scholarly journals Development and regeneration of wheat–rice hybrid zygotes produced by in vitro fertilization system

2021 ◽  
Author(s):  
Tety Maryenti ◽  
Takayoshi Ishii ◽  
Takashi Okamoto
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Rice Hybrid ◽  
Fertilization System

Male-dependent variability of fertilization and embryo development in two bovine in vitro fertilization systems and the effects of casein phosphopeptides (CPPs)

1997 ◽  
Vol 9 (4) ◽  
pp. 465 ◽  
Author(s):  
U. Kreysing ◽  
T. Nagai ◽  
H. Niemann
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Individual Variability ◽  
Bovine Embryo ◽  
Blastocyst Formation ◽  
Embryo Production ◽  
Vitro Fertilization ◽  
Casein Phosphopeptides ◽  
Fertilization System

This study investigated the effects of semen from five different bulls and two different ejaculates of the same bull on penetration, cleavage, blastocyst formation, and cell allocation in bovine blastocysts produced in vitro. Casein phosphopeptides (CPPs) were tested for their ability to enhance fertilization and minimize variability among bulls and ejaculates. In Experiment 1, the BO-fertilization system was employed. Penetration and polyspermy both displayed great variation among bulls and between ejaculates, whereas no significant differences were observed in cleavage and blastocyst-formation rates. Similar variability was observed in penetration, polyspermy, cleavage, blastocyst-formation rates and cell allocation and distribution when the two fertilization systems, TALP and BO, were compared in Experiment 2. The BO-system supported penetration and polyspermy better (P < 0·05) than the TALP-system, whereas the TALP-system was superior (P < 0·05) in supporting cleavage and blastocyst formation. Significant interactions existed between bulls and the fertilization system employed. It is concluded that the success of in vitrofertilization is markedly dependent on individual bulls as well as on ejaculates from the same bull. CPPs are able to enhance penetration and embryo development in certain bulls or ejaculates and thus contribute to reducing the degree of individual variability, but they do not generally improve the success of bovine embryo production in vitro.

Freeze-Fracture Observations of Hamster Sperm-Egg Fusion

1988 ◽  
Vol 46 ◽  
pp. 280-281
Author(s):  
Judy Clark ◽  
James K. Koehler
Keyword(s):  
In Vitro Fertilization ◽  
Freeze Fracture ◽  
Fusion Process ◽  
Thin Sections ◽  
Vitro Fertilization ◽  
Fertilization System ◽  
Sem Studies ◽  
Lipid Components

A number of TEM and SEM studies have made use of the hamster in vitro fertilization system to observe the process of sperm-egg fusion. We have extended these studies to include observations of freeze fracture replicas at the site of the sperm-egg interactions prior to, during and immediately following their fusion.Zona-free eggs were collected following superovulation of female hamsters according to the methods of Yanagimachi et al. Sperm were capacitated and prepared for incubation with eggs as in Clark et al. Eggs and sperm were co-incubated 5-15 minutes, washed and immediately fixed and prepared for freeze fracture replicas and thin sections as previously reported.In order to investigate the role of lipid components during the fusion process, some preparations were fixed in the presence of filipin, which binds to β-OH sterols in membranes and forms complexes that are visible in freeze fracture replicas. Thin sections were also prepared and viewed to help confirm and support observations of the replicas.

scholarly journals Species-Specific Sperm-Egg Interaction Affects the Utility of a Heterologous Bovine In Vitro Fertilization System for Evaluating Antelope Sperm

2001 ◽  
Vol 65 (4) ◽  
pp. 1246-1251 ◽  
Author(s):  
Andrew J. Kouba ◽  
Mark W. Atkinson ◽  
A. Rae Gandolf ◽  
Terri L. Roth
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Species Specific ◽  
Fertilization System

Sperm Phagocytosis by Corona Cells in a Human in Vitro Fertilization System

1985 ◽  
Vol 442 (1 In Vitro Fert) ◽  
pp. 310-317 ◽  
Author(s):  
R. PIJNENBORG ◽  
S. GORDTS ◽  
R. ONGKOWIDJOJO ◽  
I. BROSENS
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Human In Vitro Fertilization ◽  
Fertilization System

Establishment of an in vitro fertilization system in rice (Oryza sativa L.)

Planta ◽  
2007 ◽  
Vol 226 (3) ◽  
pp. 581-589 ◽  
Author(s):  
Takao Uchiumi ◽  
Isao Uemura ◽  
Takashi Okamoto
Keyword(s):  
Oryza Sativa ◽  
In Vitro Fertilization ◽  
Oryza Sativa L ◽  
Vitro Fertilization ◽  
Fertilization System

Fertilin β peptides inhibit sperm binding to zona-free eggs in a homologous baboon in vitro fertilization system

Contraception ◽  
1999 ◽  
Vol 59 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Peter Gichuhi Mwethera ◽  
Alloys Makokha ◽  
Daniel Chai
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Sperm Binding ◽  
Fertilization System

Establishment of an In Vitro Fertilization System in Wheat (Triticum aestivumL.)

2019 ◽  
Vol 60 (4) ◽  
pp. 835-843 ◽  
Author(s):  
Tety Maryenti ◽  
Norio Kato ◽  
Masako Ichikawa ◽  
Takashi Okamoto
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Fertilization System
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