scholarly journals Optimising the method for isolating ocular surface microRNA using impression cytology

2022 ◽  
Vol 100 (S267) ◽  
Author(s):  
Emily Greenan ◽  
Joan Ní Gabhann‐Dromgoole ◽  
Conor Murphy
Keyword(s):  
Ocular Surface ◽  
Impression Cytology

Simplified ex-vivo drug evaluation in ocular surface cells: Culture on cellulose filters of cells obtained by impression cytology

2021 ◽  
pp. 108827
Author(s):  
Adam Master ◽  
Wei Huang ◽  
Liqun Huang ◽  
Wenyi Li ◽  
Sait Saglam ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Ex Vivo ◽  
Drug Evaluation ◽  
Impression Cytology

scholarly journals Role of impression cytology in ocular surface disorders

2020 ◽  
Vol 6 (1) ◽  
pp. 42-47
Author(s):  
Rupali Maheshgauri ◽  
Aishwarya Thakre ◽  
Manvi Aggarwal ◽  
Priyanka Mantri ◽  
Asim Naik
Keyword(s):  
Ocular Surface ◽  
Impression Cytology

Ocular Surface Impression Cytology

2003 ◽  
Vol 10 (6) ◽  
pp. 328-337 ◽  
Author(s):  
Penelope McKelvie
Keyword(s):  
Ocular Surface ◽  
Impression Cytology

An impression cytology based study of ocular surface in an urban population

2012 ◽  
Vol 33 (2) ◽  
pp. 153-157
Author(s):  
Somnath Mukhopadhyay ◽  
Jayanta Dutta ◽  
Jayati Mitra ◽  
Ratnesh Prakash ◽  
Himadri Datta
Keyword(s):  
Ocular Surface ◽  
Urban Population ◽  
Impression Cytology

scholarly journals CImP: Cellular Imprinting Proteomics applied to ocular disorders elicited by Congenital Zika virus Syndrome

2019 ◽  
Author(s):  
Livia Rosa-Fernandes ◽  
Raquel Hora Barbosa ◽  
Maria Luiza B. dos Santos ◽  
Claudia B. Angeli ◽  
Thiago P. Silva ◽  
...  
Keyword(s):  
Zika Virus ◽  
Ocular Surface ◽  
Molecular Mechanisms ◽  
Membrane Filter ◽  
Neurological Diseases ◽  
Specific Protein ◽  
Protein Markers ◽  
Impression Cytology ◽  
Zikv Infection ◽  
Molecular Alterations

AbstractIMPORTANCEOcular complications in infants with Congenital Zika Syndrome (CZS) have been reported. However, the molecular mechanisms underlying of eye dysfunctions are presently unknown.OBJECTIVEA method (termed Cellular Imprinting Proteomics, CImP) for the identification and quantification of the ocular surface proteome using a minimally invasive membrane filter device is described. Moreover, The CImP method was applied to profile the molecular alterations in the eyes of infants exposed to Zika virus (ZIKV) infection during gestation.DESIGN, SETTINGS AND PARTICIMPANTSThe CImP method was applied to a cohort divided into three conditions: 1) Ctrl (infants with no infectious diseases, n=5). 2) Zikv (infants exposed to ZIKV gestation, with no microcephaly, n=5). 3) ZikvCZS(infants exposed to ZIKV, with microcephaly, n=3). All conditions were age and sex-matched. An improved impression cytology method was used to capture the outermost ocular surface cells. The number of impression cytology membrane collected was: Ctrl (12), Zikv (14) and ZikvCZS(8). Proteins were extracted and analysed using mass spectrometry-based proteomics technology followed by statistical analysis. Parallel reaction monitoring was performed to validate the expression of specific protein markers.RESULTSUsing the CImP method, 2209 proteins were identified on the membrane-captured conjunctiva epithelial cells. Modulation of neutrophil degranulation, cell death, ocular and neurodevelopment pathways are reported in infants with CZS compared to matched controls. Moreover, the molecular pattern of ocular surface cells retrieved from infants infected during the gestation but with no CZS was different from matched controls.CONCLUSIONS AND PERSPECTIVESMolecular alterations in the ocular cell surface associated to ZIKV infection with and without CZS complications are reported for the first time. We predict that this method will be introduced successfully in the study of several neurological diseases with the aim to identify novel diagnostic and therapeutic biomarkers.

scholarly journals Toxicity of Povidone-Iodine to the Ocular Surface of Rabbits

2019 ◽  
Author(s):  
Sun Young Kim ◽  
Yong Sun Ahn ◽  
Yeo Jin Lee ◽  
Hyun Seung Kim
Keyword(s):  
Ocular Surface ◽  
Povidone Iodine ◽  
Study Groups ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Dependent Manner ◽  
Impression Cytology ◽  
Schirmer Test ◽  
Phosphate Buffered Saline ◽  
Goblet Cell Density

Abstract Background We evaluated the toxicity of 5% (w/v) povidone-iodine (PI) applied to the ocular surface of rabbits. Methods Twenty three white rabbits were divided into four groups; these were a control group and three study groups in which the ocular surface was exposed to PI for different times. In control group, phosphate-buffered saline (PBS) was applied once for 10 minutes. In study groups, 5% (w/v) PI was topically applied once for 1 minute, 3 minutes, and 10 minutes, and then the animals were observed for 7 days. The Schirmer test, Rose Bengal staining, corneal fluorescein staining and conjunctival impression cytology were performed on day 0, 3, and 7. After 7 days, the rabbits were sacrificed and conjunctiva and cornea were collected and evaluated by light and electron microscope. Immunofluorescence staining was also performed to detect mucin 5 subtype AC (MUC5AC). Results The decrease in goblet cell density, reductions in MUC5AC level and histopathological and ultrastructural changes of conjunctiva and cornea were more prominent in the 5% (w/v) PI groups than the control group (p < 0.05). Moreover, these changes were more prominent when PI was applied for 3 and 10 minutes rather than 1 minute (both p values < 0.05). Conclusions 5% (w/v) povidone-iodine caused damages to the ocular surface in a time-dependent manner. Therefore, we should be aware of that excessive PI exposure during ophthalmic procedures could be a pathogenic factor of dry eye syndrome after surgery.

scholarly journals The Use of Impression Cytology in Ocular Surface Diseases

2019 ◽  
Vol 1 (35) ◽  
pp. 43-46 ◽  
Author(s):  
Gulay Turan ◽  
Pembe Oltulu ◽  
Meydan Turan ◽  
Refik Oltulu
Keyword(s):  
Ocular Surface ◽  
Impression Cytology ◽  
Ocular Surface Diseases
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