scholarly journals Expression of a mitochondrial progesterone receptor in human spermatozoa correlates with a progestin-dependent increase in mitochondrial membrane potential

Andrology ◽  
2014 ◽  
Vol 2 (6) ◽  
pp. 875-883 ◽  
Author(s):  
J. Tantibhedhyangkul ◽  
K. C. Hawkins ◽  
Q. Dai ◽  
K. Mu ◽  
C. N. Dunn ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Progesterone Receptor ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Human Spermatozoa

scholarly journals Testicular human spermatozoa cryopreservation correlation between sperm head vacuoles, DNA fragmentation and mitochondrial membrane potential

2018 ◽  
Vol 23 (4) ◽  
pp. 413-417
Author(s):  
Sahabeh Etebary ◽  
Nahid Yari ◽  
Mohammad Ali Khalili ◽  
Seyed Mehdi Kalantar ◽  
Morteza Anvari
Keyword(s):  
Membrane Potential ◽  
Dna Fragmentation ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Sperm Head ◽  
Human Spermatozoa

scholarly journals Effect of oxidation-reduction potential on mitochondrial membrane potential and vitality of physiologically normal human spermatozoa

2019 ◽  
Vol 112 (3) ◽  
pp. e375 ◽  
Author(s):  
Manesh Kumar Panner Selvam ◽  
Ashok Agarwal ◽  
Renata Finelli ◽  
Christopher M. Douglas ◽  
Ralf Henkel ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Reduction Potential ◽  
Human Spermatozoa ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Normal Human

Aquaporin3 expression and the potential role of aquaporins in motility and mitochondrial membrane potential in human spermatozoa

Andrologia ◽  
2020 ◽  
Vol 52 (6) ◽  
Author(s):  
Atieh Alyasin ◽  
Hamid Reza Momeni ◽  
Majid Mahdieh
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Potential Role ◽  
Human Spermatozoa

scholarly journals Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose

Reproduction ◽  
2008 ◽  
Vol 136 (2) ◽  
pp. 167-173 ◽  
Author(s):  
E Isachenko ◽  
V Isachenko ◽  
J M Weiss ◽  
R Kreienberg ◽  
I I Katkov ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Acrosome Reaction ◽  
Staining Technique ◽  
Significant Loss ◽  
Human Spermatozoa ◽  
Mitochondrial Activity ◽  
Hoechst 33258 ◽  
Gentle Agitation

This study investigates the ability of sucrose to protect spermatozoa against mitochondrial damage, artificial cryoinduction of capacitation, and acrosome reaction. Spermatozoa were isolated using the swim-up procedure performed using three different media: (a) human tubal fluid (HTF, control) medium; (b) HTF with 1% human serum albumin (HSA); and (c) HTF with 1% HSA and 0.25 M sucrose. From each group, 30 μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by quickly submerging the spheres in HTF with 1% HSA at 37 °C with gentle agitation. Sperm motility, viability, mitochondrial membrane potential integrity, spontaneous capacitation, and acrosome reaction were investigated. Sperm viability, acrosome reaction, and capacitation were detected using the double fluorescence chlortetracycline-Hoechst 33258 staining technique. Mitochondrial function was evaluated using a unique fluorescent cationic dye, 5,5′,6,6′-tetrachloro-1-1′,3,3′-tetraethyl-benzamidazolocarbocyanin iodide, commonly known as JC-1. The number of progressively motile spermatozoa was significantly higher in the sucrose-supplemented medium group (57.1±3.2%,P<0.05) when compared with controls (19.4±1.9%). The combination of HSA and sucrose (65.2±2.6%) has a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P<0.05) compared with HSA alone (32.6±4.7%). In conclusion, vitrification of human spermatozoa with non-permeable cryoprotectants such as HSA and sucrose can effectively cryopreserve the cells without significant loss of important physiological parameters.

scholarly journals Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

2014 ◽  
Vol 21 (3) ◽  
pp. 237-243 ◽  
Author(s):  
P. Uribe ◽  
R. Boguen ◽  
F. Treulen ◽  
R. Sánchez ◽  
J.V. Villegas
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Nitrosative Stress ◽  
Human Spermatozoa

scholarly journals Comparison of four fluorochromes for the detection of the inner mitochondrial membrane potential in human spermatozoa and their correlation with sperm motility

2004 ◽  
Vol 19 (10) ◽  
pp. 2267-2276 ◽  
Author(s):  
Carole Marchetti ◽  
Nathalie Jouy ◽  
Brigitte Leroy-Martin ◽  
André Defossez ◽  
Pierre Formstecher ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Sperm Motility ◽  
Mitochondrial Membrane ◽  
Human Spermatozoa ◽  
Inner Mitochondrial Membrane
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