scholarly journals SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress

2022 ◽  
Vol 8 (1) ◽  
Author(s):  
Katharina M. Scherer ◽  
Luca Mascheroni ◽  
George W. Carnell ◽  
Lucia C. S. Wunderlich ◽  
Stanislaw Makarchuk ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Viral Assembly

scholarly journals The SARS-CoV-2 nucleocapsid protein associates with the replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress

2021 ◽  
Author(s):  
Katharina M Scherer ◽  
Luca Mascheroni ◽  
George W Carnell ◽  
Lucia C S Wunderlich ◽  
Stanislaw Makarchuk ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Rna Replication ◽  
Viral Proteins ◽  
Viral Assembly ◽  
Sequence Of Events ◽  
Spatiotemporal Regulation ◽  
Transport Vesicles ◽  
Replication Foci ◽  
Assembly Compartment

Despite being the target of extensive research efforts due to the COVID-19 pandemic, relatively little is known about the dynamics of SARS-CoV-2 replication within cells. We investigate and characterise the tightly orchestrated sequence of events during different stages of the infection cycle by visualising the spatiotemporal dynamics of the four structural proteins of SARS-CoV-2 at high resolution. The nucleoprotein is expressed first and accumulates around folded ER membranes in convoluted layers that connect to viral RNA replication foci. We find that of the three transmembrane proteins, the membrane protein appears at the Golgi apparatus/ERGIC before the spike and envelope proteins. Relocation of the lysosome marker LAMP1 towards the assembly compartment and its detection in transport vesicles of viral proteins confirm an important role of lysosomes in SARS-CoV-2 egress. These data provide new insights into the spatiotemporal regulation of SARS-CoV-2 assembly, and refine current understanding of SARS-CoV-2 replication.

Structure-function of the nucleocapsid protein of PRRSV North American genotype

2000 ◽  
Vol 31 (1) ◽  
pp. 24-25 ◽  
Author(s):  
D. Yoo ◽  
S. Wootton
Keyword(s):  
Structure Function ◽  
North American ◽  
Nucleocapsid Protein ◽  
North American Genotype

Antibody Screening Results for Anti-Nucleocapsid Antibodies Towards the Development of a SARS-CoV-2 Nucleocapsid Protein Antigen Detecting Lateral Flow Assay

2021 ◽  
Author(s):  
David Cate ◽  
Helen Hsieh ◽  
Veronika Glukhova ◽  
Joshua D Bishop ◽  
H Gleda Hermansky ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Point Of Care ◽  
Lateral Flow ◽  
Screening Methods ◽  
Antibody Screening ◽  
Liquid Handling ◽  
Large Numbers ◽  
Accurate Performance ◽  
Further Development ◽  
Assay Conditions

<p></p><p>The global COVID-19 pandemic has created an urgent demand for large numbers of inexpensive, accurate, rapid, point-of-care diagnostic tests. Analyte-based assays are suitably inexpensive and can be rapidly mass-produced, but for sufficiently accurate performance they require highly optimized antibodies and assay conditions. We used an automated liquid handling system, customized to handle arrays of lateral flow immunoassay (LFA) tests in a high-throughput screen, to identify anti-nucleocapsid antibodies that will perform optimally in an LFA. We tested 1021 anti-nucleocapsid antibody pairs as LFA capture and detection reagents with the goal of highlighting pairs that have the greatest affinity for unique epitopes of the nucleocapsid protein of SARS-CoV-2 within the LFA format. In contrast to traditional antibody screening methods (e.g., ELISA, bio-layer interferometry), the method described here integrates real-time reaction kinetics with transport in, and immobilization directly onto, nitrocellulose. We have identified several candidate antibody pairs that are suitable for further development of an LFA for SARS-CoV-2.</p><p></p>

Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein.

1983 ◽  
Vol 45 (2) ◽  
pp. 773-781 ◽  
Author(s):  
J Sprague ◽  
J H Condra ◽  
H Arnheiter ◽  
R A Lazzarini
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Nucleocapsid Protein ◽  
Recombinant Dna ◽  
Gene Coding ◽  
Vesicular Stomatitis

Measles virus nucleocapsid protein protects rats from encephalitis.

1991 ◽  
Vol 65 (4) ◽  
pp. 1695-1700 ◽  
Author(s):  
B Bankamp ◽  
U G Brinckmann ◽  
A Reich ◽  
S Niewiesk ◽  
V ter Meulen ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Measles Virus

scholarly journals The 1H, 15N, and 13C resonance assignments of the N-terminal domain of the nucleocapsid protein from the Middle East respiratory syndrome coronavirus

2021 ◽  
Author(s):  
Talita Stelling de Araujo ◽  
Glauce Moreno Barbosa ◽  
Karoline Sanches ◽  
Jéssica M. Azevedo ◽  
Katia Maria dos Santos Cabral ◽  
...  
Keyword(s):  
Middle East ◽  
Nucleocapsid Protein ◽  
Resonance Assignments ◽  
Middle East Respiratory Syndrome ◽  
Terminal Domain
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