The Arabidopsis electron‐transfer flavoprotein:ubiquinone oxidoreductase is required during normal seed development and germination

2021 ◽  
Author(s):  
Paula Fonseca‐Pereira ◽  
Phuong Anh Pham ◽  
João Henrique F. Cavalcanti ◽  
Rebeca P. Omena‐Garcia ◽  
Jessica A. S. Barros ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Seed Development ◽  
Normal Seed

scholarly journals SDG711 Is Involved in Rice Seed Development through Regulation of Starch Metabolism Gene Expression in Coordination with Other Histone Modifications

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Xiaoyun Liu ◽  
Junling Luo ◽  
Tiantian Li ◽  
Huilan Yang ◽  
Ping Wang ◽  
...  
Keyword(s):  
Seed Development ◽  
Starch Synthesis ◽  
Endosperm Development ◽  
Starch Accumulation ◽  
Body Region ◽  
Rice Seed ◽  
Normal Seed ◽  
Gene Body Region ◽  
Metabolism Gene

AbstractSDG711 is a histone H3K27me2/3 transmethylase in rice, a homolog of CLF in Arabidopsis, and plays key roles in regulating flowering time and panicle development. In this work, we investigated the role of SDG711 in rice seed development. Overexpression and downregulation of SDG711 lead to a decrease and increase in the expression level of genes related to starch accumulation, resulting in smaller seeds or even seed abortion. ChIP assay showed that SDG711-mediated H3K27me3 changed significantly in genes related to endosperm development, and SDG711 can directly bind to the gene body region of several starch synthesis genes and amylase genes. In addition, H3K4me3 and H3K9ac modifications also cooperate with H3K27me3 to regulate the development of the endosperm. Our results suggest that the crosstalk between SDG711-mediated H3K27me3 and H3K4me3, and H3K9ac are involved in starch accumulation to control normal seed development.

scholarly journals The Arabidopsis Group 1 LATE EMBRYOGENESIS ABUNDANT Protein ATEM6 Is Required for Normal Seed Development

2005 ◽  
Vol 140 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Alicia J. Manfre ◽  
Lea M. Lanni ◽  
William R. Marcotte
Keyword(s):  
Seed Development ◽  
Late Embryogenesis Abundant ◽  
Late Embryogenesis Abundant Protein ◽  
Abundant Protein ◽  
Late Embryogenesis ◽  
Normal Seed ◽  
Group 1

scholarly journals SDG711 is Involved in Rice Seed Development Through Regulation of Starch Metabolism Gene Expression in Coordination With Other Histone Modifications

2020 ◽  
Author(s):  
Xiaoyun Liu ◽  
Junling Luo ◽  
Tiantian Li ◽  
Huilan Yang ◽  
Ping Wang ◽  
...  
Keyword(s):  
Seed Development ◽  
Starch Synthesis ◽  
Endosperm Development ◽  
Starch Accumulation ◽  
Body Region ◽  
Rice Seed ◽  
Normal Seed ◽  
Gene Body Region ◽  
Metabolism Gene

Abstract SDG711 is a histone H3K27me2/3 transmethylases in rice, homolog ofCLFin Arabidopsis, that plays key roles in regulating of flowering time and panicle development. In this work, we investigated that the role of SDG711 in rice seed development. Overexpression and down-regulation of SDG711lead to the decrease and increase of the expression level of genes related to starch accumulation, resulting in smaller seed or even seed abortion. ChIP assay showed that SDG711-mediated H3K27me3 changed significantly in genes related to endosperm development and SDG711 can directly bind to the gene body region of several starch synthesis genes and amylase genes. In addition, H3K4me3 and H3K9ac modifications also cooperate with H3K27me3 to regulate the development of endosperm. Our results suggested that the crosstalk of SDG711-mediated H3K27me3 with H3K4me3 and H3K9ac are involved in starch accumulation to control normal seed development.

Control of Wild Oats by Prevention of Normal Seed Development with Sodium 2,2-Dichloropropionate

Weeds ◽  
1958 ◽  
Vol 6 (3) ◽  
pp. 263 ◽  
Author(s):  
Robert N. Andersen ◽  
E. A. Helgeson
Keyword(s):  
Seed Development ◽  
Wild Oats ◽  
Normal Seed

A light optical diffractometer for electron microscopical images operating in line

1978 ◽  
Vol 36 (1) ◽  
pp. 86-87
Author(s):  
P. Bonhomme ◽  
A. Beorchia
Keyword(s):  
Electron Microscopy ◽  
Electron Transfer ◽  
Electron Microscope ◽  
Image Converter ◽  
Coherent Light ◽  
Spatial Filters ◽  
Electron Image ◽  
Electron Microscopical ◽  
Working Parameters ◽  
Amorphous Part

We have already described (1.2.3) a device using a pockel's effect light valve as a microscopical electron image converter. This converter can be read out with incoherent or coherent light. In the last case we can set in line with the converter an optical diffractometer. Now, electron microscopy developments have pointed out different advantages of diffractometry. Indeed diffractogram of an image of a thin amorphous part of a specimen gives information about electron transfer function and a single look at a diffractogram informs on focus, drift, residual astigmatism, and after standardizing, on periods resolved (4.5.6). These informations are obvious from diffractogram but are usualy obtained from a micrograph, so that a correction of electron microscope parameters cannot be realized before recording the micrograph. Diffractometer allows also processing of images by setting spatial filters in diffractogram plane (7) or by reconstruction of Fraunhofer image (8). Using Electrotitus read out with coherent light and fitted to a diffractometer; all these possibilities may be realized in pseudoreal time, so that working parameters may be optimally adjusted before recording a micrograph or before processing an image.

Biological electron-transfer reactions

1999 ◽  
Vol 34 ◽  
pp. 101-124 ◽  
Author(s):  
A. Grant Mauk
Keyword(s):  
Electron Transfer ◽  
Transfer Reactions ◽  
Electron Transfer Reactions ◽  
Biological Electron Transfer

Flavin radicals, conformational sampling and robust design principles in interprotein electron transfer: the trimethylamine dehydrogenase-electron-transferring flavoprotein complex

2004 ◽  
Vol 71 ◽  
pp. 1-14
Author(s):  
David Leys ◽  
Jaswir Basran ◽  
François Talfournier ◽  
Kamaldeep K. Chohan ◽  
Andrew W. Munro ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Kinetic Studies ◽  
Molecular Assembly ◽  
Conformational Sampling ◽  
Trimethylamine Dehydrogenase ◽  
Key Residues ◽  
Electron Transferring Flavoprotein ◽  
Electron Transfer Complex

TMADH (trimethylamine dehydrogenase) is a complex iron-sulphur flavoprotein that forms a soluble electron-transfer complex with ETF (electron-transferring flavoprotein). The mechanism of electron transfer between TMADH and ETF has been studied using stopped-flow kinetic and mutagenesis methods, and more recently by X-ray crystallography. Potentiometric methods have also been used to identify key residues involved in the stabilization of the flavin radical semiquinone species in ETF. These studies have demonstrated a key role for 'conformational sampling' in the electron-transfer complex, facilitated by two-site contact of ETF with TMADH. Exploration of three-dimensional space in the complex allows the FAD of ETF to find conformations compatible with enhanced electronic coupling with the 4Fe-4S centre of TMADH. This mechanism of electron transfer provides for a more robust and accessible design principle for interprotein electron transfer compared with simpler models that invoke the collision of redox partners followed by electron transfer. The structure of the TMADH-ETF complex confirms the role of key residues in electron transfer and molecular assembly, originally suggested from detailed kinetic studies in wild-type and mutant complexes, and from molecular modelling.

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