scholarly journals Electrophysiological characterization of native Na+-HCO3-cotransporter current in bovine parotid acinar cells

2005 ◽  
Vol 568 (1) ◽  
pp. 181-197 ◽  
Author(s):  
Souichirou Yamaguchi ◽  
Toru Ishikawa
Keyword(s):  
Acinar Cells ◽  
Parotid Acinar Cells ◽  
Electrophysiological Characterization

Morphological and Functional Characterization of Isolated Human Parotid Acinar Cells

1979 ◽  
Vol 58 (10) ◽  
pp. 2028-2035 ◽  
Author(s):  
John A. Mangos
Keyword(s):  
Functional Characterization ◽  
Acinar Cells ◽  
Mechanical Forces ◽  
Cellular Physiology ◽  
Parotid Acinar Cells

A method for the isolation of human parotid acinar cells using enzymatic dispersion and mild mechanical forces has been developed. The isolated acinar cells are morphologically and functionally intact, thus suitable for studies of the cellular physiology of secretion.

Characterization of Autonomic Receptors in Isolated Human Parotid Acinar Cells

1980 ◽  
Vol 59 (2) ◽  
pp. 168-175 ◽  
Author(s):  
John A. Mangos
Keyword(s):  
Adrenergic Receptors ◽  
Acinar Cells ◽  
Cellular Systems ◽  
Parotid Acinar Cells ◽  
In Vitro Characterization ◽  
Muscarinic Cholinergic ◽  
Autonomic Receptors

Isolated human parotid acinar cells have been used for the in vitro characterization of the muscarinic cholinergic and alpha- and beta-adrenergic receptors of these cells. The agonist-antagonist interactions at the receptor level were studied, and the role of the receptor-activated cellular systems in the process of secretion was characterized.

Dispersed rat parotid acinar cells. III. Characterization of cholinergic receptors

1975 ◽  
Vol 229 (3) ◽  
pp. 566-569 ◽  
Author(s):  
JA Mangos ◽  
NR McSherry ◽  
T Barber
Keyword(s):  
Cellular Response ◽  
Acinar Cells ◽  
Receptor Site ◽  
Cholinergic Receptors ◽  
Cholinergic Agonists ◽  
Cholinergic Agonist ◽  
Parotid Acinar Cells ◽  
Rat Parotid

The in vitro characterization of cholinergic receptors in dispersed rat parotid acinar cells was accomplished through investigations of the net transmembrane fluxes of K in response to exposure of the cells to selected cholinergic agonists and antagonists. Interaction of acetylcholine bromide (ACh) and carbamylcholine (carbachol) with the cholinergic receptors resulted in rapid net efflux of K from the cells. This cellular response was demonstrable in concentrations of carbachol as low as 10(-8) M. With gradual increase in the concentrations of the agonist an increase in the K efflux was observed up to 10(-5) M. At higher concentrations of this cholinergic agonist no further increases in the net K efflux were observed. The response of the cells to cholinergic agonists was inhibited by atropine but not by the adrenergic antagonists phentolamine or propranolol, suggesting cholinergic agonist-antagonist interactions at the receptor site. The dispersed rat parotid acinar cells appear to have functionally intact cholinergic receptors and could be used as valuable experimental tools for the study of receptor physiology and pharmacology as well as of other aspects of secretory function at the cellular level.

scholarly journals Characterization of a phosphorylation event resulting in upregulation of the salivary Na+-K+-2Cl−cotransporter

1999 ◽  
Vol 277 (6) ◽  
pp. C1184-C1193 ◽  
Author(s):  
Kinji Kurihara ◽  
Marilyn L. Moore-Hoon ◽  
Masato Saitoh ◽  
R. James Turner
Keyword(s):  
Binding Sites ◽  
Acinar Cells ◽  
Close Correlation ◽  
Adrenergic Stimulation ◽  
High Affinity ◽  
Parotid Acinar Cells ◽  
Phosphorylation Event ◽  
Rat Parotid ◽  
Stimulation Of

Previous studies from our laboratory have shown a close correlation between increased Na+-K+-2Cl−cotransporter activity and increased cotransporter phosphorylation after β-adrenergic stimulation of rat parotid acinar cells. We demonstrate here that these effects are paralleled by an increase in the number of high-affinity binding sites for the cotransporter inhibitor bumetanide in membranes prepared from stimulated acini. We also show that the sensitivity of cotransporter fluxes to inhibition by bumetanide is the same in both resting and isoproterenol-stimulated cells, consistent with the hypothesis that β-adrenergic stimulation and the accompanying phosphorylation result in the activation of previously quiescent transporters rather than in a change in the properties of already active proteins. In addition, we demonstrate that the increased phosphorylation on the cotransporter resulting from β-adrenergic stimulation is localized to a 30-kDa phosphopeptide obtained by cyanogen bromide digestion. Immunoprecipitation and Western blotting experiments demonstrate that this peptide is derived from the NH2-terminal cytosolic tail of the cotransporter, which surprisingly does not contain the sole protein kinase A consensus site on the molecule.

Dispersed rat parotid acinar cells. II. Characterization of adrenergic receptors

1975 ◽  
Vol 229 (3) ◽  
pp. 560-565 ◽  
Author(s):  
JA Mangos ◽  
NR McSherry ◽  
T Barber ◽  
SN Arvanitakis ◽  
V Wagner
Keyword(s):  
Adrenergic Receptors ◽  
Acinar Cells ◽  
Progressive Increase ◽  
Cellular Level ◽  
Alpha Adrenergic Receptors ◽  
Parotid Acinar Cells ◽  
In Vitro Characterization ◽  
Rat Parotid

The in vitro characterization of adrenergic receptors in isolated rat parotid acinar cells was accomplished through investigations of the transmembrane influxes of K and of the secretion of amylase in response to interactions of the cells with selected agonists and antagonists. Interaction of epinephrine (EPI) at concentrations of 10(-3)-10(-9) M with the alpha-adrenergic receptors resulted in rapid efflux of K from the cells. This effect was inhibited by phentolamine but not by propranolol or atropine. The process of secretion of amylase by these cells involved the activation of the beta-adrenergic receptors by the adrenergic agonists DL-isoproterenol (IPR) and EPI at similar to above concentrations. The interaction of these agonists with the beta receptors was inhibited by propranolol but not by phentolamine or atropine. Dibutyryl clclic AMP stimulated secretion of amylase at concentrations of 10(-8) M. A progressive increase in the secretory response of the cells was observed with increases in the dibutyryl cyclic AMP concentrations up to 10(-5) M. This effect was not inhibited by propranolol. This study demonstrates that dispersed rat parotid acinar cells have functionally intact adrenergic receptors and could be used as experimental tools for the studies of receptor physiology and pharmacology as well as other aspects of secretion at the cellular level.

Characterization of dopamine-induced potassium efflux in rat parotid acinar cells

1988 ◽  
Vol 145 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Staffan Sundström ◽  
Åke Danielsson ◽  
Roger Henriksson ◽  
Per Lindström
Keyword(s):  
Acinar Cells ◽  
Potassium Efflux ◽  
Parotid Acinar Cells ◽  
Rat Parotid
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