Constitutive expression of a stabilized transcription factor group VII ethylene response factor enhances waterlogging tolerance in wheat without penalizing grain yield

2019 ◽  
Vol 42 (5) ◽  
pp. 1471-1485 ◽  
Author(s):  
Xuening Wei ◽  
Huijun Xu ◽  
Wei Rong ◽  
Xingguo Ye ◽  
Zengyan Zhang
Keyword(s):  
Transcription Factor ◽  
Grain Yield ◽  
Constitutive Expression ◽  
Factor Group ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Waterlogging Tolerance ◽  
Ethylene Response

scholarly journals Structural insights into DNA sequence recognition by Arabidopsis ETHYLENE RESPONSE FACTOR 96

2018 ◽  
Author(s):  
Chun-Yen Chen ◽  
Pei-Hsuan Lin ◽  
Kun-Hung Chen ◽  
Yi-Sheng Cheng
Keyword(s):  
Transcription Factor ◽  
Defense Responses ◽  
Structural Basis ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Recognition Sequence ◽  
Gcc Box ◽  
Sequence Recognition ◽  
Ethylene Response ◽  
Α Helix

ABSTRACTThe phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein (EREBP), a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, is critical for the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

scholarly journals A group VII ethylene response factor gene, ZmEREB180 , coordinates waterlogging tolerance in maize seedlings

2019 ◽  
Vol 17 (12) ◽  
pp. 2286-2298 ◽  
Author(s):  
Feng Yu ◽  
Kun Liang ◽  
Tian Fang ◽  
Hailiang Zhao ◽  
Xuesong Han ◽  
...  
Keyword(s):  
Ethylene Response Factor ◽  
Response Factor ◽  
Maize Seedlings ◽  
Waterlogging Tolerance ◽  
Factor Gene ◽  
Ethylene Response ◽  
Ethylene Response Factor Gene

scholarly journals Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

2020 ◽  
Vol 104 (4-5) ◽  
pp. 483-498
Author(s):  
Chun-Yen Chen ◽  
Pei-Hsuan Lin ◽  
Kun-Hung Chen ◽  
Yi-Sheng Cheng
Keyword(s):  
Transcription Factor ◽  
Protein A ◽  
Defense Responses ◽  
Structural Basis ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Recognition Sequence ◽  
Gcc Box ◽  
Ethylene Response ◽  
Α Helix

Abstract The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

scholarly journals Ethylene Response Factor 1 Mediates Arabidopsis Resistance to the Soilborne Fungus Fusarium oxysporum

2004 ◽  
Vol 17 (7) ◽  
pp. 763-770 ◽  
Author(s):  
Marta Berrocal-Lobo ◽  
Antonio Molina
Keyword(s):  
Fusarium Oxysporum ◽  
Constitutive Expression ◽  
Defense Responses ◽  
Transcriptional Factor ◽  
Signal Pathways ◽  
Ethylene Response Factor ◽  
Response Factor ◽  
Enhanced Resistance ◽  
Ethylene Response ◽  
Necrotrophic Fungi

Ethylene response factor 1 (ERF1) is a transcriptional factor from Arabidopsis thaliana that regulates plant resistance to the necrotrophic fungi Botrytis cinerea and Plectosphaerella cucumerina and whose overexpression enhances resistance to these fungi. Here, we show that ERF1 also mediates Arabidopsis resistance to the soilborne fungi Fusarium oxysporum sp. conglutinans and F. oxysporum f. sp. lycopersici, because its constitutive expression in Arabidopsis confers enhanced resistance to these pathogens. Expression of ERF1 was upregulated after inoculation with F. oxysporum f. sp. conglutinans, and this response was blocked in ein2-5 and coi1-1 mutants, impaired in the ethylene (ET) and jasmonic acid (JA) signal pathways, respectively, which further indicates that ERF1 is a downstream component of ET and JA defense responses. The signal transduction network controlling resistance to F. oxysporum fungi was explored using signaling-defective mutants in ET (ein2-5), JA (jar1-1), and salicylic acid (SA) (NahG, sid2-1, eds5-1, npr1-1, pad4-1, eds1-1, and pad2-1) transduction pathways. This analysis revealed that Arabidopsis resistance to F. oxysporum requires the ET, JA, and SA signaling pathways and the NPR1 gene, although it is independent of the PAD4 and EDS1 functions.

Overexpression of a pepper CaERF5 gene in tobacco plants enhances resistance to Ralstonia solanacearum infection

2014 ◽  
Vol 41 (7) ◽  
pp. 758 ◽  
Author(s):  
Yan Lai ◽  
Fengfeng Dang ◽  
Jing Lin ◽  
Lu Yu ◽  
Jinhui Lin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Amino Acid ◽  
Stress Responses ◽  
Ralstonia Solanacearum ◽  
Phosphorylation Site ◽  
Constitutive Expression ◽  
Ethylene Response Factor ◽  
Crop Species ◽  
Tobacco Plants ◽  
Ethylene Response

ETHYLENE RESPONSE FACTORs (ERF) transcription factors (TFs) constitute a large transcriptional regulator family belonging to the AP2/ERF superfamily and are implicated in a range of biological processes. However, the specific roles of individual ERF family members in biotic or abiotic stress responses and the underlying molecular mechanism still need to be elucidated. In the present study, a cDNA encoding a member of ethylene response factor (ERF) transcription factor, CaERF5, was isolated from pepper. Sequence analysis showed that CaERF5 contains a typical 59 amino acid AP2/ERF DNA-binding domain, two highly conserved amino acid residues (14th alanine (A) and 19th aspartic acid (D)), a putative nuclear localisation signal (NLS), a CMIX-2 motif in the N-terminal region and two putative MAP kinase phosphorylation site CMIX-5 and CMIX-6 motifs. It belongs to group IXb of the ERF subfamily. A CaERF5-green fluorescence protein (GFP) fusion transiently expressed in onion epidermal cells localised to the nucleus. CaERF5 transcripts were induced by Ralstonia solanacearum infection, salicylic acid (SA), methyl jasmonate (MeJA) and ethephon (ETH) treatments. Constitutive expression of the CaERF5 gene in tobacco plants upregulated transcript levels of a set of defence- related genes and enhanced resistance to R. solanacearum infection. Our results suggest that CaERF5 acts as a positive regulator in plant resistance to R. solanacearum infection and show that overexpression of this transcription factor can be used as a tool to enhance disease resistance in crop species.

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