Ratiometric Analysis of Fluorescence Lifetime for Probing Binding Sites in Albumin with Near-Infrared Fluorescent Molecular Probes

2007 ◽  
Vol 83 (6) ◽  
pp. 1371-1378 ◽  
Author(s):  
Mikhail Y. Berezin ◽  
Hyeran Lee ◽  
Walter Akers ◽  
Gregory Nikiforovich ◽  
Samuel Achilefu
Keyword(s):  
Fluorescence Lifetime ◽  
Binding Sites ◽  
Near Infrared ◽  
Molecular Probes ◽  
Fluorescent Molecular Probes

scholarly journals Near-infrared fluorescent molecular probes for imaging and diagnosis of nephro-urological diseases

2021 ◽  
Author(s):  
Jiaguo Huang ◽  
Kanyi Pu
Keyword(s):  
Near Infrared ◽  
Molecular Probes ◽  
Imaging Depth ◽  
Fluorescent Molecular Probes ◽  
Urological Diseases

Near-infrared fluorescent molecular probes with improved imaging depth and optimized biodistribution have been reviewed, showing great potential for diagnosis of nephro-urological diseases.

scholarly journals In Vivo Resolution of Multiexponential Decays of Multiple Near-Infrared Molecular Probes by Fluorescence Lifetime-Gated Whole-Body Time-Resolved Diffuse Optical Imaging

2007 ◽  
Vol 6 (4) ◽  
pp. 7290.2007.00020 ◽  
Author(s):  
Walter Akers ◽  
Frederic Lesage ◽  
Dewey Holten ◽  
Samuel Achilefu
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Near Infrared ◽  
Molecular Probes ◽  
Whole Body ◽  
Diffuse Optical Imaging ◽  
Fluorescence Lifetimes ◽  
Time Resolved

The biodistribution of two near-infrared fluorescent agents was assessed in vivo by time-resolved diffuse optical imaging. Bacteriochlorophyll a (BC) and cypate-glysine-arginine-aspartic acid-serine-proline-lysine-OH (Cyp-GRD) were administered separately or combined to mice with subcutaneous xenografts of human breast adenocarcinoma and slow-release estradiol pellets for improved tumor growth. The same excitation (780 nm) and emission (830 nm) wavelengths were used to image the distinct fluorescence lifetime distribution of the fluorescent molecular probes in the mouse cancer model. Fluorescence intensity and lifetime maps were reconstructed after raster-scanning whole-body regions of interest by time-correlated single-photon counting. Each captured temporal point-spread function (TPSF) was deconvolved using both a single and a multiexponental decay model to best determine the measured fluorescence lifetimes. The relative signal from each fluorophore was estimated for any region of interest included in the scanned area. Deconvolution of the individual TPSFs from whole-body fluorescence intensity scans provided corresponding lifetime images for comparing individual component biodistribution. In vivo fluorescence lifetimes were determined to be 0.8 ns (Cyp-GRD) and 2 ns (BC). This study demonstrates that the relative biodistribution of individual fluorophores with similar spectral characteristics can be compartmentalized by using the time-domain fluorescence lifetime gating method.

scholarly journals Long Fluorescence Lifetime Molecular Probes Based on Near Infrared Pyrrolopyrrole Cyanine Fluorophores for In Vivo Imaging

2009 ◽  
Vol 97 (9) ◽  
pp. L22-L24 ◽  
Author(s):  
Mikhail Y. Berezin ◽  
Walter J. Akers ◽  
Kevin Guo ◽  
Georg M. Fischer ◽  
Ewald Daltrozzo ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Molecular Probes

scholarly journals Non-Covalently Pre-Assembled High-Performance Near-Infrared Fluorescent Molecular Probes for Cancer Imaging

2018 ◽  
Vol 24 (52) ◽  
pp. 13821-13829 ◽  
Author(s):  
Scott K. Shaw ◽  
Wenqi Liu ◽  
César Fernando Azael Gómez Durán ◽  
Cynthia L. Schreiber ◽  
María de Lourdes Betancourt Mendiola ◽  
...  
Keyword(s):  
High Performance ◽  
Near Infrared ◽  
Cancer Imaging ◽  
Molecular Probes ◽  
Fluorescent Molecular Probes

Fluorescent Molecular Probes II. The Synthesis, Spectral Properties and Use of Fluorescent Solvatochromic Dapoxyl Dyes

1997 ◽  
Vol 66 (4) ◽  
pp. 424-431 ◽  
Author(s):  
Zhenjun Diwu ◽  
Yixin Lu ◽  
Cailan Zhang ◽  
Dieter H. Klaubert ◽  
Richard P. Haugland
Keyword(s):  
Spectral Properties ◽  
Molecular Probes ◽  
Fluorescent Molecular Probes

Anti-Scarring Drug Screening with Near-Infrared Molecular Probes Targeting Fibroblast Activation Protein-α

2018 ◽  
Vol 1 (6) ◽  
pp. 2054-2061 ◽  
Author(s):  
David C. Yeo ◽  
Christian Wiraja ◽  
Qingqing Miao ◽  
Xiaoyu Ning ◽  
Kanyi Pu ◽  
...  
Keyword(s):  
Drug Screening ◽  
Near Infrared ◽  
Fibroblast Activation Protein ◽  
Molecular Probes ◽  
Fibroblast Activation

scholarly journals Perfusion-based fluorescence imaging method delineates diverse organs and identifies multifocal tumors using generic near-infrared molecular probes

2018 ◽  
Vol 11 (4) ◽  
pp. e201700232 ◽  
Author(s):  
Jessica Miller ◽  
Steven T. Wang ◽  
Inema Orukari ◽  
Julie Prior ◽  
Gail Sudlow ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Molecular Probes ◽  
Imaging Method
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