The Selective Phosphodiesterase 4 Inhibitor RP 73-401 Reduced Matrix Metalloproteinase 9 Activity and Transforming Growth Factor-β Release During Acute Lung Injury in Mice: The Role of the Balance Between Tumor Necrosis Factor-α and Interleukin-10

ABSTRACT Pemphigus is an autoimmune blistering disease characterized by a loss of cell adhesion result in acantholysis. Genetic factors and immunologic factors such as cytokines particularly IL-1α, IL-10, TNF-α, and TGF-β may counterpart to developing of Pemphigus. The aim of this study was to evaluate. The concentration of IL-1α, IL-10, TNF-α, TGF-α in serum of pemphigus vulgaris (PV) patients and normal individuals. Material and methods In this analytic and descriptive study 25 patients with pemphigus vulgaris (in active phase) and 25 healthy persons were examined. Serum samples of two groups were obtained and the level of IL-1α, IL-10, TNF-α and TGF-β were measured by ELISA technique. The data were analyzed statistically by independent T test (α = 0/05). Results All cytokines tested, showed higher concentration in patient's sera comparing to healthy control individuals. The level of IL-1α (p = 0.004), TNF-α (p = 0.008) and TGF-β (p = 0.009) were statistically different in two experimental groups, There was no significant difference in IL-10 level (p = 0.605). Conclusion Cytokines such as IL-1α, IL-10, TNF-α and TGF-β probably have a role in pathogenesis of PV. Further comprehensive studies are suggested to confirm these findings. How to cite this article Khozeimeh F, Savabi O, Esnaashari M. Evaluation of Interleukin-1α, Interleukin-10, Tumor Necrosis Factor-α and transforming Growth Factor-β in the Serum of Patients with Pemphigus Vulgaris. J Contemp Dent Pract 2014;15(6):746-749.

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Polymorphisms of Tumor Necrosis Factor-α, Transforming Growth Factor-β, and Interleukin-10 in Asthma Associated with Olive Pollen Sensitization

Journal of Immunology Research ◽

10.1155/2014/276345 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Blanca Cárdaba ◽

David Calzada ◽

Selene Baos ◽

Miriam Aguerri ◽

Joaquín Quiralte ◽

...

Keyword(s):

Protective Factor ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Interleukin 10 ◽

Pollen Allergens ◽

Allelic Discrimination ◽

Factor Α ◽

Olive Pollen ◽

Necrosis Factor

Sensitization to specific olive pollen-allergens (Ole e 2 and 10) has been correlated with a clinical pattern of asthma. This study analyzes the association between several polymorphims ofTNFA(G-308A,C-857T, andC-1031T),IL10(C-571AandA-1117G), andTGFB(C-509-T) and these sensitizations. These polymorphisms were genotyped by allelic discrimination, in olive pollen-allergic patients (phenotyped for specific Ole e 2 and 10 sensitizations) and healthy controls. Levels of serum-soluble cytokines were correlated with specific genotypes and clinical phenotypes. The results showed that heterozygousTGFB C-509Tgenotype, besides having the lowest sera TGF- levels, was significantly increased in olive pollen-allergic patients compared with controls. According specific sensitizations,CCgenotype ofIL10 C-571Acould be a protective factor for Ole e 2 sensitization and mainly for asthmatic Ole e 2 sensitized patients compared with asthmatic non-Ole e 2 sensitized patients (OR: 0.26,P=0.008). In contrast, heterozygousCAgenotype was increased in Ole e 2 asthmatic subjects compared to asthmatic non-Ole e 2 sensitized patients. Lastly, heterozygousTNFA G-308Agenotype was associated with Ole e 10 sensitization (OR: 2.5,P=0.04). In conclusion, these results suggest a role of TGF-β1 in olive-pollen sensitization and TNF-αand IL-10 genotypes in the asthma induced by specific olive-pollen allergens.

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