Heterologous production of Pseudomonas aeruginosa rhamnolipid under anaerobic conditions for microbial enhanced oil recovery

2014 ◽  
Vol 118 (2) ◽  
pp. 379-389 ◽  
Author(s):  
F. Zhao ◽  
R. Shi ◽  
J. Zhao ◽  
G. Li ◽  
X. Bai ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Heterologous Production ◽  
Microbial Enhanced Oil Recovery ◽  
Anaerobic Conditions

Production of biosurfactant by a Pseudomonas aeruginosa isolate and its applicability to in situ microbial enhanced oil recovery under anoxic conditions

RSC Advances ◽  
2015 ◽  
Vol 5 (45) ◽  
pp. 36044-36050 ◽  
Author(s):  
Feng Zhao ◽  
Jie Zhang ◽  
Rongjiu Shi ◽  
Siqin Han ◽  
Fang Ma ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Microbial Enhanced Oil Recovery ◽  
Anoxic Conditions

An isolated strain SG can produce biosurfactant under anoxic conditions and has great potential forin situmicrobial enhanced oil recovery.

scholarly journals VARIATION OF CARBON SOURCES IN PRODUCTING RHAMNOLIPID BY Pseudomonas aeruginosa FOR MICROBIAL ENHANCED OIL RECOVER�S APPLICATION (VARIASI SUMBER KARBON PADA PRODUKSI RHAMNOLIPID OLEH Pseudomonas aeruginosa DALAM APLIKASI MICROBIAL ENHANCED OIL RECOVERY (MEOR))

2018 ◽  
Vol 40 (1) ◽  
pp. 33-40
Author(s):  
Nafian Awaludin ◽  
Cut Nanda Sari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Carbon Sources ◽  
Oil Production ◽  
Waste Cooking Oil ◽  
Microbial Enhanced Oil Recovery ◽  
Interface Tension ◽  
Cooking Oil ◽  
Production Wells

The decrease in oil production is caused by the ageing of oil production wells. The enhanced oil recovery (EOR) technology is proven to increase oil reserves and production in mature oil fields. One EOR technology that has proven to be efficient in increasing oil production is microbial EOR by using biosurfactant. The most effective biosurfactant is rhamnolipid produced by Pseudomonas aeruginosa, the bacteria of which can lower the interfacial tension between the petroleum and water. In biosurfactants production thanks to these bacteria, the substrate as the source of carbon in the fermentation process is needed. The sources of carbon used in this study are glucose, glycerol, molasses, banana peels, and waste from Pseudomonas aeruginosa by using Busnell Hass medium as a liquid medium of bacterial growth. Biosurfactants production results are; 74mg/L from glucose; 63mg/L from banana peels; 66mg / L from glycerol; 85mg/L from waste cooking oil; and 64mg/L of molasses with the following decreasing surface tension: 33.55 mN/m from glucose; 32.51 mN/m from banana peels; 27.55 mN/m from glycerol; 22.46 mN/m from waste cooking oil; and 31.49 mN/m from molasses. In addition, the decrease of interface tension of glucose; banana peels; glycerol; waste cooking oil; and molasses are as follows : 15.2 mN/m; 13.78 mN/m; 8:15 mN/m; 0.14 mN/m; and 11.2 mN/m respectively.Menurunnya produksi minyak bumi disebabkan karena sumur produksi yang sudah tua. Teknologi enhanced oil recovery (EOR) terbukti mampu meningkatkan cadangan dan produksi lapangan minyak mature. Salah satu teknologi EOR yang dikenal efi sien dalam meningkatkan perolehan minyak adalah microbial enhanced oil recovery menggunakan biosurfaktan. Biosurfaktan yang paling efektif adalah rhamnolipid yang dihasilkan oleh bakteri Pseudomonas aeruginosa yang dapat menurunkan tegangan antarmuka antara minyak bumi dengan air. Dalam produksi biosurfaktan oleh bakteri ini, diperlukan substrat sebagai sumber karbon dalam proses fermentasi. Sumber karbon yang digunakan pada penelitian ini adalah glukosa, gliserol, molase, kulit pisang, dan minyak jelantah. Penelitian ini bertujuan untuk mengetahui sumber karbon yang paling optimum dalam menghasilkan biosurfaktan dari Pseudomonas aeruginosa dengan menggunakan busnell hass medium sebagai media cair pertumbuhan bakteri. Produksi biosurfaktan yang dihasilkan adalah 74mg/L dari glukosa; 63mg/L dari kulit pisang; 66mg/L dari gliserol; 85mg/L dari minyak jelantah; dan 64mg/L dari molase dengan penurunan tegangan permukaan berturutturut: 33,55 mN/m dari glukosa; 32,51 mN/m dari kulit pisang; 27,55 mN/m dari gliserol; 22,46 mN/m dari minyak jelantah; dan 31,49 mN/m serta memiliki penurunan tegangan antarmuka dari glukosa; kulit pisang; glisero; minyak jelantah; dan molase berturut-turut adalah 15,2 mN/m; 13,78 mN/m; 8,15 mN/m; 0,14 mN/m; dan 11,2 mN/m.

scholarly journals Microbial Lipopeptide-Producing Strains and Their Metabolic Roles under Anaerobic Conditions

2021 ◽  
Vol 9 (10) ◽  
pp. 2030
Author(s):  
Jia-Yi Li ◽  
Lu Wang ◽  
Yi-Fan Liu ◽  
Lei Zhou ◽  
Hong-Ze Gang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Oil Recovery ◽  
Oil Reservoirs ◽  
Microbial Enhanced Oil Recovery ◽  
Bacterial Strains ◽  
Anaerobic Conditions ◽  
Peptide Synthetases ◽  
Structural Analogues ◽  
Lipopeptide Biosurfactants

The lipopeptide produced by microorganisms is one of the representative biosurfactants and is characterized as a series of structural analogues of different families. Thirty-four families covering about 300 lipopeptide compounds have been reported in the last decades, and most of the reported lipopeptides produced by microorganisms were under aerobic conditions. The lipopeptide-producing strains under anaerobic conditions have attracted much attention from both the academic and industrial communities, due to the needs and the challenge of their applications in anaerobic environments, such as in oil reservoirs and in microbial enhanced oil recovery (MEOR). In this review, the fifty-eight reported bacterial strains, mostly isolated from oil reservoirs and dominated by the species Bacillus subtilis, producing lipopeptide biosurfactants, and the species Pseudomonas aeruginosa, producing glycolipid biosurfactants under anaerobic conditions were summarized. The metabolic pathway and the non-ribosomal peptide synthetases (NRPSs) of the strain Bacillus subtilis under anaerobic conditions were analyzed, which is expected to better understand the key mechanisms of the growth and production of lipopeptide biosurfactants of such kind of bacteria under anaerobic conditions, and to expand the industrial application of anaerobic biosurfactant-producing bacteria.

A MATHEMATICAL MODEL OF MICROBIAL ENHANCED OIL RECOVERY IN POROUS MEDIA

2017 ◽  
Vol 8 (4) ◽  
pp. 263-272
Author(s):  
Jianlong Xiu ◽  
Tianyuan Wang ◽  
Ying Guo ◽  
Qingfeng Cui ◽  
Lixin Huang ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Porous Media ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Microbial Enhanced Oil Recovery
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