scholarly journals Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato

2021 ◽  
Author(s):  
M. Victoria Barja ◽  
Miguel Ezquerro ◽  
Stefano Beretta ◽  
Gianfranco Diretto ◽  
Igor Florez‐Sarasa ◽  
...  
Keyword(s):  
Carotenoid Biosynthesis ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
Metabolic Substrates

scholarly journals Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato

2020 ◽  
Author(s):  
M. Victoria Barja ◽  
Miguel Ezquerro ◽  
Gianfranco Diretto ◽  
Igor Florez-Sarasa ◽  
Elisenda Feixes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carotenoid Biosynthesis ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
Network Analyses ◽  
Metabolic Substrates ◽  
Pathway Gene ◽  
Similar Kinetic ◽  
Carotenoid Pathway ◽  
Ggpp Synthase

ABSTRACTGeranylgeranyl diphosphate (GGPP) produced by GGPP synthase (GGPPS) serves as a precursor for many plastidial isoprenoids, including carotenoids. Here we show that five different GGPPS isoforms exist in tomato (Solanum lycopersicum). From these, SlGGPPS1, 2 and 3 (or SlG1-3 in short) produce GGPP in plastids and exhibit similar kinetic parameters. Phytoene synthase (PSY) converts GGPP into phytoene, the first committed intermediate of the carotenoid pathway. Gene expression and co-expression network analyses showed a preferential association of individual GGPPS and PSY isoforms in processes linked to carotenoid biosynthesis such as root mycorrhization, seedling deetiolation and fruit ripening. Co-immunoprecipitation experiments showed that SlG2, but not SlG3, physically interacts with PSY proteins. By contrast, CRISPR-Cas9 mutants defective in SlG3 showed a stronger impact on carotenoid levels and derived metabolic, physiological and developmental phenotypes that those impaired in SlG2. Double mutants with a simultaneous knockout of both genes could not be found. Our work demonstrates that the bulk of GGPP production in tomato chloroplasts and chromoplasts relies on two cooperating GGPPS paralogs, unlike other plant species such as Arabidopsis thaliana, rice or pepper, which produce their essential plastidial isoprenoids using a single GGPPS isoform.

Heteromeric Geranylgeranyl Diphosphate Synthase Contributes to Carotenoid Biosynthesis in Ripening Fruits of Red Pepper (Capsicum annuum var. conoides)

2018 ◽  
Vol 66 (44) ◽  
pp. 11691-11700 ◽  
Author(s):  
Qiang Wang ◽  
Xing-Qi Huang ◽  
Tian-Jun Cao ◽  
Zhong Zhuang ◽  
Ran Wang ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Carotenoid Biosynthesis ◽  
Red Pepper ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate

Cloning and characterization of the geranylgeranyl diphosphate synthase (GGPS) responsible for carotenoid biosynthesis in Pyropia umbilicalis

2015 ◽  
Vol 28 (1) ◽  
pp. 671-678 ◽  
Author(s):  
Li-En Yang ◽  
Xing-Qi Huang ◽  
Qin-Qin Lu ◽  
Jian-Yi Zhu ◽  
Shan Lu
Keyword(s):  
Carotenoid Biosynthesis ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate

scholarly journals Distribution of prenyltransferases in rat tissues. Evidence for a cytosolic all-trans-geranylgeranyl diphosphate synthase.

1993 ◽  
Vol 268 (2) ◽  
pp. 832-838
Author(s):  
J. Ericsson ◽  
M. Runquist ◽  
A. Thelin ◽  
M. Andersson ◽  
T. Chojnacki ◽  
...  
Keyword(s):  
Rat Tissues ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate

scholarly journals Lipophilic Bisphosphonates as Dual Farnesyl/Geranylgeranyl Diphosphate Synthase Inhibitors: An X-ray and NMR Investigation

2009 ◽  
Vol 131 (14) ◽  
pp. 5153-5162 ◽  
Author(s):  
Yonghui Zhang ◽  
Rong Cao ◽  
Fenglin Yin ◽  
Michael P. Hudock ◽  
Rey-Ting Guo ◽  
...  
Keyword(s):  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
X Ray

scholarly journals Maize cDNAs Expressed in Endosperm Encode Functional Farnesyl Diphosphate Synthase with Geranylgeranyl Diphosphate Synthase Activity

2006 ◽  
Vol 141 (1) ◽  
pp. 220-231 ◽  
Author(s):  
Miguel Cervantes-Cervantes ◽  
Cynthia E. Gallagher ◽  
Changfu Zhu ◽  
Eleanore T. Wurtzel
Keyword(s):  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate

Studies on geranylgeranyl diphosphate synthase from rat liver: Specific inhibition by 3-azageranylgeranyl diphosphate

1992 ◽  
Vol 297 (2) ◽  
pp. 314-320 ◽  
Author(s):  
Hiroshi Sagami ◽  
Tatsumi Korenaga ◽  
Kyozo Ogura ◽  
Arthur Steiger ◽  
Hyung-Jung Pyun ◽  
...  
Keyword(s):  
Rat Liver ◽  
Specific Inhibition ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate
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