Enantioconvergent hydrolysis of m ‐nitrostyrene oxide at an elevated concentration by Phaseolus vulgaris epoxide hydrolase in the organic/aqueous two‐phase system

2020 ◽  
Vol 70 (3) ◽  
pp. 181-188
Author(s):  
Z. Wen ◽  
J. Zhao ◽  
Y.‐Y. Liu ◽  
J.‐J. Zhou ◽  
C. Liu ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Epoxide Hydrolase ◽  
Phase System ◽  
Elevated Concentration ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Enantioconvergent Hydrolysis ◽  
Hydrolysis Of

Enzymic Hydrolysis of Starch in Agitated PEG-Dextran Aqueous Two-Phase System

1996 ◽  
Vol 48 (7-8) ◽  
pp. 291-294 ◽  
Author(s):  
M. Liakopoulou-Kyriakides ◽  
A. Karakatsanis ◽  
M. Stamatoudis
Keyword(s):  
Phase System ◽  
Enzymic Hydrolysis ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Hydrolysis Of

Enzymatic hydrolysis of soluble starch in a polyethylene glycol-dextran aqueous two-phase system

1990 ◽  
Vol 69 (4) ◽  
pp. 240-243 ◽  
Author(s):  
Kazuhiko Hayashida ◽  
Koji Kunimoto ◽  
Fumihide Shiraishi ◽  
Koei Kawakami ◽  
Yasuhiko Arai
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Soluble Starch ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Hydrolysis Of

scholarly journals Substantially improving the enantioconvergence of PvEH1, a Phaseolus vulgaris epoxide hydrolase, towards m-chlorostyrene oxide by laboratory evolution

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Xun-Cheng Zong ◽  
Chuang Li ◽  
Yao-Hui Xu ◽  
Die Hu ◽  
Bo-Chun Hu ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Epoxide Hydrolase ◽  
Substrate Binding ◽  
Binding Pocket ◽  
Saturation Mutagenesis ◽  
Substrate Binding Pocket ◽  
Laboratory Evolution ◽  
E Coli ◽  
Enantioconvergent Hydrolysis ◽  
Hydrolysis Of

Abstract Background Epoxide hydrolase can regioselectively catalyze the oxirane ring-opening hydrolysis of rac-epoxides producing the corresponding chiral diols. In our laboratory, a gene named pveh1 encoding an EH from Phaseolus vulgaris was cloned. Although the directed modification of PvEH1 was carried out, the mutant PvEH1Y3 showed a limited degree of enantioconvergence towards racemic (rac-) m-chlorostyrene oxide (mCSO). Results PvEH1 and PvEH1Y3 were combinatively subjected to laboratory evolution to further enhance the enantioconvergence of PvEH1Y3 towards rac-mCSO. Firstly, the substrate-binding pocket of PvEH1 was identified using a CAVER 3.0 software, and divided into three zones. After all residues in zones 1 and 3 were subjected to leucine scanning, two E. coli transformants, E. coli/pveh1Y149L and /pveh1P184L, were selected, by which rac-mCSO was transformed into (R)-m-chlorophenyl-1,2-ethanediol (mCPED) having 55.1% and 27.2% eep. Secondly, two saturation mutagenesis libraries, E. coli/pveh1Y149X and /pveh1P184X (X: any one of 20 residues) were created at sites Y149 and P184 of PvEH1. Among all transformants, both E. coli/pveh1Y149L (65.8% αS and 55.1% eep) and /pveh1P184W (66.6% αS and 59.8% eep) possessed the highest enantioconvergences. Finally, the combinatorial mutagenesis was conducted by replacements of both Y149L and P184W in PvEH1Y3, constructing E. coli/pveh1Y3Z2, whose αS reached 97.5%, higher than that (75.3%) of E. coli/pveh1Y3. In addition, the enantioconvergent hydrolysis of 20 mM rac-mCSO was performed by E. coli/pveh1Y3Z2, giving (R)-mCPED with 95.2% eep and 97.2% yield. Conclusions In summary, the enantioconvergence of PvEH1Y3Z2 was successfully improved by laboratory evolution, which was based on the study of substrate-binding pocket by leucine scanning. Our present work introduced an effective strategy for the directed modification of enantioconvergence of PvEH1.

Enzymatic hydrolysis of cellulose recovered from ionic liquid-salt aqueous two-phase system

2020 ◽  
Vol 129 (5) ◽  
pp. 624-631 ◽  
Author(s):  
Kazuhiko Tanimura ◽  
Keishi Suga ◽  
Yukihiro Okamoto ◽  
Hiroshi Umakoshi
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Hydrolysis ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Liquid Salt ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Distribution of pigments in the aqueous two-phase system formed with piperazinium-based ionic liquid and anionic surfactant

2021 ◽  
Vol 330 ◽  
pp. 115677
Author(s):  
Xiaoxing Lu ◽  
Zhizhuo Lu ◽  
Rong Zhang ◽  
Lijiang Zhao ◽  
Hujun Xie
Keyword(s):  
Ionic Liquid ◽  
Anionic Surfactant ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System
Sign in / Sign up

Export Citation Format

Share Document