PLANT TISSUE CULTURE AND BIOTECHNOLOGY MEETING: Getting Down to Bare Wood and Overcoming a Barrier

Science ◽  
2002 ◽  
Vol 297 (5587) ◽  
pp. 1637-1638 ◽  
Author(s):  
A. S. Moffat
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue

Development of Plant Tissue Culture Profile of Dioscorea deltoidea

Planta Medica ◽  
2013 ◽  
Vol 79 (05) ◽  
Author(s):  
M Mujeeb ◽  
M Amir ◽  
AS Nadeem ◽  
M Aqil ◽  
AK Najmi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Dioscorea Deltoidea

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

Plant Tissue Culture

1981 ◽  
Vol 43 (9) ◽  
pp. 508-512
Author(s):  
Patricia Pietropaolo
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue

Plant tissue culture manual

1992 ◽  
Vol 31 (9) ◽  
pp. 3301-3302
Author(s):  
Ichard J. Robins
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue

scholarly journals Analysis of bioactive pigments in coloured callus of Azadirachta indica for possible use as functional natural colourants

2019 ◽  
Vol 48 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Sharmilla Ashokhan ◽  
Sujatha Ramasamy ◽  
Saiful Anuar Karsani ◽  
Rashidi Othman ◽  
Jamilah Syafawati Yaacob
Keyword(s):  
Tissue Culture ◽  
Azadirachta Indica ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Content Type ◽  
Bioactive Properties ◽  
Green Callus

Purpose The purpose of this study is to evaluate the content of bioactive pigments in coloured callus of Azadirachta indica and to understand the correlation between the callus colours with their bioactive constituents, antioxidant properties and cytotoxicity. These assessments will yield valuable insight into the use of in vitro-derived pigments for possible use as functional natural colourants. Design/methodology/approach In this study, the authors have successfully developed a protocol to produce leaf-derived callus of various colours with enhanced content of bioactive pigments in A. indica through plant tissue culture. Comparative analysis of the pigments content (chlorophyll, carotenoid, phenolics and anthocyanins) in the coloured callus was conducted, followed by evaluation of its bioactive properties. The antioxidant properties against 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, ferric reducing antioxidant power and cytotox activities of the coloured callus extracts were also reported. Findings Callus of various colours were successfully produced in A. indica through plant tissue culture, and their valuable pigment content and bioactivity were evaluated. The green callus contained the highest amount of anthocyanin, followed by brown and cream callus. The total anthocyanin contents in both the green and brown callus was more than two-fold higher than that in cream callus. Contrasting observation was obtained for total phenolic content (TPC), where the TPC of cream callus was significantly higher than that in brown callus. Nevertheless, the green callus also exhibited the highest TPC. Green callus also contained the highest amount of total chlorophyll and carotenoid, as well as exhibited the highest antioxidant potential, and was found to be the only extract with active cytotox activity against SKOV-3 cells. Correlation analysis revealed that the excellent bioactivity exhibited by the coloured extracts was strongly correlated with the bioactive pigments present in the callus. Research limitations/implications The major bioactive compounds identified in the methanolic extracts of A. indica coloured callus are anthocyanins, phenolics, chlorophylls and carotenoids. Future research work should include improvements in the extraction and identification methods, which may lead to detection and determination of other compounds that could attribute to its bioactivity, to complement the findings of the current study. Practical implications This analysis provides valuable information on the application of plant tissue culture as an alternative source for sustainable production of major pigments with medicinal benefits in A. indica for possible use as functional natural colourants. Originality/value A comparative study on bioactive pigment production in coloured callus from A. indica leaves and its antioxidant potential and cytotoxicity is original. To the best of the authors’ knowledge, this is the first report detailing a comparative evaluation on the production of coloured callus in A. indica and its relative biochemical composition and bioactive properties.

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