Renal handling of phenol red. II. The mechanism of substituted phenolsulphophthalein (PSP) dye transport in rabbit kidney tubules in vitro.

Phenol red (phenolsulfonphthalein, PSP) is thought to be secreted by proximal kidney tubules in all vertebrates. The present study examined PSP transport by the kidney of the salamander, Necturus maculosus. In Necturus kidneys perfused with oxygenated Ringer solution, the PSP/creatinine clearance ratio was unity. Perfusion with 1 mM octanoate converted net p-aminohippurate (PAH) reabsorption to net secretion, but had no effect on PSP. In seven urethan-anesthetized Necturi, the PSP/inulin clearance ratio averaged 0.85 +/- 0.21 (SD), not significantly different from unity. Thin slices from Necturus kidneys incubated in vitro for 2 h failed to accumulate PSP; slice-to-medium (S/M) concentration ratios averaged 0.8 +/- 0.2 (n = 6). With frog kidney slices, (S/M)PSP was 9.6 +/- 1.4 (n = 6). Necturus kidney slices accumulated PAH ((S/M)PAH = 4.1 +/- 0.7) (n = 6), but uptake was not inhibited by 1 mM PSP. We conclude that Necturus kidney tubules transport PAH, but do not transport PSP. These results are consistent with the hypothesis that the organic acid secretory system in most animals involves several carriers.

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Renal handling of phenol red. IV. Tubular localization in rabbit and rat kidney in vivo

AJP Renal Physiology ◽

10.1152/ajprenal.1980.238.3.f159 ◽

1980 ◽

Vol 238 (3) ◽

pp. F159-F165

Author(s):

M. I. Sheikh ◽

J. V. Moller

Keyword(s):

Rat Kidney ◽

Plasma Concentrations ◽

High Plasma ◽

Tubular Secretion ◽

Rabbit Kidney ◽

High Dose ◽

Phenol Red ◽

Renal Handling ◽

Pars Recta

In the rabbit kidney accumulation of phenol red in cortex tissue is directly related to phenol red excretion. In histological preparations of rabbit cortex the major part of phenol red is localized to the pars recta of proximal tubules at low plasma concentrations of dye. The extra uptake of dye by the pars recta is abolished by administration of a high dose of probenecid and also by high plasma dye concentrations, when dye secretion is low relative to tubular reabsorption. Tissue accumulation of phenol red in the rat exhibits features similar to those in the rabbit. However, extra dye uptake in the pars recta is maintained after administration of probenecid, and disappears after intravenous injection of phenol red during ureteral occlusion to impede access of dye from tubule fluid to the luminal membrane. It is concluded that in the rabbit phenol red uptake by the pars recta probably is due to tubular secretion across the peritubular membrane, whereas in the rat extra uptake of dye by this segment is consistent with uptake at the luminal cell membrane.

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A comparative study on the accumulation of probenecid and analogues in rabbit kidney tubules in vitro

Biochemical Pharmacology ◽

10.1016/0006-2952(79)90263-6 ◽

1979 ◽

Vol 28 (1) ◽

pp. 15-22 ◽

Cited By ~ 2

Author(s):

M. Iqbal Sheikh ◽

Marta Stahl ◽

Christian Jacobsen

Keyword(s):

Comparative Study ◽

Rabbit Kidney ◽

Kidney Tubules

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The mechanism of urate transport in rabbit kidney tubules in vitro

Pflügers Archiv - European Journal of Physiology ◽

10.1007/bf00588357 ◽

1971 ◽

Vol 325 (3) ◽

pp. 235-246 ◽

Cited By ~ 8

Author(s):

M. Iqbal Sheikh ◽

Jesper V. M�ller

Keyword(s):

Rabbit Kidney ◽

Kidney Tubules ◽

Urate Transport

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Renal handling of phenol red. I. A comparative study on the accumulation of phenol red andp-aminohippurate in rabbit kidney tubulesin vitro

The Journal of Physiology ◽

10.1113/jphysiol.1972.sp010048 ◽

1972 ◽

Vol 227 (2) ◽

pp. 565-590 ◽

Cited By ~ 21

Author(s):

M. Iqbal Sheikh

Keyword(s):

Comparative Study ◽

Rabbit Kidney ◽

Phenol Red ◽

Renal Handling

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Effects of DOCA, K loading, and cations on K uptake by rabbit kidney cortex

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1962.203.6.1001 ◽

1962 ◽

Vol 203 (6) ◽

pp. 1001-1004 ◽

Cited By ~ 1

Author(s):

W. O. Berndt ◽

D. A. LeSher

Keyword(s):

External Source ◽

Potassium Uptake ◽

Metabolic Inhibitors ◽

Secretory Process ◽

Rabbit Kidney ◽

Kidney Cortex ◽

Fresh Tissue ◽

Renal Handling ◽

Cortex Slices

In previous studies the nature of the substrate requirements, as well as the effects of metabolic inhibitors on potassium uptake by rabbit renal cortex slices, have been examined. In this study a variety of factors have been examined, some of which are known to influence the renal handling of potassium in the intact animal. Pretreatment with deoxycorticosterone acetate enhanced the ability of renal slices to take up potassium from a low external source. This was true even though the fresh-tissue levels of potassium were not markedly altered. Pretreatment with potassium chloride elevated the fresh-tissue potassium level but had no effect on potassium uptake by the slices. Two organic bases which have been reported to interact with the potassium secretory process in the dog were found to have no effect on potassium uptake in vitro. Ammonium chloride was found to depress potassium uptake when present in a concentration equimolar to that of potassium.

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Evaluación in vitro de la microfiltración de Enterococcus faecalis usando cinco técnicas de obturación / In Vitro Evaluation of Enterococcus faecalis Microleakage Using Five Obturation Techniques

Universitas Odontologica ◽

10.11144/javeriana.uo35-74.emef ◽

2016 ◽

Vol 35 (74) ◽

Author(s):

Silvia Elena Aragón Matamoros ◽

Tomás Alberto Guindos Moya ◽

Yanina Meza González ◽

Diana Morales Herrera ◽

María Alejandra Perera Díaz ◽

...

Keyword(s):

Enterococcus Faecalis ◽

Color Change ◽

Three Dimensional ◽

Brain Heart Infusion ◽

Phenol Red ◽

Root Canal Filling ◽

Gutta Percha ◽

Single Cone ◽

Primary Lateral

ABSTRACT. Background: The root canal filling technique named Hybrid-Mixed Condensation, combines the advantages of cold lateral and warm vertical condensation. The ability of avoiding microbial microleakage has not been proven. Purpose: To evaluate the differences of microbial microleakage using Enterococcus faecalis, in canals obturated with five different techniques: lateral, warm vertical, WaveOne® single cone, Guttacore®, and Hybrid-mixed condensation. Methods: 50 single-rooted human premolars extracted for orthodontic reasons were biomechanical prepared with primary file of WaveOne® system. Teeth were divided into 5 groups using different obturation techniques: single cone with WaveOne® Primary, lateral condensation using 2 % gutta-percha cones, Guttacore® 30, warm vertical condensation using down packing in a WaveOne® Primary cone and backfill with alpha gutapercha of Beefill®, and the hybrid mixed condensation modifying the lateral condensation with heat and a backfill using Beefill®. Enterococcus faecalis was inoculated in the coronal third and apices were immersed in brain heart infusion broth with phenol red incubated at 37 °C for 12 weeks. Microfiltration was determined with color change and turbidity of the medium. Specimens were observed by scanning electron microscopy. Results: Only 11 teeth (22 %) were positive for leakage. 46 % with single cone, 30 % with Guttacore®, 20 % with lateral condensation, 10 % with warm vertical condensation and no microleakage was found for Hybrid-Mixed Technique over the period of 12 weeks of study. Conclusion: Hybrid Mixed Technique showed to be the most efficient technique to get three-dimensional seal and prevent microbial contamination of canals in the endodontic therapy.

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