Roller-mark is a common problem in offset printing and its solution method is important for printing. A new detecting method of texture analysis was given in this paper. In this study, printing image was acquired with high-speed CCD. Compared the difference between printing image and standard image, a defective image was obtained. Then the reason of roller-marks was given by the texture recognition of defect image. Finally, experiments were taken to prove the feasibility and effectiveness of this new method for the roller-marks diagnosis in the offset printing machine.