In vitro model for stretch-induced hypertrophy of skeletal muscle

Science ◽  
1979 ◽  
Vol 203 (4377) ◽  
pp. 265-268 ◽  
Author(s):  
H Vandenburgh ◽  
S Kaufman
Keyword(s):  
Skeletal Muscle ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0232081 ◽  
Author(s):  
Anna Urciuolo ◽  
Elena Serena ◽  
Rusha Ghua ◽  
Susi Zatti ◽  
Monica Giomo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Single Fiber ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals Skeletal muscle-on-a-chip: an in vitro model to evaluate tissue formation and injury

Lab on a Chip ◽  
2017 ◽  
Vol 17 (20) ◽  
pp. 3447-3461 ◽  
Author(s):  
Gaurav Agrawal ◽  
Aereas Aung ◽  
Shyni Varghese
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Tissue Formation ◽  
Microfluidic Platform ◽  
Muscle Tissues ◽  
Vitro Model

We introduce a microfluidic platform in which we culture three-dimensional skeletal muscle tissues, while evaluating tissue formation and toxin-induced muscle injury.

scholarly journals Electrical Pulse Stimulation of Cultured Human Skeletal Muscle Cells as an In Vitro Model of Exercise

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33203 ◽  
Author(s):  
Nataša Nikolić ◽  
Siril Skaret Bakke ◽  
Eili Tranheim Kase ◽  
Ida Rudberg ◽  
Ingeborg Flo Halle ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Muscle Cells ◽  
Electrical Pulse Stimulation ◽  
Human Skeletal Muscle Cells ◽  
Vitro Model ◽  
Pulse Stimulation ◽  
Stimulation Of

3A1-I01 Development of in vitro model with photoresponsive skeletal muscle(Bio Manipulation)

2014 ◽  
Vol 2014 (0) ◽  
pp. _3A1-I01_1-_3A1-I01_3
Author(s):  
Toshifumi ASANO ◽  
Toru ISHIZUKA ◽  
Hiromu YAWO ◽  
Keisuke MORISHIMA
Keyword(s):  
Skeletal Muscle ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Murine Muscle Engineered from Dermal Precursors: An In Vitro Model for Skeletal Muscle Generation, Degeneration, and Fatty Infiltration

2014 ◽  
Vol 20 (1) ◽  
pp. 28-41 ◽  
Author(s):  
Patricia García-Parra ◽  
Neia Naldaiz-Gastesi ◽  
Marcos Maroto ◽  
Juan Fernando Padín ◽  
María Goicoechea ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
In Vitro Model ◽  
Fatty Infiltration ◽  
Vitro Model

Development of an in vitro model for study of the efficacy of ischemic preconditioning in human skeletal muscle against ischemia-reperfusion injury

2006 ◽  
Vol 101 (5) ◽  
pp. 1335-1342 ◽  
Author(s):  
Glyka Martou ◽  
Catherine A. O’Blenes ◽  
Ning Huang ◽  
Sandra E. McAllister ◽  
Peter C. Neligan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protective Effect ◽  
Ischemic Preconditioning ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Ischemia Reperfusion ◽  
Mtt Reduction ◽  
Vitro Model ◽  
Hypoxia Reoxygenation

Ischemia-reperfusion (I/R) injury causes skeletal muscle infarction and ischemic preconditioning (IPC) augments ischemic tolerance in animal models. To date, this has not been demonstrated in human skeletal muscle. This study aimed to develop an in vitro model to investigate the efficacy of simulated IPC in human skeletal muscle. Human skeletal muscle strips were equilibrated in oxygenated Krebs-Henseleit-HEPES buffer (37°C). Aerobic and reperfusion phases were simulated by normoxic incubation and reoxygenation, respectively. Ischemia was simulated by hypoxic incubation. Energy store, cell viability, and cellular injury were assessed using ATP, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT), and lactate dehydrogenase (LDH) assays, respectively. Morphological integrity was assessed using electron microscopy. Studies were designed to test stability of the preparation ( n = 5–11) under normoxic incubation over 24 h; the effect of 1, 2, 3, 4, or 6 h hypoxia followed by 2 h of reoxygenation; and the protective effect of hypoxic preconditioning (HPC; 5 min of hypoxia/5 min of reoxygenation) before 3 h of hypoxia/2 h of reoxygenation. Over 24 h of normoxic incubation, muscle strips remained physiologically intact as assessed by MTT, ATP, and LDH assays. After 3 h of hypoxia/2 h of reoxygenation, MTT reduction levels declined to 50.1 ± 5.5% ( P < 0.05). MTT reduction levels in HPC (82.3 ± 10.8%) and normoxic control (81.3 ± 10.2%) groups were similar and higher ( P < 0.05) than the 3 h of hypoxia/2 h of reoxygenation group (45.2 ± 5.8%). Ultrastructural morphology was preserved in normoxic and HPC groups but not in the hypoxia/reoxygenation group. This is the first study to characterize a stable in vitro model of human skeletal muscle and to demonstrate a protective effect of HPC in human skeletal muscle against hypoxia/reoxygenation-induced injury.

Normalizing Glutamine Concentration Causes Mitochondrial Uncoupling in an In Vitro Model of Human Skeletal Muscle

2013 ◽  
Vol 39 (2) ◽  
pp. 180-189 ◽  
Author(s):  
Adela Krajcova ◽  
Jakub Ziak ◽  
Katerina Jiroutkova ◽  
Jana Patkova ◽  
Moustafa Elkalaf ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Glutamine Concentration ◽  
Mitochondrial Uncoupling ◽  
Vitro Model
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