The ethylene response factor Va ERF 092 from Amur grape regulates the transcription factor Va WRKY 33, improving cold tolerance

ABSTRACTThe phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein (EREBP), a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, is critical for the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

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BcERF070, a novel ERF (ethylene-response factor) transcription factor from non-heading Chinese cabbage, affects the accumulation of ascorbic acid by regulating ascorbic acid-related genes

Molecular Breeding ◽

10.1007/s11032-019-1065-5 ◽

2019 ◽

Vol 40 (1) ◽

Cited By ~ 1

Author(s):

Jingping Yuan ◽

Zhanghong Yu ◽

Tingting Lin ◽

Li Wang ◽

Xuan Chen ◽

...

Keyword(s):

Ascorbic Acid ◽

Transcription Factor ◽

Chinese Cabbage ◽

Ethylene Response Factor ◽

Response Factor ◽

Ethylene Response ◽

Heading Chinese Cabbage

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Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

Plant Molecular Biology ◽

10.1007/s11103-020-01052-5 ◽

2020 ◽

Vol 104 (4-5) ◽

pp. 483-498

Author(s):

Chun-Yen Chen ◽

Pei-Hsuan Lin ◽

Kun-Hung Chen ◽

Yi-Sheng Cheng

Keyword(s):

Transcription Factor ◽

Protein A ◽

Defense Responses ◽

Structural Basis ◽

Ethylene Response Factor ◽

Response Factor ◽

Recognition Sequence ◽

Gcc Box ◽

Ethylene Response ◽

Α Helix

Abstract The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

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