Multiphoton imaging of the effects of formaldehyde fixation on tissue autofluorescence

2021 ◽  
Author(s):  
Shu-Jen Chiang ◽  
Po-Hang Tseng ◽  
Jing-Tang Lin ◽  
Chen-Yuan Dong
Keyword(s):  
Multiphoton Imaging

scholarly journals Multiphoton imaging and Raman spectroscopy of the bovine vertebral endplate

The Analyst ◽  
2021 ◽  
Author(s):  
Fay Crawford-Manning ◽  
Martha Z. Vardaki ◽  
Ellen Green ◽  
Judith R. Meakin ◽  
Claudio Vergari ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Vertebral Endplate ◽  
Micro Structure ◽  
Multiphoton Imaging

Multiphoton imaging and Raman spectroscopy reveal chemistry and micro-structure of the bovine vertebral endplate.

scholarly journals Multiphoton Imaging of Ca2+ Instability in Acute Myocardial Slices from a RyR2R2474S Murine Model of Catecholaminergic Polymorphic Ventricular Tachycardia

2021 ◽  
Vol 10 (13) ◽  
pp. 2821
Author(s):  
Giulia Borile ◽  
Tania Zaglia ◽  
Stephan E. Lehnart ◽  
Marco Mongillo
Keyword(s):  
Ventricular Tachycardia ◽  
Single Cells ◽  
Tissue Level ◽  
Catecholaminergic Polymorphic Ventricular Tachycardia ◽  
Polymorphic Ventricular Tachycardia ◽  
Adrenergic Stimulation ◽  
Release Channel ◽  
Multiphoton Imaging ◽  
Delayed Afterdepolarizations ◽  
Multiple Cells

Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) is a familial stress-induced arrhythmia syndrome, mostly caused by mutations in Ryanodine receptor 2 (RyR2), the sarcoplasmic reticulum (SR) Ca2+ release channel in cardiomyocytes. Pathogenetic mutations lead to gain of function in the channel, causing arrhythmias by promoting diastolic spontaneous Ca2+ release (SCR) from the SR and delayed afterdepolarizations. While the study of Ca2+ dynamics in single cells from murine CPVT models has increased our understanding of the disease pathogenesis, questions remain on the mechanisms triggering the lethal arrhythmias at tissue level. Here, we combined subcellular analysis of Ca2+ signals in isolated cardiomyocytes and in acute thick ventricular slices of RyR2R2474S knock-in mice, electrically paced at different rates (1–5 Hz), to identify arrhythmogenic Ca2+ dynamics, from the sub- to the multicellular perspective. In both models, RyR2R2474S cardiomyocytes had increased propensity to develop SCR upon adrenergic stimulation, which manifested, in the slices, with Ca2+ alternans and synchronous Ca2+ release events in neighboring cardiomyocytes. Analysis of Ca2+ dynamics in multiple cells in the tissue suggests that SCRs beget SCRs in contiguous cells, overcoming the protective electrotonic myocardial coupling, and potentially generating arrhythmia triggering foci. We suggest that intercellular interactions may underscore arrhythmic propensity in CPVT hearts with ‘leaky’ RyR2.

Multiphoton imaging with sub-30 fs Yb fiber laser

2012 ◽  
Author(s):  
Ilyas Saytashev ◽  
Bai Nie ◽  
Andy Chong ◽  
Hui Liu ◽  
Sergey Arkhipov ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Multiphoton Imaging

Label-free multiphoton imaging to rapidly classify the early and late-stage tumor necrosis in invasive breast cancer

2021 ◽  
Author(s):  
Yu Yi ◽  
Zhen Li ◽  
Yulan Liu ◽  
Zhijun Li ◽  
Zhonghua Han ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Invasive Breast Cancer ◽  
Late Stage ◽  
Tumor Necrosis ◽  
Label Free ◽  
Multiphoton Imaging

Multiphoton Imaging of Cytotoxic T Lymphocyte-Mediated Antitumor Immune Responses

2009 ◽  
pp. 265-287 ◽  
Author(s):  
Alexandre Boissonnas ◽  
Alix Scholer-Dahire ◽  
Luc Fetler ◽  
Sebastian Amigorena
Keyword(s):  
Immune Responses ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Multiphoton Imaging

scholarly journals Pumpless Microfluidic System for Bone Marrow Niche-on-a-Chip in vitro Modelling and Multiphoton Imaging in Leukemia

2020 ◽  
Vol 118 (3) ◽  
pp. 463a
Author(s):  
Giulia Borile ◽  
Giulia Borella ◽  
Camille Charoy ◽  
Andrea Filippi ◽  
Filippo Romanato ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Microfluidic System ◽  
Bone Marrow Niche ◽  
Multiphoton Imaging ◽  
In Vitro Modelling

P2-164: In vivo multiphoton imaging of neurofibrillary tangles: Relationship to cell death cascades

2008 ◽  
Vol 4 ◽  
pp. T418-T418
Author(s):  
Alix de Calignon ◽  
Tara Spires-Jones ◽  
Bradley T. Hyman
Keyword(s):  
Cell Death ◽  
Neurofibrillary Tangles ◽  
Multiphoton Imaging

scholarly journals Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing

IntraVital ◽  
2012 ◽  
Vol 1 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Xin Liu ◽  
Camilla A. Thorling ◽  
Lu Jin ◽  
Michael S. Roberts
Keyword(s):  
Rat Liver ◽  
Rhodamine 123 ◽  
Multiphoton Imaging
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