Relationship between DNA fragmentation of equine granulosa cells and oocyte meiotic competence after in vitro maturation

2019 ◽  
Vol 54 ◽  
pp. 78-81
Author(s):  
Blasa Pereira ◽  
Jesús Dorado ◽  
Maria Diaz-Jimenez ◽  
Cesar Consuegra ◽  
Isabel Ortiz ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Meiotic Competence

scholarly journals MicroRNAs transfected into granulosa cells may regulate oocyte meiotic competence during in vitro maturation of mouse follicles

2013 ◽  
Vol 28 (11) ◽  
pp. 3050-3061 ◽  
Author(s):  
Y. J. Kim ◽  
S.-Y. Ku ◽  
Y. Y. Kim ◽  
H. C. Liu ◽  
S. W. Chi ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Meiotic Competence

scholarly journals Sensitivity of the meiotic stage to hyperthermia during in vitro maturation of porcine oocytes

2017 ◽  
Vol 65 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Katsutoshi Nishio ◽  
Mado Yamazaki ◽  
Masayasu Taniguchi ◽  
Kazuhiko Besshi ◽  
Fumio Morita ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
In Vitro Maturation ◽  
Transition Period ◽  
Germinal Vesicle ◽  
Culture Period ◽  
Meiotic Stage ◽  
Meiotic Competence ◽  
Metaphase I

The present study was conducted to clarify whether the meiotic stage of porcine oocytes has the highest sensitivity to hyperthermia during in vitro maturation by evaluating meiotic competence and DNA damage. Oocytes were exposed to 41 °C for 12 h at various intervals during 48 h of maturation culture. When the oocytes were exposed to 41 °C from 12 to 24 h of the maturation culture, the proportion of oocytes reaching metaphase II (MII) decreased as compared to the control oocytes cultured at 38.5 °C (P < 0.05). Moreover, the proportions of DNA fragmentation in all oocytes exposed to 41 °C in each culture period after 12 h from the start of maturation culture were significantly higher (P < 0.05) than for the control oocytes. When the meiotic stage of oocytes cultured at 38.5 °C between 12 and 24 h was examined, the majority of oocytes remained at the germinal vesicle (GV) stage at 12 h and approximately half of the oocytes reached metaphase I (MI) at 24 h. These results indicate that the meiotic stage of porcine oocytes having the highest sensitivity to hyperthermia during in vitro maturation is a transition period from the GV stage to the MI stage.

Granulosa cells affect in vitro maturation and subsequent parthenogenetic development of buffalo ( Bubalus bubalis ) oocytes

2021 ◽  
Author(s):  
Jun Zhang ◽  
Haoxin Wang ◽  
Jiaka Lu ◽  
Qing Yu ◽  
Penghui Fu ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Bubalus Bubalis ◽  
Parthenogenetic Development

In vitro maturation on ovarian granulosa cells encapsulated in agarose matrix improves developmental competence of porcine oocytes

2021 ◽  
Vol 164 ◽  
pp. 42-50
Author(s):  
Ji Eun Park ◽  
Joohyeong Lee ◽  
Seung Tae Lee ◽  
Eunsong Lee
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Developmental Competence ◽  
Ovarian Granulosa Cells

51 Effect of Cryoprotectant Exposure Time on Development of Vitrified-Warmed Immature Equine Oocytes

2018 ◽  
Vol 30 (1) ◽  
pp. 164
Author(s):  
H. S. Canesin ◽  
J. G. Brom-de-Luna ◽  
Y.-H. Choi ◽  
A. M. Pereira ◽  
G. G. Macedo ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Propylene Glycol ◽  
Exposure Time ◽  
In Vitro Maturation ◽  
Initial Exposure ◽  
Exact Test ◽  
Fetal Bovine ◽  
Follicle Aspiration ◽  
Meiotic Competence

Effective methods for cryopreservation of equine oocytes have not yet been established. Vitrification involves use of high cryoprotectant (CPA) concentrations, which can be cytotoxic. Thus, it is critical to determine a CPA concentration and exposure time able to protect the cell during cooling but with a minimal toxicity. Using a rapid non-equilibrating system, we fixed the time in the first, lower CPA concentration solution (V1) at 40 s, based on the time to maximal shrinkage. We then evaluated different exposure times in the final vitrification solution (V2). Cumulus-oocyte complexes (COC) were collected from slaughterhouse-derived ovaries and held overnight in commercial embryo holding medium. Fetal bovine serum was used as the base medium (BM). In experiment 1, COC were held in BM, incubated in V1 (2% propylene glycol + 2% ethylene glycol) for 40 s followed by incubation in V2 (17.5% propylene glycol + 17.5% ethylene glycol + 0.3 M trehalose) for 0, 45, 75, or 110 s, and then loaded in groups of 6 to 10 oocytes on a 75-µm steel mesh and plunged into liquid nitrogen. Warming was performed in decreasing trehalose concentrations in BM: 0.4 M (60-70 s), 0.2 M (5 min), 0.1 M (5 min), 0.05 M (5 min), and 0 M. After warming, oocytes were cultured for in vitro maturation (IVM) and evaluated after staining with Hoechst 33258. Differences between treatments were analysed by Fisher’s exact test. The maturation (metaphase II, MII) rate of the Control (non-vitrified oocytes; 38.8%, 31/80) was similar to that of the 75-s treatment (34.8%, 16/46; P = 0.71), and higher (P < 0.05) than those of the 0, 45, and 110 s treatments (0.0%, 0/10; 11.4%, 4/35; and 3.6%, 1/28; respectively). In experiment 2, timings in V2 focusing around 75 s were evaluated. The COC were collected and vitrified as for experiment 1, except that time in V2 was 50, 60, 70, 80, 90, or 100 s. The vitrified COC were then shipped to the intracytoplasmic sperm injection (ICSI) laboratory. After warming and IVM, oocytes were subjected to ICSI and embryo culture. Control oocytes were recovered by transvaginal follicle aspiration. The MII rate of the Control (60%, 33/55) was similar (P > 0.05) to that of the 60- and 70-s treatments (38.9%, 7/18, and 35.3%, 6/17, respectively), and higher (P < 0.05) than those of the 50-, 80-, 90-, and 100-s treatments (5.6 to 31.6%). The cleavage rates were 94% (31/33) for the Control and 71 to 100% for vitrified oocytes (P > 0.05). No blastocyst was produced from vitrified oocytes compared with 8/33 (24.2%) for Control. This work demonstrates that a rapid, non-equilibrating vitrification technique using a 40-s initial exposure and 70- to 80-s final exposure to CPA is associated with maintenance of meiotic competence of immature equine oocytes; however, further work is required to optimize embryonic development with this method. Research supported by the Clinical Equine ICSI Program and the Link Equine Research Fund, Texas A&M University.

scholarly journals Expanded equine cumulus–oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus–oocyte complexes

2018 ◽  
Vol 30 (2) ◽  
pp. 297 ◽  
Author(s):  
L. González-Fernández ◽  
M. J. Sánchez-Calabuig ◽  
M. G. Alves ◽  
P. F. Oliveira ◽  
S. Macedo ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Granulosa Cells ◽  
Glucose Consumption ◽  
Cell Types ◽  
Solute Carrier Family ◽  
Necrosis Factor Alpha ◽  
Maturation Rate ◽  
Solute Carrier ◽  
Meiotic Competence

Equine cumulus–oocyte complexes (COCs) are classified as compact (cCOC) or expanded (eCOC) and vary in their meiotic competence. This difference could be related to divergent glucose metabolism. To test this hypothesis in the present study, eCOCs, cCOCs and expanded or compact mural granulosa cells (EC and CC respectively) were matured in vitro for 30 h, at which time maturation rate, glucose metabolism and the expression of genes involved in glucose transport, glycolysis, apoptosis and meiotic competence were determined. There were significant differences between eCOCs and cCOCs in maturation rate (50% vs 21.7% (n = 192 and 46) respectively; P < 0.001), as well as mean (± s.e.m.) glucose consumption (1.8 ± 0.5 vs 27.9 ± 5.9 nmol per COC respectively) and pyruvate (0.09 ± 0.01 vs 2.4 ± 0.8 nmol per COC respectively) and lactate (4.7 ± 1.3 vs 64.1 ± 20.6 nmol per COC respectively; P < 0.05 for all) production. Glucose consumption in EC and CC did not differ significantly. Expression of hyaluronan-binding protein (tumour necrosis factor alpha induced protein 6; TNFAIP6) was increased in eCOCs and EC, and solute carrier family 2 member 1 (SLC2A1) expression was increased in eCOCs, but there were no differences in the expression of glycolysis-related enzymes and solute carrier family 2 member 3 (SLC2A3) between the COC or mural granulosa cell types. The findings of the present study demonstrate that metabolic and genomic differences exist between eCOCs and cCOCs and mural granulosa cells in the horse.

Control of in vitro maturation of bovine cumulus-oocyte complex by preovulatory granulosa cells

1993 ◽  
Vol 34 (4) ◽  
pp. 431-442 ◽  
Author(s):  
Flora Rabahi ◽  
Danielle Monniaux ◽  
Claudine Pisselet ◽  
Philippe Durand
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Cumulus Oocyte Complex

scholarly journals In vitro maturation of ovine oocyte in a modified granulosa cells co-culture system and alpha-tocopherol supplementation: effects on nuclear maturation and cleavage

2015 ◽  
Vol 57 (1) ◽  
Author(s):  
Hamideh Adeldust ◽  
Saeed Zeinoaldini ◽  
Hamid Kohram ◽  
Mahmoud Amiri Roudbar ◽  
Morteza Daliri Joupari
Keyword(s):  
Granulosa Cells ◽  
Culture System ◽  
In Vitro Maturation ◽  
Alpha Tocopherol ◽  
Nuclear Maturation

scholarly journals In vitro maturation of bovine granulosa cells for steroid hormone production

2011 ◽  
Vol 60 (232) ◽  
pp. 1331-1334
Author(s):  
O.F. Smith ◽  
A.A. Ogunsola ◽  
A.O. Ladokun ◽  
T.A. Ajadi
Keyword(s):  
Granulosa Cells ◽  
In Vitro Maturation ◽  
Steroid Hormone ◽  
Hormone Production
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