Optic nerve compression in craniopharyngioma by taut anterior cerebral arteries visualized in frontal and transsphenoidal exposures: illustrative cases

BACKGROUND During initial exposure and removal of craniopharyngioma in pediatric patients with severe visual field deficits, the authors have encountered severe deformation of the optic apparatus by taut anterior cerebral arteries as seen during both frontal craniotomy and transsphenoidal exposures. OBSERVATIONS The authors report two pediatric patients with craniopharyngioma whose severe preoperative visual deficits were associated not only with large suprasellar masses but also with severe optic nerve and chiasm compression by taut anterior cerebral arteries. In each patient, the optic nerves were partially cleft by these vessels’ indenting them. LESSONS The role of a taut anterior cerebral artery complex in compression of the optic apparatus in patients with suprasellar tumors has been reported previously, but the intraoperative images in these two cases dramatically reveal this phenomenon.

Pathological Approach to Pleural Malignant Mesothelioma

Exposure to asbestos can lead to asbestosis or malignancy 10-40 years after initial exposure [1]. Although its use has been banned in multiple countries, past occupational exposure leads to most cases that we see in present time. Malignant mesothelioma is an insidious and rare neoplasm that can arise from mesothelial surface cells, being Malignant Pleural Mesothelioma (MPM) the most common type. Lifetime risk of developing mesothelioma among asbestos workers can be as high as 10 percent and latency period is approximately 30-40 years since time of exposure to development of disease [2]. Annual incidence in the united states is approximately 3,300 cases per year [3]. Median overall survival of patients with advanced unresectable disease is approximately 12 months [4]. Clinical suspicion should arise in patients with previous exposure to asbestos who present with pleural thickening and/or effusion with associated respiratory symptoms. Most symptoms are nonspecific such as chest pain, dyspnea, cough and night sweats. Initial evaluation includes chest x-ray, contrast enhanced CT of the chest to find pleural abnormalities, thoracentesis and closed pleural biopsy. However, difficulties establishing diagnosis have been illustrated on studies where thoracentesis and pleural fluid cytology only yields diagnosis in 26% of cases. The diagnosis, then, is established by morphologic and immunohistochemistry findings of cytologic and surgical specimens.

Spreading digital literacy via Minimal English. The concept of ‘class’ in Swift programming language

The article aims at providing explications of the concept of a class, as it is implemented in the Swift programming language offered by Apple. The explications are framed in Minimal English, which is based on the theory of Natural Semantic Metalanguage. Detailed analysis of the Swift concept of class leads to four distinct core explications of the programming construct in question and the related feature that Swift classes possess, namely the concept of property. The article’s primary purpose is to offer a more smooth experience with programming, especially with beginners in mind. Their initial exposure to programming might face several challenges due to the complicated digital jargon of the documentation. Minimal English is implemented to ease the learning curve and promote digital literacy as one of the most fundamental skills in today’s world.

Lessons in French

None of the writers in my study can call French, without hesitation and qualification, a mother tongue. Some of them didn’t start studying the language until they arrived in Paris in their twenties and grappled with learning a new form of expression at a relatively late age. When they recall their initial exposure to this foreign tongue, they describe a fascinating apprenticeship involving dictionaries and renowned works of French literature, and they often shed light on the distinction between oral and written competence in their experience. It is crucial to note that even those authors who have long been fluent in French underscore their non-native relationship to it. Chapter 3 addresses the approaches of these worldwide women writers to French and examines their inventive literary publications in this tongue. It is sensitive to the history of this language and its inextricable connection to a colonial past that many of these writers experienced or became aware of in their homeland. It also focuses on the reality that, for almost all of these authors, this is not the only tongue with which they are familiar. For multilingual individuals, selecting French as their language of literary creation is often the result of a conscious choice motivated by a particular affinity. What comes through in their reflections is most often a passion for this language and a confirmation of the freedom it affords them, as well as an affirmation of its inimitable music that makes it especially well-suited for creative compositions.

Mortality of Fruit Flies (Diptera: Tephritidae) Exposed to Spinetoram Toxic Bait in the Laboratory

Fruit flies (Diptera: Tephritidae) cause significant losses during the production and marketing of horticultural products. Brazilian growers usually adopt full-coverage insecticide spraying to control fruit flies, but toxic bait is a more strategic technique, because reach efficacy and the target surface is the foliage and branches. We provide information regarding the toxicity of spinetoram bait to two fruit fly species in the laboratory as an alternative to organophosphates and the specific spinosad formulation. We tested toxic baits in the laboratory, using commercial hydrolysed corn protein (10% v/v) plus 90 g, 120 g, 150 g and 180 g dilutions of spinetoram 250 WG (commercial product/1,000 litres of water). All toxic baits were compared with an untreated control (only protein) for the adults of females and males of Anastrepha obliqua (Macquart, 1835) and Ceratitis capitata (Wiedemann, 1824) up to 30 hours of exposure. Dry food for adults was included in all dilutions (5% w/v). In addition, we tested the residual effect of toxic baits applied to the leaves of mandarin seedlings. We used the same treatments of the earlier bioassay without dry food, collecting treated leaves and exposing them to C. capitata (medfly) females for 24 hours in the laboratory. Leaves were collected 1, 3, 7, 15 and 30 days after application. Overall, medfly adults were more susceptible to spinetoram baits than A. obliqua. All toxic baits resulted in 100% C. capitata mortality 24 hours after initial exposure, and the toxic bait at 150 g/1,000 L of water resulted in the maximum mortality (96%) in A. obliqua. Except for 90 g of spinetoram bait at 30 days after application, all spinetoram bait concentrations resulted in significantly, more dead C. capitata females than the control over all tested periods in the residual bioassay. At 30 days after application, spinetoram baits at 120 g, 150 g and 180 g resulted in 85%, 87% and 86% mortality in C. capitata, respectively. Spinetoram toxic baits have proven promising for long-term fruit fly management.

Protective Role of an Initial Low-Dose Septic Challenge against Lethal Sepsis in Neonatal Mice: A Pilot Study

Neonatal sepsis is characterized by systemic bacterial invasion followed by a massive inflammatory response. At present, no therapeutic strategy has been found that significantly reduces the mortality of neonatal sepsis. We aimed to investigate the protective role of an initial low-dose septic challenge for the prevention of subsequent lethal sepsis in a mouse model. A stock cecal slurry (CS) solution was prepared from adult ceca. The LD83 (1.5 mg CS/g) was used for all animals. An initial challenge of normal saline (NS) or 0.5 mg CS/g (non-lethal dose) was administered at four days of age, then 1.5 mg CS/g was administered intraperitoneally at seven days of age (72 h post-initial challenge), and survival was monitored. Initial exposure to NS (n = 10) resulted in 90% mortality following exposure to the LD83 CS dose in contrast to an initial exposure to CS (n = 16), which significantly decreased mortality to 6% (p < 0.0001), reduced blood bacterial counts, attenuated inflammatory responses, and suppressed lipid mediators. Initial exposure to a non-lethal CS dose prior to exposure to a lethal CS dose significantly reduces sepsis mortality, a protective effect that might be mediated by modulating abnormal systemic inflammatory responses.

Documentation of the Development of Various Visuomotor Responses in Typically Reared Kittens and Those Reared With Early Selected Visual Exposure by Use of a New Procedure

A new procedure was used to study the development of gaze (responses to moving targets or laser spots in normal kittens, those that had been reared in total darkness to 6 weeks of age, and others that received a period of monocular deprivation (MD). Gaze responses were observed to all stimuli in normal kittens at between 25–30 days of age and striking responses occurred on the same day or the next. Despite slow acquisition of spatial vision in the dark reared kittens over 3 months, they were able to follow and even strike at moving visual stimuli within a day of their initial exposure to light. By contrast, for a week following a period of MD, kittens showed no gaze or striking responses to moving stimuli when using their previously deprived eye. The very different profiles of acquisition of visuomotor skills and spatial vision in visually deprived kittens point to a dissociation between the neuronal populations that support these functions.

Exploring the Shift From HIV Pre-exposure Prophylaxis Awareness to Uptake Among Young Gay and Bisexual Men

Introduction: HIV pre-exposure prophylaxis (PrEP) in the form of a daily oral medication is highly effective at preventing HIV. In the United States, awareness about PrEP has steadily increased over time among individuals vulnerable to HIV, however awareness has not translated into widescale uptake. Estimates are that fewer than 20% of 1.2 million Americans for whom PrEP is indicated are utilizing it. We sought to understand how individuals moved from PrEP awareness to PrEP utilization.Methods: We conducted a series (n = 31) of in-depth interviews with young people, predominantly gay and bisexual men, ages 18–29 years old between February 2015 and January 2016, as part of the evaluation of a multi-year demonstration project funded to test innovative approaches to improve sexual health outcomes and curb the HIV epidemic in California. Interviews were audio-recorded and transcribed verbatim. We conducted a thematic analysis.Results: We present a continuum of PrEP awareness that spans three phases—basic, moderate and advanced. Participants rarely reported becoming well-informed about PrEP over the course of an initial exposure to PrEP information. Learning occurred after multiple exposures to PrEP information through numerous intersecting forms, messengers and formal and informal communication channels. Positively framed messages delivered by formal messengers emphasizing PrEP as a sensible HIV prevention strategy and explicitly communicating a regard for sexual wellness were overwhelmingly persuasive and facilitated movement to the advanced awareness phase. Once participants reached the advanced phase of PrEP awareness, uptake was possible.Conclusions: Our analysis provides insights into how PrEP awareness led to PrEP uptake among young gay and bi-sexual men. Building demand among those in the basic awareness phase took longer than those in the moderate phase. Individuals involved in formal and informal PrEP education can set reasonable expectations about whether, when and how eventual uptake may occur when keeping the continuum of PrEP awareness framework in mind. Many young, gay and bi-sexual male prospective PrEP users will benefit from positively framed messages that emphasize personal well-being, including social, sexual and emotional benefits of PrEP use.

Evolution of anti-modified protein antibody responses can be driven by consecutive exposure to different post-translational modifications

Background Besides anti-citrullinated protein antibodies (ACPA), rheumatoid arthritis patients (RA) often display autoantibody reactivities against other post-translationally modified (PTM) proteins, more specifically carbamylated and acetylated proteins. Immunizing mice with one particular PTM results in an anti-modified protein antibody (AMPA) response recognizing different PTM-antigens. Furthermore, human AMPA, isolated based on their reactivity to one PTM, cross-react with other PTMs. However, it is unclear whether the AMPA-reactivity profile is “fixed” in time or whether consecutive exposure to different PTMs can shape the evolving AMPA response towards a particular PTM. Methods Longitudinally collected serum samples of 8 human individuals at risk of RA and 5 with early RA were tested with ELISA, and titers were analyzed to investigate the evolution of the AMPA responses over time. Mice (13 per immunization group in total) were immunized with acetylated (or carbamylated) protein (ovalbumin) twice or cross-immunized with an acetylated and then a carbamylated protein (or vice versa) and their serum was analyzed for AMPA responses. Results Human data illustrated dynamic changes in AMPA-reactivity profiles in both individuals at risk of RA and in early RA patients. Mice immunized with either solely acetylated or carbamylated ovalbumin (AcOVA or CaOVA) developed reactivity against both acetylated and carbamylated antigens. Irrespective of the PTM-antigen used for the first immunization, a booster immunization with an antigen bearing the other PTM resulted in increased titers to the second/booster PTM. Furthermore, cross-immunization skewed the overall AMPA-response profile towards a relatively higher reactivity against the “booster” PTM. Conclusions The relationship between different reactivities within the AMPA response is dynamic. The initial exposure to a PTM-antigen induces cross-reactive responses that can be boosted by an antigen bearing this or other PTMs, indicating the formation of cross-reactive immunological memory. Upon subsequent exposure to an antigen bearing another type of PTM, the overall reactivity pattern can be skewed towards better recognition of the later encountered PTM. These data might explain temporal differences in the AMPA-response profile and point to the possibility that the PTM responsible for the initiation of the AMPA response may differ from the PTM predominantly recognized later in time.

An Investigation on the Process of Reimbibition of Oil and Its Impact on Oil Recovery in the Yates Field

Summary In mixed- to oil-wet reservoirs characterized by intense natural fracturing where the dominant displacement mechanism is gravity drainage, surfactant injection can lead to a shift in wettability and incremental oil production. In some cases, oil can also reimbibe back into the rock matrix after the oil saturation has been reduced upon initial exposure to surfactant, suggesting limited permanence in the wettability shift. The reimbibition phenomenon is investigated in this paper using Amott cells. Three cationic surfactants (C12-, C12–16-, C16-based) with interfacial tensions (IFT) between 0.18 and 0.95 mN/m were preselected to be evaluated. Current application of the C12-based surfactant in the Yates field is considered successful based on incremental oil recovery seen during the treatment. Silurian dolomite (SD) rock samples were flooded with Yates crude oil before being aged at 60°C for 6 weeks. For the imbibition tests, the aqueous surfactant solution was set as the external phase within the Amott cell, and the recovery of oil was recorded periodically. After the imbibition tests ended, the rock samples were placed in an inverse Amott cell with the Yates oil as the external phase. Baseline tests were first conducted to show that without a surfactant in the oil or brine, no imbibition occurred. With a surfactant concentration of 3,000 ppm, oil recovery at the end of the imbibition tests varied from 34 to 60% of the original oil volume in the core sample. During the reimbibition test, a large amount of oil was able to reimbibe into the rock, displacing the brine. Most of the displacement occurred within the first 2 weeks. The net oil recovery, taken as the final volume of oil recovered in the imbibition test minus the final volume of oil reimbibed into the rock, ranged from 0 to 18%. Given the possibility of surfactant dilution in field applications, another set of tests was conducted with 1,500 ppm. A reduction in oil recovery during imbibition was observed for all the tested surfactants. Partition coefficients were determined for each of the tested surfactants, and the ion-pair mechanism was used to explain the net oil recovery results. Lastly, the impact of rock permeability on reimbibition was investigated. Results show increasing permeability may lead to a linear response in oil reimbibition; therefore, minimizing the permeability range when selecting rock samples may be necessary when conducting the reimbibition test. The importance of oil reimbibition is demonstrated in the experimental study, and we make an argument for conducting both the imbibition and reimbibition tests to better evaluate surfactant efficacy. The improved understanding of wettability alteration should lead to advancements in chemical enhanced oil recovery (EOR) designs for field treatments.