Rapid genotyping of 508G > A (rs3745635) and 1067T > A (rs3894326) of FUT3 by a duplex Eprobe‐mediated melting curve analysis

Vox Sanguinis ◽  
2022 ◽  
Author(s):  
Mikiko Soejima ◽  
Yoshiro Koda
Keyword(s):  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Rapid genotypic detection ofBacillus anthracisand theBacillus cereusgroup by multiplex real-time PCR melting curve analysis

2005 ◽  
Vol 43 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Kijeong Kim ◽  
Juwon Seo ◽  
Katherine Wheeler ◽  
Chulmin Park ◽  
Daewhan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Diagnosis of α-1-Antitrypsin Deficiency: An Algorithm of Quantification, Genotyping, and Phenotyping

2006 ◽  
Vol 52 (12) ◽  
pp. 2236-2242 ◽  
Author(s):  
Melissa R Snyder ◽  
Jerry A Katzmann ◽  
Malinda L Butz ◽  
Ping Yang ◽  
D Brian Dawson ◽  
...  
Keyword(s):  
Laboratory Testing ◽  
Null Allele ◽  
Melting Curve ◽  
Curve Analysis ◽  
Laboratory Evaluation ◽  
Melting Curve Analysis ◽  
Genotype Assay ◽  
Antitrypsin Deficiency ◽  
Phenotype Analysis ◽  
A1at Deficiency

Abstract Background: Laboratory testing in suspected α-1-antitrypsin (A1AT) deficiency involves analysis of A1AT concentrations and identification of specific alleles by genotyping or phenotyping. The purpose of this study was to define and evaluate a strategy that provides reliable laboratory evaluation of A1AT deficiency. Methods: Samples from 512 individuals referred for A1AT phenotype analysis were analyzed by quantification, phenotype, and genotype. A1AT concentrations were measured by nephelometry. Phenotype analysis was performed by isoelectric focusing electrophoresis. The genotype assay detected the S and Z deficiency alleles by a melting curve analysis. Results: Of the 512 samples analyzed, 2% of the phenotype and genotype results were discordant. Among these 10 discordant results, 7 were attributed to phenotyping errors. On the basis of these data we formulated an algorithm, according to which we analyzed samples by genotyping and quantification assays, with a reflex to phenotyping when the genotype and quantification results were not concordant. Retrospective analyses demonstrated that 4% of samples submitted for genotype and quantitative analysis were reflexed to phenotyping. Of the reflexed samples, phenotyping confirmed the genotype result in 85% of cases. In the remaining 15%, phenotyping provided further information, including identifying rare deficiency alleles and suggesting the presence of a null allele, and allowed for a more definitive interpretation of the genotype result. Conclusions: The combination of genotyping and quantification, with a reflex to phenotyping, is the optimal strategy for the laboratory evaluation of A1AT deficiency.

Abstract 579: Multiplex digital PCR combined with melting curve analysis for detectingKRASmutations towards pancreatic cancer diagnosis

2021 ◽  
Author(s):  
Junko Tanaka ◽  
Tatsuo Nakagawa ◽  
Takeshi Ishida ◽  
Yoshio Kamura ◽  
Akiko Shiratori ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Diagnosis ◽  
Melting Curve ◽  
Digital Pcr ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Cost-effective and fast KIR gene-content genotyping by multiplex melting curve analysis

HLA ◽  
2018 ◽  
Vol 92 (6) ◽  
pp. 384-391
Author(s):  
Leonardo M. Amorim ◽  
Tiago H. S. Santos ◽  
Jill A. Hollenbach ◽  
Paul J. Norman ◽  
Wesley M. Marin ◽  
...  
Keyword(s):  
Melting Curve ◽  
Cost Effective ◽  
Curve Analysis ◽  
Gene Content ◽  
Melting Curve Analysis ◽  
Kir Gene

Ultrafast real-time PCR with integrated melting curve analysis and duplex capacities using a low-cost polymer lab-on-a-chip system

2015 ◽  
Author(s):  
Rainer Gransee ◽  
Tristan Schneider ◽  
Deniz Elyorgun ◽  
Xenia Strobach ◽  
Tobias Schunck ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Low Cost ◽  
Melting Curve ◽  
Lab On A Chip ◽  
Curve Analysis ◽  
Melting Curve Analysis
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