Skin Responses to Micro Scale Field Size of Solar-Simulated Radiation - Preliminary Evaluation by Reflectance Confocal Microscopyin vivo

Abstract Two-color laser induced fluorescence (LIF) technique has been developed and examined to use for full-field temperature mapping of a micro-scale field-of-view in water. The technique uses two fluorescence dyes with different emission characteristics — one is the temperature sensitive dye (Rhodamine-B) and the other is the temperature insensitive dye (Rhodamine-110). The ratio of the two fluorescence emission intensities, therefore, provides a formidable correlation with temperature that does not depend on the laser illumination intensity variation and is free from the possible bias occurring from background noise. In considering the technique for applications to micro-scale field-of-view, the first question that may be asked will be if the technique ensures acceptable accuracy in temperature readings with sufficiently small spatial resolution. In order to explore answers to this question, an extensive calibration for the intensity ratio versus temperature has been performed using a constant-temperature bath and the calibration results have been statistically analyzed to estimate measurement uncertainties. The developed technique measures thermally stratified fields with known temperature distributions that are established inside 10-mm and 1-mm path cuvettes to ensure measurement accuracy and spatial resolution for potential microscale applications.

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Preliminary Evaluation of a Weibull Function for Fitting Slow-Component Eye Velocity over the Time Course of Caloric-Induced Nystagmus

Journal of Speech Language and Hearing Research ◽

10.1044/jshr.3203.681 ◽

1989 ◽

Vol 32 (3) ◽

pp. 681-687 ◽

Cited By ~ 1

Author(s):

C. Formby ◽

B. Albritton ◽

I. M. Rivera

Keyword(s):

Time Course ◽

Slow Component ◽

Weibull Function ◽

Preliminary Evaluation ◽

Mathematical Function ◽

Before And After ◽

Best Fitting ◽

Eye Velocity ◽

Fitting Parameters ◽

Weibull Equation

We describe preliminary attempts to fit a mathematical function to the slow-component eye velocity (SCV) over the time course of caloric-induced nystagmus. Initially, we consider a Weibull equation with three parameters. These parameters are estimated by a least-squares procedure to fit digitized SCV data. We present examples of SCV data and fitted curves to show how adjustments in the parameters of the model affect the fitted curve. The best fitting parameters are presented for curves fit to 120 warm caloric responses. The fitting parameters and the efficacy of the fitted curves are compared before and after the SCV data were smoothed to reduce response variability. We also consider a more flexible four-parameter Weibull equation that, for 98% of the smoothed caloric responses, yields fits that describe the data more precisely than a line through the mean. Finally, we consider advantages and problems in fitting the Weibull function to caloric data.

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