Gating of the two-pore cation channel AtTPC1 in the plant vacuole is based on a single voltage-sensing domain

Plant Biology ◽  
2016 ◽  
Vol 18 (5) ◽  
pp. 750-760 ◽  
Author(s):  
D. Jaślan ◽  
T. D. Mueller ◽  
D. Becker ◽  
J. Schultz ◽  
T. A. Cuin ◽  
...  
Keyword(s):  
Cation Channel ◽  
Voltage Sensing ◽  
Plant Vacuole ◽  
Voltage Sensing Domain

scholarly journals The Isolated Voltage Sensing Domain of the Shaker Potassium Channel forms a Cation Channel

2017 ◽  
Vol 112 (3) ◽  
pp. 249a
Author(s):  
Juan Zhao ◽  
Rikard Blunck
Keyword(s):  
Potassium Channel ◽  
Cation Channel ◽  
Voltage Sensing ◽  
Shaker Potassium Channel ◽  
Voltage Sensing Domain

New Molecular Scaffolds for Fluorescent Voltage Indicators

2018 ◽  
Author(s):  
Steven Boggess ◽  
Shivaani Gandhi ◽  
Brian Siemons ◽  
Nathaniel Huebsch ◽  
Kevin Healy ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Action Potentials ◽  
Induced Pluripotent Stem Cell ◽  
Molecular Wire ◽  
Voltage Sensing ◽  
Design Synthesis ◽  
Cardiac Action ◽  
Action Potential Waveform ◽  
Induced Pluripotent ◽  
Voltage Sensing Domain

<div> <p>The ability to non-invasively monitor membrane potential dynamics in excitable cells like neurons and cardiomyocytes promises to revolutionize our understanding of the physiology and pathology of the brain and heart. Here, we report the design, synthesis, and application of a new class of fluorescent voltage indicator that makes use of a fluorene-based molecular wire as a voltage sensing domain to provide fast and sensitive measurements of membrane potential in both mammalian neurons and human-derived cardiomyocytes. We show that the best of the new probes, fluorene VoltageFluor 2 (fVF 2) readily reports on action potentials in mammalian neurons, detects perturbations to cardiac action potential waveform in human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes, shows a substantial decrease in phototoxicity compared to existing molecular wire-based indicators, and can monitor cardiac action potentials for extended periods of time. Together, our results demonstrate the generalizability of a molecular wire approach to voltage sensing and highlights the utility of fVF 2 for interrogating membrane potential dynamics.</p> </div>

Quantum Calculation of Proton and Other Charge Transfer Steps in Voltage Sensing in the Kv1.2 Channel

2019 ◽  
Author(s):  
Alisher M Kariev ◽  
Michael Green
Keyword(s):  
Charge Transfer ◽  
Energy Charge ◽  
Quantum Calculation ◽  
Quantum Calculations ◽  
Gating Current ◽  
Transmembrane Segment ◽  
Voltage Sensing ◽  
Standard Models ◽  
Charge Displacement ◽  
Voltage Sensing Domain

Quantum calculations on 976 atoms of the voltage sensing domain of the K<sub>v</sub>1.2 channel, with protons in several positions, give energy, charge transfer, and other properties. Motion of the S4 transmembrane segment that accounts for gating current in standard models is shown not to occur; there is H<sup>+ </sup>transfer instead. The potential at which two proton positions cross in energy approximately corresponds to the gating potential for the channel. The charge displacement seems approximately correct for the gating current. Two mutations are accounted for (Y266F, R300cit, cit =citrulline). The primary conclusion is that voltage sensing depends on H<sup>+</sup> transfer, not motion of arginine charges.

scholarly journals A Proton Pathway in the Voltage Sensing Domain of KV Channels, with a Possible Relation of Gating to the Pore Cavity and to the T1 Moiety

2011 ◽  
Vol 100 (3) ◽  
pp. 580a
Author(s):  
Alisher M. Kariev ◽  
Michael E. Green
Keyword(s):  
Voltage Sensing ◽  
Kv Channels ◽  
Voltage Sensing Domain

scholarly journals Light-induced Conformational Changes of LOV1 (Light Oxygen Voltage-sensing Domain 1) and LOV2 Relative to the Kinase Domain and Regulation of Kinase Activity inChlamydomonasPhototropin

2013 ◽  
Vol 289 (1) ◽  
pp. 413-422 ◽  
Author(s):  
Koji Okajima ◽  
Yusuke Aihara ◽  
Yuki Takayama ◽  
Mihoko Nakajima ◽  
Sachiko Kashojiya ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Kinase Activity ◽  
Kinase Domain ◽  
Voltage Sensing ◽  
Voltage Sensing Domain
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