scholarly journals Time course of release of catecholamine and other granular contents from perifused adrenal chromaffin cells of guinea-pig.

1983 ◽  
Vol 341 (1) ◽  
pp. 153-167 ◽  
Author(s):  
S Ito
Keyword(s):  
Guinea Pig ◽  
Chromaffin Cells ◽  
Time Course ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Localization of Muscarinic Receptor and Cation Channel in Guinea-Pig Adrenal Chromaffin Cells

2005 ◽  
Vol 38 (4) ◽  
pp. 273-282 ◽  
Author(s):  
Yutaka Endo ◽  
Keita Harada ◽  
Naoji Fujishiro ◽  
Issei Imanaga ◽  
Koichi Ogawa ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Muscarinic Receptor ◽  
Chromaffin Cells ◽  
Cation Channel ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells

1996 ◽  
Vol 431 (3) ◽  
pp. 402-407 ◽  
Author(s):  
Ken-ichi Otstuguro ◽  
Toshio Ohta ◽  
Shigeo Ito ◽  
Yoshikazu Nakazato
Keyword(s):  
Guinea Pig ◽  
Calcium Current ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Distinct effects of alpha-SNAP, 14-3-3 proteins, and calmodulin on priming and triggering of regulated exocytosis.

1995 ◽  
Vol 130 (5) ◽  
pp. 1063-1070 ◽  
Author(s):  
L H Chamberlain ◽  
D Roth ◽  
A Morgan ◽  
R D Burgoyne
Keyword(s):  
Membrane Fusion ◽  
Chromaffin Cells ◽  
Time Course ◽  
Mechanisms Of Action ◽  
Catecholamine Secretion ◽  
Adrenal Chromaffin Cells ◽  
Regulated Exocytosis ◽  
Distinct Stage ◽  
Adrenal Chromaffin

We have used stage-specific assays for MgATP-dependent priming and for Ca(2+)-activated triggering in the absence of free MgATP to examine the effects of alpha-SNAP, 14-3-3 proteins and calmodulin on regulated exocytosis in permeabilized adrenal chromaffin cells. All three proteins lead to a Ca(2+)-dependent increase in catecholamine secretion. Both alpha-SNAP and 14-3-3 proteins stimulated in a priming but not in a triggering assay. In contrast, calmodulin was stimulatory in triggering but not priming. The effects of alpha-SNAP and 14-3-3 proteins were likely to be due to distinct mechanisms of action since they differed in Ca(2+)-dependency, time course and extent of stimulation and their effects were additive. alpha-SNAP and 14-3-3 proteins did not appear to exert their priming action through changes in synthesis of phosphatidylinositol (4,5) bisphosphate. The data show that these three proteins have distinct stage-specific actions on exocytosis and indicate that alpha-SNAP acts in an early MgATP-requiring stage and not in the late Ca(2+)-triggered steps immediately prior to membrane fusion as previously suggested.

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