scholarly journals Volatiles from Merino fleece evoke antennal and behavioural responses in the Australian sheep blow fly Lucilia cuprina

2019 ◽  
Vol 33 (4) ◽  
pp. 491-497
Author(s):  
G. Yan ◽  
S. Liu ◽  
A. C. Schlink ◽  
G. R. Flematti ◽  
B. S. Brodie ◽  
...  
2019 ◽  
Vol 295 (2) ◽  
pp. 287-298 ◽  
Author(s):  
Ying Yan ◽  
Megan E. Williamson ◽  
Rebecca J. Davis ◽  
Anne A. Andere ◽  
Christine J. Picard ◽  
...  

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ying Yan ◽  
Maxwell J. Scott

An amendment to this paper has been published and can be accessed via a link at the top of the paper.


2014 ◽  
Vol 51 ◽  
pp. 80-88 ◽  
Author(s):  
Fang Li ◽  
Holly A. Wantuch ◽  
Rebecca J. Linger ◽  
Esther J. Belikoff ◽  
Maxwell J. Scott

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 797
Author(s):  
Ying Yan ◽  
Megan E. Williamson ◽  
Maxwell J. Scott

The sterile insect technique (SIT) is a promising strategy to control the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. We have previously developed a transgenic embryonic sexing system (TESS) for this pest to facilitate the potential SIT application. TESS carry two transgenes, a tetracycline transactivator (tTA) driver and a tTA-activated pro-apoptotic effector. TESS females die at the embryonic stage unless tetracycline is supplied in the diet. However, undesired female sterility was observed in some TESS strains without tetracycline due to expression of tTA in ovaries. Here we investigate if TESS that combine transgenes with relatively low/moderate expression/activity improves the fertility of TESS females. tTA driver lines were evaluated for tTA expression by quantitative real time PCR and/or by crossing with a tTA-activated RFPex effector line. Fertility and lethality tests showed that a TESS strain containing a driver line with moderate tTA expression and an effector line showing moderate pro-apoptotic activity could recover the fertility of parental females and eliminated all female offspring at the embryonic stage. Consequently, such a strain could be further evaluated for an SIT program for L. cuprina, and such a “moderate strategy” could be considered for the TESS development in other pest species.


2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ying Yan ◽  
Maxwell J. Scott

Abstract Genetic approaches, including the sterile insect technique (SIT), have previously been considered for control of the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. In an SIT program, females consume 50% of the diet but are ineffective as control agents and compete with females in the field for mating with sterile males, thereby decreasing the efficiency of the program. Consequently, transgenic sexing strains of L. cuprina were developed that produce 100% males when raised on diet that lacks tetracycline. However, as females die mostly at the pupal stage, rearing costs would not be significantly reduced. Here we report the development of transgenic embryonic sexing strains of L. cuprina. In these strains, the Lsbnk cellularization gene promoter drives high levels of expression of the tetracycline transactivator (tTA) in the early embryo. In the absence of tetracycline, tTA activates expression of the Lshid proapoptotic gene, leading to death of the embryo. Sex-specific RNA splicing of Lshid transcripts ensures that only female embryos die. Embryonic sexing strains were also made by combining the Lsbnk-tTA and tetO-Lshid components into a single gene construct, which will facilitate transfer of the technology to other major calliphorid livestock pests.


2000 ◽  
Vol 75 (3) ◽  
pp. 257-267 ◽  
Author(s):  
G. R. WARMAN ◽  
R. D. NEWCOMB ◽  
R. D. LEWIS ◽  
C. W. EVANS

We have isolated a homologue of the period (per) gene from the Australian sheep blow fly, Lucilia cuprina, as part of a comparative approach to the analysis of dipteran circadian systems. Sequence analysis of the 4 kb per cDNA revealed the conservation of three functional domains, namely the PAS dimerization motif, and the nuclear and cytoplasmic localization domains. A fourth domain, the threonine–glycine (TG) repeat region, is also conserved in L. cuprina per but has been severely truncated. No length variation was found in the TG repeat of L. cuprina or L. sericata collected from several different latitudinal zones. Expression analysis indicated a diel oscillation in per mRNA in LD 12[ratio ]12 with a period of 24 h and a peak at Zt 12. PER-immunoreactive protein oscillations were also demonstrated, with peak immunoreactivity lagging approximately 3 h behind peak mRNA levels. These results show the existence of a Drosophila-like circadian system in a calliphorid fly. They also provide evidence for the conservation of per function across the Diptera, and confirm the relevance of the Drosophila system as a model for fly circadian rhythms.


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