scholarly journals Effect of oxidation-reduction potential on mitochondrial membrane potential and vitality of physiologically normal human spermatozoa

2019 ◽  
Vol 112 (3) ◽  
pp. e375 ◽  
Author(s):  
Manesh Kumar Panner Selvam ◽  
Ashok Agarwal ◽  
Renata Finelli ◽  
Christopher M. Douglas ◽  
Ralf Henkel ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Reduction Potential ◽  
Human Spermatozoa ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential ◽  
Normal Human

scholarly journals Acrosomal status and mitochondrial activity of human spermatozoa vitrified with sucrose

Reproduction ◽  
2008 ◽  
Vol 136 (2) ◽  
pp. 167-173 ◽  
Author(s):  
E Isachenko ◽  
V Isachenko ◽  
J M Weiss ◽  
R Kreienberg ◽  
I I Katkov ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Acrosome Reaction ◽  
Staining Technique ◽  
Significant Loss ◽  
Human Spermatozoa ◽  
Mitochondrial Activity ◽  
Hoechst 33258 ◽  
Gentle Agitation

This study investigates the ability of sucrose to protect spermatozoa against mitochondrial damage, artificial cryoinduction of capacitation, and acrosome reaction. Spermatozoa were isolated using the swim-up procedure performed using three different media: (a) human tubal fluid (HTF, control) medium; (b) HTF with 1% human serum albumin (HSA); and (c) HTF with 1% HSA and 0.25 M sucrose. From each group, 30 μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by quickly submerging the spheres in HTF with 1% HSA at 37 °C with gentle agitation. Sperm motility, viability, mitochondrial membrane potential integrity, spontaneous capacitation, and acrosome reaction were investigated. Sperm viability, acrosome reaction, and capacitation were detected using the double fluorescence chlortetracycline-Hoechst 33258 staining technique. Mitochondrial function was evaluated using a unique fluorescent cationic dye, 5,5′,6,6′-tetrachloro-1-1′,3,3′-tetraethyl-benzamidazolocarbocyanin iodide, commonly known as JC-1. The number of progressively motile spermatozoa was significantly higher in the sucrose-supplemented medium group (57.1±3.2%,P<0.05) when compared with controls (19.4±1.9%). The combination of HSA and sucrose (65.2±2.6%) has a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P<0.05) compared with HSA alone (32.6±4.7%). In conclusion, vitrification of human spermatozoa with non-permeable cryoprotectants such as HSA and sucrose can effectively cryopreserve the cells without significant loss of important physiological parameters.

scholarly journals Redox regulation of mitochondrial functional activity by quinones

2016 ◽  
Vol 103 (4) ◽  
pp. 439-458 ◽  
Author(s):  
NG Krylova ◽  
TA Kulahava ◽  
VT Cheschevik ◽  
IK Dremza ◽  
GN Semenkova ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Electron Transport ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Function ◽  
Mitochondrial Membrane ◽  
Reduction Potential ◽  
Electron Flow ◽  
Rat Liver Mitochondria ◽  
Ros Generation ◽  
Intact Cells

Quinones are among the rare compounds successfully used as therapeutic agents to correct mitochondrial diseases and as specific regulators of mitochondrial function within cells. The aim of the present study was to elucidate the redox-dependent effects of quinones on mitochondrial function. The functional parameters [respiratory activity, membrane potential, and reactive oxygen species (ROS) generation] of isolated rat liver mitochondria and mitochondria in intact cells were measured in the presence of eight exogenously applied quinones that differ in lipophilicity and one-electron reduction potential. The quinones affected the respiratory parameters of mitochondria, and dissipated the mitochondrial membrane potential as well as influenced (either decreased or enhanced) ROS generation, and restored the electron flow during electron transport chain inhibition. The stimulation of ROS production by juglone and 2,5-di-tert-butyl-1,4-benzoquinone was accompanied by a decrease in the acceptor control and respiration control ratios, dissipation of the mitochondrial membrane potential and induction of the reverse electron flow under succinate oxidation in isolated mitochondria. Menadione and 2,3,5-trimethyl-1,4-benzoquinone, which decreased the mitochondrial ROS generation, did not affect the mitochondrial potential and, vice versa, were capable of restoring electron transport during Complex I inhibition. In intact C6 cells, all the quinones, except for coenzyme Q10, decreased the mitochondrial membrane potential. Juglone, 1,4-benzoquinone, and menadione showed the most pronounced effects. These findings indicate that quinones with the reduction potential values E1/2 in the range from −99 to −260 mV were effective redox regulators of mitochondrial electron transport.

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