The Brassica Mature Pollen and Stigma Proteomes: Preparing to Meet

2021 ◽  
Author(s):  
Reneé Robinson ◽  
Vishwanath Sollapura ◽  
Philippe Couroux ◽  
Dave Sprott ◽  
Michael Ravensdale ◽  
...  
Keyword(s):  
Mature Pollen

scholarly journals A Rapid Pipeline for Pollen- and Anther-Specific Gene Discovery Based on Transcriptome Profiling Analysis of Maize Tissues

2021 ◽  
Vol 22 (13) ◽  
pp. 6877
Author(s):  
Yannan Shi ◽  
Yao Li ◽  
Yongchao Guo ◽  
Eli James Borrego ◽  
Zhengyi Wei ◽  
...  
Keyword(s):  
Seed Production ◽  
Late Stage ◽  
Mature Pollen ◽  
Hybrid Breeding ◽  
Large Set ◽  
Male Sterile ◽  
Reproductive Process ◽  
Maize Pollen ◽  
Cis Element ◽  
Mature Anther

Recently, crop breeders have widely adopted a new biotechnology-based process, termed Seed Production Technology (SPT), to produce hybrid varieties. The SPT does not produce nuclear male-sterile lines, and instead utilizes transgenic SPT maintainer lines to pollinate male-sterile plants for propagation of nuclear-recessive male-sterile lines. A late-stage pollen-specific promoter is an essential component of the pollen-inactivating cassette used by the SPT maintainers. While a number of plant pollen-specific promoters have been reported so far, their usefulness in SPT has remained limited. To increase the repertoire of pollen-specific promoters for the maize community, we conducted a comprehensive comparative analysis of transcriptome profiles of mature pollen and mature anthers against other tissue types. We found that maize pollen has much less expressed genes (>1 FPKM) than other tissue types, but the pollen grain has a large set of distinct genes, called pollen-specific genes, which are exclusively or much higher (100 folds) expressed in pollen than other tissue types. Utilizing transcript abundance and correlation coefficient analysis, 1215 mature pollen-specific (MPS) genes and 1009 mature anther-specific (MAS) genes were identified in B73 transcriptome. These two gene sets had similar GO term and KEGG pathway enrichment patterns, indicating that their members share similar functions in the maize reproductive process. Of the genes, 623 were shared between the two sets, called mature anther- and pollen-specific (MAPS) genes, which represent the late-stage pollen-specific genes of the maize genome. Functional annotation analysis of MAPS showed that 447 MAPS genes (71.7% of MAPS) belonged to genes encoding pollen allergen protein. Their 2-kb promoters were analyzed for cis-element enrichment and six well-known pollen-specific cis-elements (AGAAA, TCCACCA, TGTGGTT, [TA]AAAG, AAATGA, and TTTCT) were found highly enriched in the promoters of MAPS. Interestingly, JA-responsive cis-element GCC box (GCCGCC) and ABA-responsive cis-element-coupling element1 (ABRE-CE1, CCACC) were also found enriched in the MAPS promoters, indicating that JA and ABA signaling likely regulate pollen-specific MAPS expression. This study describes a robust and straightforward pipeline to discover pollen-specific promotes from publicly available data while providing maize breeders and the maize industry a number of late-stage (mature) pollen-specific promoters for use in SPT for hybrid breeding and seed production.

scholarly journals Storage substances in the androgametogenesis and mature pollen grain of Ilex paraguariensis St. Hil. (Aquifoliaceae)

1999 ◽  
Vol 22 (2) ◽  
pp. 125-131 ◽  
Author(s):  
RINALDO PIRES DOS SANTOS ◽  
JORGE ERNESTO DE ARAUJO MARIATH
Keyword(s):  
Pollen Grain ◽  
Mature Pollen ◽  
Ilex Paraguariensis

scholarly journals Transformation of Seed Non-Transmissible Hop Viroids in Nicotiana benthamiana Causes Distortions in Male Gametophyte Development

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2398
Author(s):  
Lenka Steinbachová ◽  
Jaroslav Matoušek ◽  
Gerhard Steger ◽  
Helena Matoušková ◽  
Sebastjan Radišek ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Male Gametophyte ◽  
Pollen Grains ◽  
Potato Spindle Tuber Viroid ◽  
Apple Fruit ◽  
Mature Pollen ◽  
Rare Occurrence ◽  
Gametophyte Development ◽  
Male Gametophyte Development ◽  
Marker Proteins

Viroids are small, non-coding, parasitic RNAs that promote developmental distortions in sensitive plants. We analyzed pollen of Nicotiana benthamiana after infection and/or ectopic transformation with cDNAs of citrus bark cracking viroid (CBCVd), apple fruit crinkle viroid (AFCVd) and potato spindle tuber viroid (PSTVd) variant AS1. These viroids were seed non-transmissible in N. benthamiana. All viroids propagated to high levels in immature anthers similar to leaves, while their levels were drastically reduced by approximately 3.6 × 103, 800 and 59 times in mature pollen of CBCVd, AFCVd and PSTVd infected N. benthamiana, respectively, in comparison to leaves. These results suggest similar elimination processes during male gametophyte development as in the Nicotiana tabacum we presented in our previous study. Mature pollen of N. benthamiana showed no apparent defects in infected plants although all three viroids induced strong pathological symptoms on leaves. While Nicotiana species have naturally bicellular mature pollen, we noted a rare occurrence of mature pollen with three nuclei in CBCVd-infected N. benthamiana. Changes in the expression of ribosomal marker proteins in AFCVd-infected pollen were detected, suggesting some changes in pollen metabolism. N. benthamiana transformed with 35S-driven viroid cDNAs showed strong symptoms including defects in pollen development. A large number of aborted pollen (34% and 62%) and a slight increase of young pollen grains (8% and 15%) were found in mature pollen of AFCVd and CBCVd transformants, respectively, in comparison to control plants (3.9% aborted pollen and 0.3% young pollen). Moreover, pollen grains with malformed nuclei or trinuclear pollen were found in CBCVd-transformed plants. Our results suggest that “forcing” overexpression of seed non-transmissible viroid led to strong pollen pathogenesis. Viroid adaptation to pollen metabolism can be assumed as an important factor for viroid transmissibility through pollen and seeds.

scholarly journals Liquid Culture System for Mature Pollen in Hyuganatsu (Citrus tamurana hort. ex Tanaka)

2012 ◽  
Vol 11 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Asuka Uchida ◽  
Akiha Abe ◽  
Yoichiro Hoshino ◽  
Hisato Kunitake
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Mature Pollen ◽  
Liquid Culture System

scholarly journals On the Mature Pollen Grains in Angiosperms

1937 ◽  
Vol 51 (606) ◽  
pp. 524-529 ◽  
Author(s):  
Nobuhide Suita
Keyword(s):  
Pollen Grains ◽  
Mature Pollen

Changes in the Plantago lanceolata mature pollen ultrastructure and Pla l 1 allergen localisation after grain hydration

Grana ◽  
2019 ◽  
Vol 58 (6) ◽  
pp. 447-461
Author(s):  
Zulima González-Parrado ◽  
Delia Fernández-González ◽  
Rosa M. Valencia-Barrera ◽  
Ana M. Vega-Maray
Keyword(s):  
Plantago Lanceolata ◽  
Mature Pollen ◽  
Pollen Ultrastructure

Isolation of sperm cells from mature pollen grains of Spinacia oleracea L.

1989 ◽  
Vol 2 (2) ◽  
pp. 97-102 ◽  
Author(s):  
C. H. Theunis ◽  
J. L. Van Went
Keyword(s):  
Spinacia Oleracea ◽  
Pollen Grains ◽  
Mature Pollen ◽  
Sperm Cells ◽  
Spinacia Oleracea L

scholarly journals Conserved Expression of the Arabidopsis ACT1 and ACT3 Actin Subclass in Organ Primordia and Mature Pollen

1996 ◽  
Vol 8 (1) ◽  
pp. 15
Author(s):  
Yong-Qiang An ◽  
Shurong Huang ◽  
John M. McDowell ◽  
Elizabeth C. McKinney ◽  
Richard B. Meagher
Keyword(s):  
Mature Pollen

scholarly journals Unique actin and microtubule arrays co-ordinate the differentiation of microspores to mature pollen in Nicotiana tabacum

1999 ◽  
Vol 50 (334) ◽  
pp. 581-594 ◽  
Author(s):  
L. Zonia ◽  
J. Tupy ◽  
C. J. Staiger
Keyword(s):  
Nicotiana Tabacum ◽  
Mature Pollen
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