scholarly journals VARIATION OF CARBON SOURCES IN PRODUCTING RHAMNOLIPID BY Pseudomonas aeruginosa FOR MICROBIAL ENHANCED OIL RECOVER�S APPLICATION (VARIASI SUMBER KARBON PADA PRODUKSI RHAMNOLIPID OLEH Pseudomonas aeruginosa DALAM APLIKASI MICROBIAL ENHANCED OIL RECOVERY (MEOR))

2018 ◽  
Vol 40 (1) ◽  
pp. 33-40
Author(s):  
Nafian Awaludin ◽  
Cut Nanda Sari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Carbon Sources ◽  
Oil Production ◽  
Waste Cooking Oil ◽  
Microbial Enhanced Oil Recovery ◽  
Interface Tension ◽  
Cooking Oil ◽  
Production Wells

The decrease in oil production is caused by the ageing of oil production wells. The enhanced oil recovery (EOR) technology is proven to increase oil reserves and production in mature oil fields. One EOR technology that has proven to be efficient in increasing oil production is microbial EOR by using biosurfactant. The most effective biosurfactant is rhamnolipid produced by Pseudomonas aeruginosa, the bacteria of which can lower the interfacial tension between the petroleum and water. In biosurfactants production thanks to these bacteria, the substrate as the source of carbon in the fermentation process is needed. The sources of carbon used in this study are glucose, glycerol, molasses, banana peels, and waste from Pseudomonas aeruginosa by using Busnell Hass medium as a liquid medium of bacterial growth. Biosurfactants production results are; 74mg/L from glucose; 63mg/L from banana peels; 66mg / L from glycerol; 85mg/L from waste cooking oil; and 64mg/L of molasses with the following decreasing surface tension: 33.55 mN/m from glucose; 32.51 mN/m from banana peels; 27.55 mN/m from glycerol; 22.46 mN/m from waste cooking oil; and 31.49 mN/m from molasses. In addition, the decrease of interface tension of glucose; banana peels; glycerol; waste cooking oil; and molasses are as follows : 15.2 mN/m; 13.78 mN/m; 8:15 mN/m; 0.14 mN/m; and 11.2 mN/m respectively.Menurunnya produksi minyak bumi disebabkan karena sumur produksi yang sudah tua. Teknologi enhanced oil recovery (EOR) terbukti mampu meningkatkan cadangan dan produksi lapangan minyak mature. Salah satu teknologi EOR yang dikenal efi sien dalam meningkatkan perolehan minyak adalah microbial enhanced oil recovery menggunakan biosurfaktan. Biosurfaktan yang paling efektif adalah rhamnolipid yang dihasilkan oleh bakteri Pseudomonas aeruginosa yang dapat menurunkan tegangan antarmuka antara minyak bumi dengan air. Dalam produksi biosurfaktan oleh bakteri ini, diperlukan substrat sebagai sumber karbon dalam proses fermentasi. Sumber karbon yang digunakan pada penelitian ini adalah glukosa, gliserol, molase, kulit pisang, dan minyak jelantah. Penelitian ini bertujuan untuk mengetahui sumber karbon yang paling optimum dalam menghasilkan biosurfaktan dari Pseudomonas aeruginosa dengan menggunakan busnell hass medium sebagai media cair pertumbuhan bakteri. Produksi biosurfaktan yang dihasilkan adalah 74mg/L dari glukosa; 63mg/L dari kulit pisang; 66mg/L dari gliserol; 85mg/L dari minyak jelantah; dan 64mg/L dari molase dengan penurunan tegangan permukaan berturutturut: 33,55 mN/m dari glukosa; 32,51 mN/m dari kulit pisang; 27,55 mN/m dari gliserol; 22,46 mN/m dari minyak jelantah; dan 31,49 mN/m serta memiliki penurunan tegangan antarmuka dari glukosa; kulit pisang; glisero; minyak jelantah; dan molase berturut-turut adalah 15,2 mN/m; 13,78 mN/m; 8,15 mN/m; 0,14 mN/m; dan 11,2 mN/m.

scholarly journals Effectiveness of In-Situ Microbial Enhanced Oil Recovery in a Post-Polymer Flooded Reservoir

2018 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Injection Pressure ◽  
Oil Production ◽  
Microbial Enhanced Oil Recovery ◽  
Observation Area ◽  
Production Wells ◽  
Light Components ◽  
Dominant Bacteria

This study investigated the effectiveness of In-situ microbial enhanced oil recovery (IMEOR) in a post-polymer flooded oil reservoir located in SaNan oilfield, Northeast China. Two rounds of injection of nutrient medium were intermittently injected into the producing block and then monitored. The main results showed that the dominant bacteria of 4 production wells, Thauera of Beta-proteobacteria, Pseudomonas and Acinetobacter of Gamma-proteobacteria were directional activation, which showed a consistent enhancement. The abundance of Methanosaeta and Methanolinea increased, and showed a regular alternation with an increase of oil production. It contributed to production of bio-gas, leading to increasing of the injection pressure from 11.3 MPa to 13.9 MPa before the experiment which increased by more than 2.0 MPa. The contents of CO2 and CH4 varied alternately, and the variation was consistent with the order of injection of each activator. H2 was detected in the reservoir associated with the gas in the observation area. A large amount of enriched bio-gas was dissolved into and mixed with crude oil, which brought increasing of the proportion of light components in the whole hydrocarbon of the recovered oil. The other effect was activated microbial metabolites productions formed bio-plugs, which benefits for improving of the absorption profile and the production profile. A total of 6,243 t of incremental oil production was achieved, and an oil recovery rate increased by 3.93% (OOIP) to the end of 2015. Our trial suggested that IMEOR can be implemented for effective enhancement of further oil recovery from polymer flooded oil reservoirs.

scholarly journals Synthesis of nano emulsion from waste cooking oil for enhanced oil recovery applications

2019 ◽  
Vol 606 ◽  
pp. 012004 ◽  
Author(s):  
M I I M Noor ◽  
A Islam ◽  
S Saalah ◽  
C C Ken ◽  
S M Anisuzzaman ◽  
...  
Keyword(s):  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Waste Cooking Oil ◽  
Cooking Oil ◽  
Nano Emulsion

Production of biosurfactant by a Pseudomonas aeruginosa isolate and its applicability to in situ microbial enhanced oil recovery under anoxic conditions

RSC Advances ◽  
2015 ◽  
Vol 5 (45) ◽  
pp. 36044-36050 ◽  
Author(s):  
Feng Zhao ◽  
Jie Zhang ◽  
Rongjiu Shi ◽  
Siqin Han ◽  
Fang Ma ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Microbial Enhanced Oil Recovery ◽  
Anoxic Conditions

An isolated strain SG can produce biosurfactant under anoxic conditions and has great potential forin situmicrobial enhanced oil recovery.

scholarly journals Anaerobic biosynthesis of rhamnolipids by Pseudomonas aeruginosa: performance, mechanism and its application potential for enhanced oil recovery

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Feng Zhao ◽  
Qingzhi Wang ◽  
Ying Zhang ◽  
Liying Lei
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Biosynthetic Pathway ◽  
Carbon Sources ◽  
Oil Reservoirs ◽  
Core Flooding ◽  
Expression Of Genes ◽  
Key Genes ◽  
Anaerobic Production

Abstract Background Pseudomonas aeruginosa, the rhamnolipids-producer, is one of dominant bacteria in oil reservoirs. Although P. aeruginosa strains are facultative bacteria, the anaerobic biosynthesis mechanism of rhamnolipids is unclear. Considering the oxygen scarcity within oil reservoirs, revealing the anaerobic biosynthesis mechanism of rhamnolipids are significant for improving the in-situ production of rhamnolipids in oil reservoirs to enhance oil recovery. Results Pseudomonasaeruginosa SG anaerobically produced rhamnolipids using glycerol rather than glucose as carbon sources. Two possible hypotheses on anaerobic biosynthesis of rhamnolipids were proposed, the new anaerobic biosynthetic pathway (hypothesis 1) and the highly anaerobic expression of key genes (hypothesis 2). Knockout strain SGΔrmlB failed to anaerobically produce rhamnolipids using glycerol. Comparative transcriptomics analysis results revealed that glucose inhibited the anaerobic expression of genes rmlBDAC, fabABG, rhlABRI, rhlC and lasI. Using glycerol as carbon source, the anaerobic expression of key genes in P. aeruginosa SG was significantly up-regulated. The anaerobic biosynthetic pathway of rhamnolipids in P. aeruginosa SG were confirmed, involving the gluconeogenesis from glycerol, the biosynthesis of dTDP-l-rhamnose and β-hydroxy fatty acids, and the rhamnosyl transfer process. The engineered strain P. aeruginosa PrhlAB constructed in previous work enhanced 9.67% of oil recovery higher than the wild-type strain P. aeruginosa SG enhancing 8.33% of oil recovery. Conclusion The highly anaerobic expression of key genes enables P. aeruginosa SG to anaerobically biosynthesize rhamnolipids. The genes, rmlBDAC, fabABG, rhlABRI, rhlC and lasI, are key genes for anaerobic biosynthesis of rhamnolipid by P. aeruginosa. Improving the anaerobic production of rhamnolipids better enhanced oil recovery in core flooding test. This study fills the gaps in the anaerobic biosynthesis mechanism of rhamnolipids. Results are significant for the metabolic engineering of P. aeruginosa to enhance anaerobic production of rhamnolipids.

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