DNA BARCODING FOR IDENTIFICATION OF PROCESSED TUNA FISH IN INDONESIAN MARKET

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Mala Nurilmala ◽  
Utut Widyastuti ◽  
Wisnu Ananta Kusuma ◽  
Nurjanaha Nurjanaha ◽  
Nuring Wulansari ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Cytochrome B ◽  
Mitochondrial Gene ◽  
Free Trade Area ◽  
Accurate Method ◽  
Molecular Technique ◽  
Direct Sequence ◽  
Tuna Fish ◽  
Trade Area ◽  
Canned Tuna

DNA barcoding is a molecular technique to characterize species organism using a short DNA sequence. Recently, it becomes useful tool to detect seafood mislabeling and species substitution. Cytochrome b is one of the mitochondrial gene used in DNA barcoding. In order to face the regulation of AFTA (ASEAN Free Trade Area), the accurate method to detect the fish species and its products is needed in order to avoid the fraudulent in Indonesia. Thus, an attempt was carried out to identify authentication for tuna’s products (sushi, fish ball, meat floss, and canned tuna). The samples were collected from, Bogor, West Java, Indonesia. DNA was isolated according to the manufacturer’s protocol. The amplification of DNA by PCR was carried out, then the direct sequence was performed. In the present study, DNA barcoding of tuna’s product using cytochrome b were elucidated. The amplification of DNA by PCR was successfully obtained from tuna’s sample except one of canned tuna (K3). It showed that one of the canned tuna as in the label did not contain tuna. It indicated that there was an economic fraud for one of canned tuna.

Discrimination of Cricotopus species (Diptera: Chironomidae) by DNA barcoding

2008 ◽  
Vol 98 (6) ◽  
pp. 555-563 ◽  
Author(s):  
C.S. Sinclair ◽  
S.E. Gresens
Keyword(s):  
Water Quality ◽  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Mitochondrial Gene ◽  
Accurate Method ◽  
Taxonomic Resolution ◽  
Molecular Approaches ◽  
Putative Species ◽  
Sequence Variations ◽  
Wide Range

AbstractChironomids (Diptera) typically comprise the most abundant group of macroinvertebrates collected in water quality surveys. Species in the genus Cricotopus display a wide range of tolerance for manmade pollutants, making them excellent bioindicators. Unfortunately, the usefulness of Cricotopus is overshadowed by the difficulty of accurately identifying larvae using current morphological keys. Molecular approaches are now being used for identification and taxonomic resolution in many animal taxa. In this study, a sequence-based approach for the mitochondrial gene, cytochrome oxidase I (COI), was developed to facilitate identification of Cricotopus species collected from Baltimore area streams. Using unique COI sequence variations, we developed profiles for seven described Cricotopus sp., four described Orthocladius sp., one described Paratrichocladius sp. and one putative species of Cricotopus. In addition to providing an accurate method for identification of Cricotopus, this method will make a useful contribution to the development of keys for Nearctic Cricotopus.

scholarly journals Review: Progress and potential of DNA barcoding for species identification of fish species

2017 ◽  
Vol 18 (4) ◽  
pp. 1394-1405
Author(s):  
AYESHA IMTIAZ ◽  
SITI AZIZAH MOHD NOR ◽  
DARLINA MD. NAIM
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Fish Species ◽  
Mitochondrial Gene ◽  
Biological Species ◽  
Molecular Technique ◽  
Base Pairs ◽  
Main Challenge ◽  
The Status ◽  
Group A

Imtiaz A, Mohd Nor SA, Md. Naim D. 2017. Review: Progress and potential of DNA barcoding for species identification of fish species. Biodiversitas 18: 1394-1405. DNA barcoding is a molecular technique to identify species by utilizing 600-800 base pairs genetic primer segments of mitochondrial gene cytochrome oxidase I. DNA barcoding has high potential to identify species into taxa, resolves ambiguousness in species identification, helps in accurate species identification, categorize species for conservation and also communize the information in the form of database system. The main challenge to this technique is regarding the use of barcoding information on ‘biological species concept’. The extreme diversity of fish and their economic importance has made this group a major target of DNA barcoding. DNA barcoding can assign the status of known to unknown sample but it also has the ability to detect previously un-sampled species as distinct. In this review, we present an overview of DNA barcoding and introduce current advances and limitation of this promising technique.

Trade Creation or Trade Diversion in ASEAN and ASEAN+6 FTAs: Trade Indicators Approach

2015 ◽  
pp. 25-41
Author(s):  
Anh Tu Thuy ◽  
Ngoc Le Minh
Keyword(s):  
Comparative Advantage ◽  
Wheat Gluten ◽  
Free Trade Area ◽  
Private Investment ◽  
Trade Diversion ◽  
Trade Creation ◽  
Public And Private ◽  
Trade Area ◽  
Economic Partnership ◽  
Regional Comprehensive Economic Partnership

This paper makes use of two trade indicators, Revealed Comparative Advantage (RCA) and Regional Orientation (RO), to evaluate the economic impacts of the ASEAN Free Trade Area (The) and the Regional Comprehensive Economic Partnership (RCEP) on Vietnamese commodities at the Harmonized System (HS) 2-digit level. Several sectors in which Vietnam has revealed a comparative advantage, has benefited from the AFTA, and would continue to enjoy trade creation from the RCEP, are: Cereals (10), Salt, sulphur, earth, stone, plaster, lime and cement (25), Rubber (40), Knitted or crocheted fabric (60), etc. More importantly, the result provides a list of commodities in which Vietnam has a comparative advantage and only experiences trade creation when participating in the RCEP. These are: Milling products, malt, starches, inulin, wheat gluten (11), Vegetable plaiting materials, vegetable products not elsewhere specified (14), Wood and articles of wood, wood charcoal (44), etc. Findings also show commodities in which Vietnam has a comparative advantage; but are not well positioned in the RCEP market yet, e.g. Cereal, flour, starch, milk preparations and products (19) and Manmade staple fibres (55). If sufficient investment decisions and marketing strategies are applied to these commodities, they will well penetrate the RCEP market and bring trade creation and welfare improvement to Vietnam. Public and private investment should consider the above-mentioned commodities as targets to leapfrog the benefits of RCEP.

Geo-Economic Competition in Latin America: Brazil, Venezuela, and Regional Integration in the 21st Century

2018 ◽  
Vol 33 ◽  
Author(s):  
Guilherme Casarões
Keyword(s):  
Latin American ◽  
Regional Integration ◽  
Free Trade Area ◽  
The United States ◽  
Economic Competition ◽  
Integration Model ◽  
Trade Area ◽  
Complex Processes ◽  
The Us ◽  
New Institutions

The institutional framework of Latin American integration saw a period of intense transformation in the 2000s, with the death of the ambitious project of the Free Trade Area of the Americas (FTAA), spearheaded by the United States, and the birth of two new institutions, the Union of South American Nations (UNASUR) and the Community of Latin American and Caribbean States (CELAC). This article offers a historical reconstruction of regional integration structures in the 2000s, with emphasis on the fault lines between Brazil, Venezuela and the US, and how they have shaped the institutional order across the hemisphere. We argue that the shaping of UNASUR and CELAC, launched respectively in 2007 and 2010, is the outcome of three complex processes: (1) Brazil’s struggle to strengthen Mercosur by acting more decisively as a regional paymaster; (2) Washington’s selective engagement with some key regional players, notably Colombia, and (3) Venezuela’s construction of an alternative integration model through the Bolivarian Alliance (ALBA) and oil diplomacy. If UNASUR corresponded to Brazil’s strategy to neutralize the growing role of Caracas in South America and to break apart the emerging alliance between Venezuela, Argentina, and Bolivia, CELAC was at the same time a means to keep the US away from regional decisions, and to weaken the Caracas-Havana axis that sustained ALBA.

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