DETERMINATION OF LACTIC ACID PRODUCTION BY RHIZOPUS ORYZAE IN SOLID STATE FERMENTATION OF PINEAPPLE WASTE

Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in tropic region. This study is looking into the potential of utilising solid pineapple waste in solid state fermentation for the production of lactic acid by Rhizopus oryzae. A 2-level factorial design was employed to screen the effect of moisture content (60% and 80%), inoculum size (1×104 spores/g and 1×108 spores/g), pH (4.5 and 6.5), temperature (27°C and 40°C) and particle size (<0.5 mm and >3.15 mm) to the production of lactic acid. The predicted maximum production is 0.0221 g lactic acid/g SPW in SSF condition of 80% moisture; pH 6.5; 1×104 spores/g of inoculum; waste particle of 3.15 mm; and temperature 27°C. Analysis of variance (ANOVA) showed that the model is significant with high value of predicted (0.9616) and adjusted (0.9726) R-squared, indicated a good agreement between the predicted and actual values at each point of the experiment. Post-statistical experiment confirmed the ability of lactic acid production by R. oryzae at the predicted conditions with 0.0236 g lactic acid/g SPW being produced.

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L(+)-Lactic Acid Production Using Lactobacillus Casei in Solid-State Fermentation

Biotechnology Letters ◽

10.1007/s10529-005-2731-8 ◽

2005 ◽

Vol 27 (21) ◽

pp. 1685-1688 ◽

Cited By ~ 43

Author(s):

Pappy John Rojan ◽

Kesavan Madhavan Nampoothiri ◽

Athira Syamaprasad Nair ◽

Ashok Pandey

Keyword(s):

Lactic Acid ◽

Solid State ◽

Solid State Fermentation ◽

Acid Production ◽

Lactobacillus Casei ◽

Lactic Acid Production

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Optimization of L(+) Lactic Acid Production from Solid Pineapple Waste (SPW) by Rhizopus oryzae NRRL 395

Journal of Polymers and the Environment ◽

10.1007/s10924-020-01862-0 ◽

2020 ◽

Cited By ~ 1

Author(s):

Nor Azimah Mohd Zain ◽

Siti Nurbalqis Aziman ◽

Mohd Suardi Suhaimi ◽

Ani Idris

Keyword(s):

Lactic Acid ◽

Acid Production ◽

Rhizopus Oryzae ◽

Lactic Acid Production ◽

Pineapple Waste

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Screening of Lactic Acid Fermentation by Rhizopus oryzae on Banana Peel Using Two-Level Factorial Analysis

Science Letters ◽

10.24191/sl.v16i1.13651 ◽

2022 ◽

Vol 16 (1) ◽

pp. 12

Author(s):

Nor Atikah Husna Ahmad Nasir ◽

Nurul Syafiqah Mohd Yaminudin ◽

Atikah Kamaludin ◽

Sharir Aizat Kamaruddin ◽

Siti Nurbalqis Aziman

Keyword(s):

Lactic Acid ◽

Moisture Content ◽

Acid Production ◽

Rhizopus Oryzae ◽

Lactic Acid Production ◽

Inoculum Size ◽

Factorial Analysis ◽

Banana Peel ◽

Acid Fermentation ◽

Significant Difference

For ages, pure sugars or edible crops have produced lactic acid. However, a major concern on lactic acid production lies in the cost of the raw materials used. Thus, an alternative to overcome this situation is urgently needed. Characterization of banana peels shows that it contains promising sugar that can be utilized for lactic acid production, which are xylose (0.774 g/L), glucose (0.756 g/L) and fructose (0.532 g/L). Thus, this study aims to screen the potential of banana peel as a substrate by using Rhizopus oryzae through batch fermentation for lactic acid production, as R. oryzae can synthesize lactic acid in low nutrient requirements. Two-level factorial analysis was designed to screen the effects of moisture content (60% and 80%), temperature (27 °C and 40 °C), pH (4.5 and 6.5) and inoculum size (1x104 spores/mL and 1x108 spores/g) on the lactic acid production. Based on the Two-level factorial (2LF) analysis, the highest lactic acid production of 0.0813 g/L was detected at 80 % moisture content, pH 4.5, the temperature of 27 °C and inoculum size of 1x104 spores/g. The findings show that most of the conditions have a significant difference between each other (p<0.05). Therefore, the fermentation of banana peels by R. oryzae could be a promising method to produce a lactic acid concentration.

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