The effect of allogenic human Wharton's jelly stem cells seeded onto acellular dermal matrix in healing of rat burn wounds

2019 ◽  
Vol 19 (4) ◽  
pp. 995-1001 ◽  
Author(s):  
Mehra Nazempour ◽  
Davood Mehrabani ◽  
Rouhollah Mehdinavaz‐Aghdam ◽  
Seyedeh‐Sara Hashemi ◽  
Amin Derakhshanfar ◽  
...  
2009 ◽  
Vol 610-613 ◽  
pp. 1298-1301
Author(s):  
Xiang Rong Zhang ◽  
De Wu Liu ◽  
Guang Hua Guo ◽  
Yan Peng

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.


2018 ◽  
Vol 118 ◽  
pp. 330-335
Author(s):  
Erika Okuno ◽  
Isabele Carrilho Jarros ◽  
Patricia Souza Bonfim-Mendonça ◽  
Glória Vicente de Rezende ◽  
Melyssa Negri ◽  
...  

2009 ◽  
Vol 15 (7) ◽  
pp. 1833-1841 ◽  
Author(s):  
Björn Schönmeyr ◽  
Nicholas Clavin ◽  
Tomer Avraham ◽  
Valerie Longo ◽  
Babak J. Mehrara

2012 ◽  
Vol 69 (6) ◽  
pp. 656-662 ◽  
Author(s):  
Sheng-Ping Huang ◽  
Chia-Chun Hsu ◽  
Shun-Cheng Chang ◽  
Chih-Hsin Wang ◽  
Shou-Cheng Deng ◽  
...  

Burns ◽  
2019 ◽  
Vol 45 (7) ◽  
pp. 1685-1694 ◽  
Author(s):  
Yongjun Qi ◽  
Zhengxue Dong ◽  
Hongzhen Chu ◽  
Qi Zhao ◽  
Xiao Wang ◽  
...  

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