scholarly journals An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser

Science ◽  
2019 ◽  
Vol 366 (6463) ◽  
pp. 334-338 ◽  
Author(s):  
Michihiro Suga ◽  
Fusamichi Akita ◽  
Keitaro Yamashita ◽  
Yoshiki Nakajima ◽  
Go Ueno ◽  
...  
Keyword(s):  
Free Electron ◽  
Water Oxidation ◽  
Free Electron Laser ◽  
Structural Changes ◽  
Water Channel ◽  
X Ray ◽  
Cooperative Action ◽  
Oxygen Oxygen ◽  
Photosynthetic Water Oxidation ◽  
S States

Photosynthetic water oxidation is catalyzed by the Mn4CaO5 cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S0 to S4). To reveal the mechanism of water oxidation, we analyzed structures of PSII in the S1, S2, and S3 states by x-ray free-electron laser serial crystallography. No insertion of water was found in S2, but flipping of D1 Glu189 upon transition to S3 leads to the opening of a water channel and provides a space for incorporation of an additional oxygen ligand, resulting in an open cubane Mn4CaO6 cluster with an oxyl/oxo bridge. Structural changes of PSII between the different S states reveal cooperative action of substrate water access, proton release, and dioxygen formation in photosynthetic water oxidation.

scholarly journals The Mn 4 Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130324 ◽  
Author(s):  
Rosalie Tran ◽  
Jan Kern ◽  
Johan Hattne ◽  
Sergey Koroidov ◽  
Julia Hellmich ◽  
...  
Keyword(s):  
Free Electron ◽  
Water Oxidation ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Oxidation Catalyst ◽  
Cryogenic Temperatures ◽  
New Approach ◽  
X Ray ◽  
The Past ◽  
Photosynthetic Water Oxidation

The structure of photosystem II and the catalytic intermediate states of the Mn 4 CaO 5 cluster involved in water oxidation have been studied intensively over the past several years. An understanding of the sequential chemistry of light absorption and the mechanism of water oxidation, however, requires a new approach beyond the conventional steady-state crystallography and X-ray spectroscopy at cryogenic temperatures. In this report, we present the preliminary progress using an X-ray free-electron laser to determine simultaneously the light-induced protein dynamics via crystallography and the local chemistry that occurs at the catalytic centre using X-ray spectroscopy under functional conditions at room temperature.

X-Ray Free Electron Laser Facility for Next Generation of Scientific Exploration and Discovery

2011 ◽  
Vol 131 (2) ◽  
pp. 68-71
Author(s):  
Etsuo FUJIWARA ◽  
Eiichi ANAYAMA ◽  
Yuichiro KATSUTA ◽  
Toshiki IZUTANI ◽  
Daichi OKUHARA ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Next Generation ◽  
X Ray ◽  
Scientific Exploration ◽  
Laser Facility

X-ray Free Electron Laser SACLA

2014 ◽  
Vol 134 (12) ◽  
pp. 836-839
Author(s):  
Junichi INOUE ◽  
Yuji TANAKA ◽  
Yuki MATSUMOTO ◽  
Kensuke KANDA
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
X Ray

scholarly journals Production of superconducting 1.3-GHz cavities for the European X-ray Free Electron Laser

2016 ◽  
Vol 19 (9) ◽  
Author(s):  
W. Singer ◽  
A. Brinkmann ◽  
R. Brinkmann ◽  
J. Iversen ◽  
A. Matheisen ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
X Ray

scholarly journals Single- and two-color attosecond hard x-ray free-electron laser pulses with nonlinear compression

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Alexander Malyzhenkov ◽  
Yunieski P. Arbelo ◽  
Paolo Craievich ◽  
Philipp Dijkstal ◽  
Eugenio Ferrari ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Laser Pulses ◽  
X Ray ◽  
Nonlinear Compression
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