Ultrafast real-time PCR with integrated melting curve analysis and duplex capacities using a low-cost polymer lab-on-a-chip system

2015 ◽  
Author(s):  
Rainer Gransee ◽  
Tristan Schneider ◽  
Deniz Elyorgun ◽  
Xenia Strobach ◽  
Tobias Schunck ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Low Cost ◽  
Melting Curve ◽  
Lab On A Chip ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Detection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis

2021 ◽  
Vol 51 (9) ◽  
Author(s):  
María Teresa Federici Rodriguez ◽  
Rody Artigas ◽  
Sofía Guerra ◽  
Andrea Branda Sica ◽  
Noelia Vázquez ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Stop Codon ◽  
Low Cost ◽  
Melting Curve ◽  
Premature Stop Codon ◽  
Curve Analysis ◽  
Assay Method ◽  
Melting Curve Analysis ◽  
Group I

ABSTRACT: Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.

scholarly journals Rapid genotypic detection ofBacillus anthracisand theBacillus cereusgroup by multiplex real-time PCR melting curve analysis

2005 ◽  
Vol 43 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Kijeong Kim ◽  
Juwon Seo ◽  
Katherine Wheeler ◽  
Chulmin Park ◽  
Daewhan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

Determination of Helicobacter pylori cagA, vacA genotypes with real-time PCR melting curve analysis

2001 ◽  
Vol 95 (1-6) ◽  
pp. 369-377 ◽  
Author(s):  
Agnes Ruzsovics ◽  
Bela Molnar ◽  
Zsuzsa Unger ◽  
Zsolt Tulassay ◽  
Laszlo Pronai
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Rapid plasmid replicon typing by real time PCR melting curve analysis

2013 ◽  
Vol 13 (1) ◽  
pp. 83 ◽  
Author(s):  
Maikel Boot ◽  
Susanne Raadsen ◽  
Paul HM Savelkoul ◽  
Christina Vandenbroucke-Grauls
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Plasmid Replicon

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection

2021 ◽  
Vol 338 ◽  
pp. 127932
Author(s):  
Jiapeng Li ◽  
Yixuan Wei ◽  
Jinchun Li ◽  
Ruixi Liu ◽  
Suigen Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Sybr Green ◽  
Melting Curve Analysis ◽  
Analysis Method

Identification of eleven meat species in foodstuff by a hexaplex real-time PCR with melting curve analysis

Food Control ◽  
2021 ◽  
Vol 121 ◽  
pp. 107599
Author(s):  
Jinchun Li ◽  
Jiapeng Li ◽  
Ruixi Liu ◽  
Yixuan Wei ◽  
Shouwei Wang
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Meat Species
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