL ◽  
DAVID J. UHLINGER
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pyruvate Dehydrogenase ◽  
Pyruvate Dehydrogenase Complex ◽  
Molecular Genetic ◽  
Dehydrogenase Complex

On The Unique Structural Organization of the Saccharomyces Cerevisiae Pyruvate Dehydrogenase Complex

1998 ◽  
Vol 4 (S2) ◽  
pp. 954-955
Author(s):  
James K. Stoops ◽  
Z. Hong Zhou ◽  
John P. Schroeter ◽  
Steven J. Kolodziej ◽  
R. Holland Cheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Saccharomyces Cerevisiae ◽  
Pyruvate Dehydrogenase ◽  
Structural Organization ◽  
Pyruvate Dehydrogenase Complex ◽  
The Other ◽  
Oxidative Decarboxylation ◽  
Multienzyme Complex ◽  
The Core ◽  
Dehydrogenase Complex

Dihydrohpoamide acetyl transferase (E2), a catalytic and structural component of a multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate, forms the central core to which the other components are bound. We have utilized protein engineering and 3-D electron microscopy to study the structural organization of the largest multienzyme complex known (Mr ∼ 107). The structures of the truncated 60-mer core (tE2) and complexes of the tE2 associated with a binding protein (BP), and the BP associated with its dihydrohpoamide dehydrogenase (BP'E3) and the intact E2 associated with BP and the pyruvate dehydrogenase (E1) were determined (Figs. 1 and 2). The tE2 core is a pentagonal dodecahedron consisting of 20 cone-shaped trimers interconnected by 30 bridges.Previous studies have given rise to the generally accepted belief that BP and BP'E3 components are bound on the outside of the E2 scaffold and that E1 is similarly bound to the core in variable positions by flexible tethers.

scholarly journals The pyruvate dehydrogenase complex of Saccharomyces cerevisiae is regulated by phosphorylation

FEBS Letters ◽  
1995 ◽  
Vol 373 (2) ◽  
pp. 111-114 ◽  
Author(s):  
A.Gordon James ◽  
Ritchard M. Cook ◽  
Shauna M. West ◽  
J.Gordon Lindsay
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pyruvate Dehydrogenase ◽  
Pyruvate Dehydrogenase Complex ◽  
Dehydrogenase Complex
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