scholarly journals T rypanosoma brucei ( UMP synthase null mutants) are avirulent in mice, but recover virulence upon prolonged culture in vitro while retaining pyrimidine auxotrophy

2013 ◽  
Vol 90 (2) ◽  
pp. 443-455 ◽  
Author(s):  
Han B. Ong ◽  
Natasha Sienkiewicz ◽  
Susan Wyllie ◽  
Stephen Patterson ◽  
Alan H. Fairlamb
Keyword(s):  
Culture In Vitro ◽  
Prolonged Culture ◽  
Ump Synthase ◽  
Null Mutants

scholarly journals HIV-1 Evolves into a Nonsyncytium-Inducing Virus upon Prolonged Culture in Vitro

Virology ◽  
1999 ◽  
Vol 263 (1) ◽  
pp. 55-69 ◽  
Author(s):  
Atze T. Das ◽  
Aafke Land ◽  
Ineke Braakman ◽  
Bep Klaver ◽  
Ben Berkhout
Keyword(s):  
Culture In Vitro ◽  
Prolonged Culture ◽  
Hiv 1

Dynamics of morphofunctional changes in aging bovine ova during prolonged culture in vitro

2014 ◽  
Vol 8 (3) ◽  
pp. 258-266 ◽  
Author(s):  
I. Yu. Lebedeva ◽  
G. N. Singina ◽  
A. V. Lopukhov ◽  
N. A. Zinovieva
Keyword(s):  
Culture In Vitro ◽  
Prolonged Culture

scholarly journals Cleavage anomalies in early human embryos and survival after prolonged culture in-vitro

2000 ◽  
Vol 15 (12) ◽  
pp. 2634-2643 ◽  
Author(s):  
Mina Alikani ◽  
Gloria Calderon ◽  
Giles Tomkin ◽  
John Garrisi ◽  
Magdalena Kokot ◽  
...  
Keyword(s):  
Human Embryos ◽  
Culture In Vitro ◽  
Prolonged Culture ◽  
Early Human

The rod-to-coccoid body transformation in cultures of Helicobacter pylori

1990 ◽  
Vol 48 (3) ◽  
pp. 626-627
Author(s):  
A. R. Crooker ◽  
W. G. Kraft ◽  
T. L. Beard ◽  
M. C. Myers
Keyword(s):  
Helicobacter Pylori ◽  
Growth Cycle ◽  
Negative Bacterium ◽  
Body Formation ◽  
Coccoid Form ◽  
Spiral Form ◽  
Prolonged Culture ◽  
H Pylori ◽  
Upper Gastrointestinal

Helicobacter pylori is a microaerophilic, gram-negative bacterium found in the upper gastrointestinal tract of humans. There is strong evidence that H. pylori is important in the etiology of gastritis; the bacterium may also be a major predisposing cause of peptic ulceration. On the gastric mucosa, the organism exists as a spiral form with one to seven sheathed flagella at one (usually) or both poles. Short spirals were seen in the first successful culture of the organism in 1983. In 1984, Marshall and Warren reported a coccoid form in older cultures. Since that time, other workers have observed rod and coccal forms in vitro; coccoid forms predominate in cultures 3-7 days old. We sought to examine the growth cycle of H. pylori in prolonged culture and the mode of coccoid body formation.

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