scholarly journals Skeletal muscle myosin and cardiac myosin attenuate heparin's antithrombin‐dependent anticoagulant activity

2020 ◽  
Author(s):  
Shravan Morla ◽  
Hiroshi Deguchi ◽  
John H. Griffin
Keyword(s):  
Skeletal Muscle ◽  
Anticoagulant Activity ◽  
Cardiac Myosin ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

scholarly journals Activated protein C anticoagulant activity is enhanced by skeletal muscle myosin

Haematologica ◽  
2019 ◽  
Vol 105 (8) ◽  
pp. e424-e427 ◽  
Author(s):  
Mary J. Heeb ◽  
José A. Fernández ◽  
Atsuki Yamashita ◽  
Olivia R. McDowell ◽  
Zihan Guo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein C ◽  
Anticoagulant Activity ◽  
Activated Protein C ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

scholarly journals Novel blood coagulation molecules: Skeletal muscle myosin and cardiac myosin

2020 ◽  
Vol 19 (1) ◽  
pp. 7-19 ◽  
Author(s):  
Hiroshi Deguchi ◽  
Shravan Morla ◽  
John H. Griffin
Keyword(s):  
Skeletal Muscle ◽  
Blood Coagulation ◽  
Cardiac Myosin ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

scholarly journals Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene.

1985 ◽  
Vol 82 (21) ◽  
pp. 7183-7187 ◽  
Author(s):  
A. Weydert ◽  
P. Daubas ◽  
I. Lazaridis ◽  
P. Barton ◽  
I. Garner ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Heavy Chain ◽  
Chain Gene ◽  
Myosin Heavy Chains ◽  
Cardiac Myosin ◽  
Heavy Chain Gene ◽  
Myosin Heavy Chain Gene ◽  
Skeletal Muscle Myosin ◽  
Heavy Chains ◽  
Muscle Myosin

scholarly journals Cardiac Myosin Promotes Thrombin Generation and Coagulation In Vitro and In Vivo

2020 ◽  
Vol 40 (4) ◽  
pp. 901-913 ◽  
Author(s):  
Jevgenia Zilberman-Rudenko ◽  
Hiroshi Deguchi ◽  
Meenal Shukla ◽  
Yoshimasa Oyama ◽  
Jennifer N. Orje ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Thrombin Generation ◽  
Factor Xa ◽  
Tissue Type ◽  
Cardiac Myosin ◽  
Skeletal Muscle Myosin ◽  
Coated Surfaces ◽  
Muscle Myosin

Objective: Cardiac myosin (CM) is structurally similar to skeletal muscle myosin, which has procoagulant activity. Here, we evaluated CM’s ex vivo, in vivo, and in vitro activities related to hemostasis and thrombosis. Approach and Results: Perfusion of fresh human blood over CM-coated surfaces caused thrombus formation and fibrin deposition. Addition of CM to blood passing over collagen-coated surfaces enhanced fibrin formation. In a murine ischemia/reperfusion injury model, exogenous CM, when administered intravenously, augmented myocardial infarction and troponin I release. In hemophilia A mice, intravenously administered CM reduced tail-cut-initiated bleeding. These data provide proof of concept for CM’s in vivo procoagulant properties. In vitro studies clarified some mechanisms for CM’s procoagulant properties. Thrombin generation assays showed that CM, like skeletal muscle myosin, enhanced thrombin generation in human platelet-rich and platelet-poor plasmas and also in mixtures of purified factors Xa, Va, and prothrombin. Binding studies showed that CM, like skeletal muscle myosin, directly binds factor Xa, supporting the concept that the CM surface is a site for prothrombinase assembly. In tPA (tissue-type plasminogen activator)-induced plasma clot lysis assays, CM was antifibrinolytic due to robust CM-dependent thrombin generation that enhanced activation of TAFI (thrombin activatable fibrinolysis inhibitor). Conclusions: CM in vitro is procoagulant and prothrombotic. CM in vivo can augment myocardial damage and can be prohemostatic in the presence of bleeding. CM’s procoagulant and antifibrinolytic activities likely involve, at least in part, its ability to bind factor Xa and enhance thrombin generation. Future work is needed to clarify CM’s pathophysiology and its mechanistic influences on hemostasis or thrombosis.

scholarly journals Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.

1985 ◽  
Vol 260 (20) ◽  
pp. 11275-11285 ◽  
Author(s):  
A M Edelman ◽  
K Takio ◽  
D K Blumenthal ◽  
R S Hansen ◽  
K A Walsh ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Skeletal Muscle Myosin ◽  
Calmodulin Binding ◽  
Catalytic Domains ◽  
Muscle Myosin

scholarly journals Function of skeletal muscle myosin heavy and light chain isoforms by an in vitro motility assay.

1993 ◽  
Vol 268 (27) ◽  
pp. 20414-20418
Author(s):  
S Lowey ◽  
G.S. Waller ◽  
K.M. Trybus
Keyword(s):  
Skeletal Muscle ◽  
Light Chain ◽  
Motility Assay ◽  
In Vitro Motility Assay ◽  
Skeletal Muscle Myosin ◽  
In Vitro Motility ◽  
Muscle Myosin

Cloning and in Situ Hybridization of Type 2A and 2B Rat Skeletal Muscle Myosin Tail Region: Implications for Filament Assembly

1993 ◽  
Vol 197 (3) ◽  
pp. 1312-1318 ◽  
Author(s):  
R.L. Lieber ◽  
S.C. Bodine ◽  
T.J. Burkholder ◽  
D.J. Pierotti ◽  
A.F. Ryan
Keyword(s):  
Skeletal Muscle ◽  
In Situ Hybridization ◽  
Rat Skeletal Muscle ◽  
Tail Region ◽  
Skeletal Muscle Myosin ◽  
Filament Assembly ◽  
Muscle Myosin
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