Met-enkephalin-Arg6-Gly7-Leu8 in large-cored vesicles of splanchnic nerve terminals innervating guinea pig adrenal chromaffin cells

1985 ◽  
Vol 53 (3) ◽  
pp. 247-252 ◽  
Author(s):  
Shigeru Kobayashi ◽  
Takao Miyabayashi ◽  
Takashi Uchida ◽  
Noboru Yanaihara
Keyword(s):  
Guinea Pig ◽  
Chromaffin Cells ◽  
Splanchnic Nerve ◽  
Nerve Terminals ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Localization of Muscarinic Receptor and Cation Channel in Guinea-Pig Adrenal Chromaffin Cells

2005 ◽  
Vol 38 (4) ◽  
pp. 273-282 ◽  
Author(s):  
Yutaka Endo ◽  
Keita Harada ◽  
Naoji Fujishiro ◽  
Issei Imanaga ◽  
Koichi Ogawa ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Muscarinic Receptor ◽  
Chromaffin Cells ◽  
Cation Channel ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells

1996 ◽  
Vol 431 (3) ◽  
pp. 402-407 ◽  
Author(s):  
Ken-ichi Otstuguro ◽  
Toshio Ohta ◽  
Shigeo Ito ◽  
Yoshikazu Nakazato
Keyword(s):  
Guinea Pig ◽  
Calcium Current ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Suppression of Ca2+ syntillas increases spontaneous exocytosis in mouse adrenal chromaffin cells

2009 ◽  
Vol 134 (4) ◽  
pp. 267-280 ◽  
Author(s):  
Jason J. Lefkowitz ◽  
Kevin E. Fogarty ◽  
Lawrence M. Lifshitz ◽  
Karl D. Bellve ◽  
Richard A. Tuft ◽  
...  
Keyword(s):  
Simple Model ◽  
Chromaffin Cells ◽  
Ryanodine Receptors ◽  
Nerve Terminals ◽  
Adrenal Chromaffin Cells ◽  
Central Concept ◽  
Voltage Gated ◽  
Adrenal Chromaffin ◽  
Synaptic Structures ◽  
Ca2 Channels

A central concept in the physiology of neurosecretion is that a rise in cytosolic [Ca2+] in the vicinity of plasmalemmal Ca2+ channels due to Ca2+ influx elicits exocytosis. Here, we examine the effect on spontaneous exocytosis of a rise in focal cytosolic [Ca2+] in the vicinity of ryanodine receptors (RYRs) due to release from internal stores in the form of Ca2+ syntillas. Ca2+ syntillas are focal cytosolic transients mediated by RYRs, which we first found in hypothalamic magnocellular neuronal terminals. (scintilla, Latin for spark; found in nerve terminals, normally synaptic structures.) We have also observed Ca2+ syntillas in mouse adrenal chromaffin cells. Here, we examine the effect of Ca2+ syntillas on exocytosis in chromaffin cells. In such a study on elicited exocytosis, there are two sources of Ca2+: one due to influx from the cell exterior through voltage-gated Ca2+ channels, and that due to release from intracellular stores. To eliminate complications arising from Ca2+ influx, we have examined spontaneous exocytosis where influx is not activated. We report here that decreasing syntillas leads to an increase in spontaneous exocytosis measured amperometrically. Two independent lines of experimentation each lead to this conclusion. In one case, release from stores was blocked by ryanodine; in another, stores were partially emptied using thapsigargin plus caffeine, after which syntillas were decreased. We conclude that Ca2+ syntillas act to inhibit spontaneous exocytosis, and we propose a simple model to account quantitatively for this action of syntillas.

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