scholarly journals The Mn 4 Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130324 ◽  
Author(s):  
Rosalie Tran ◽  
Jan Kern ◽  
Johan Hattne ◽  
Sergey Koroidov ◽  
Julia Hellmich ◽  
...  
Keyword(s):  
Free Electron ◽  
Water Oxidation ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Oxidation Catalyst ◽  
Cryogenic Temperatures ◽  
New Approach ◽  
X Ray ◽  
The Past ◽  
Photosynthetic Water Oxidation

The structure of photosystem II and the catalytic intermediate states of the Mn 4 CaO 5 cluster involved in water oxidation have been studied intensively over the past several years. An understanding of the sequential chemistry of light absorption and the mechanism of water oxidation, however, requires a new approach beyond the conventional steady-state crystallography and X-ray spectroscopy at cryogenic temperatures. In this report, we present the preliminary progress using an X-ray free-electron laser to determine simultaneously the light-induced protein dynamics via crystallography and the local chemistry that occurs at the catalytic centre using X-ray spectroscopy under functional conditions at room temperature.

scholarly journals An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser

Science ◽  
2019 ◽  
Vol 366 (6463) ◽  
pp. 334-338 ◽  
Author(s):  
Michihiro Suga ◽  
Fusamichi Akita ◽  
Keitaro Yamashita ◽  
Yoshiki Nakajima ◽  
Go Ueno ◽  
...  
Keyword(s):  
Free Electron ◽  
Water Oxidation ◽  
Free Electron Laser ◽  
Structural Changes ◽  
Water Channel ◽  
X Ray ◽  
Cooperative Action ◽  
Oxygen Oxygen ◽  
Photosynthetic Water Oxidation ◽  
S States

Photosynthetic water oxidation is catalyzed by the Mn4CaO5 cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S0 to S4). To reveal the mechanism of water oxidation, we analyzed structures of PSII in the S1, S2, and S3 states by x-ray free-electron laser serial crystallography. No insertion of water was found in S2, but flipping of D1 Glu189 upon transition to S3 leads to the opening of a water channel and provides a space for incorporation of an additional oxygen ligand, resulting in an open cubane Mn4CaO6 cluster with an oxyl/oxo bridge. Structural changes of PSII between the different S states reveal cooperative action of substrate water access, proton release, and dioxygen formation in photosynthetic water oxidation.

scholarly journals Liquid sample delivery techniques for serial femtosecond crystallography

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130337 ◽  
Author(s):  
Uwe Weierstall
Keyword(s):  
Flow Rate ◽  
Free Electron ◽  
Repetition Rate ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Liquid Flow Rate ◽  
Free Electron Lasers ◽  
Liquid Sample ◽  
X Ray ◽  
Serial Femtosecond Crystallography

X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro- and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at warm linac-based free-electron lasers to 1 MHz at superconducting linacs. Liquid injectors are therefore an essential part of a serial femtosecond crystallography experiment at an X-ray free-electron laser. Here, we compare different techniques of injecting microcrystals in solution into the pulsed X-ray beam in vacuum. Sample waste due to mismatch of the liquid flow rate to the X-ray repetition rate can be addressed through various techniques.

Determination of x-ray free electron laser power using a room-temperature calorimeter

Metrologia ◽  
2016 ◽  
Vol 53 (1) ◽  
pp. 98-102 ◽  
Author(s):  
T Tanaka ◽  
M Kato ◽  
N Saito ◽  
K Tono ◽  
M Yabashi ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Laser Power ◽  
Room Temperature ◽  
X Ray

scholarly journals Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser

2019 ◽  
Vol 75 (7) ◽  
pp. 496-500
Author(s):  
Christopher Jones ◽  
Brandon Tran ◽  
Chelsie Conrad ◽  
Jason Stagno ◽  
Robert Trachman ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Small Molecules ◽  
Free Electron ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Temperature Structure ◽  
Data Set ◽  
Time Resolved ◽  
Regulate Gene Expression ◽  
X Ray

Riboswitches are conformationally dynamic RNAs that regulate gene expression by binding specific small molecules. ZTP riboswitches bind the purine-biosynthetic intermediate 5-aminoimidazole-4-carboxamide riboside 5′-monophosphate (ZMP) and its triphosphorylated form (ZTP). Ligand binding to this riboswitch ultimately upregulates genes involved in folate and purine metabolism. Using an X-ray free-electron laser (XFEL), the room-temperature structure of the Fusobacterium ulcerans ZTP riboswitch bound to ZMP has now been determined at 4.1 Å resolution. This model, which was refined against a data set from ∼750 diffraction images (each from a single crystal), was found to be consistent with that previously obtained from data collected at 100 K using conventional synchrotron X-radiation. These experiments demonstrate the feasibility of time-resolved XFEL experiments to understand how the ZTP riboswitch accommodates cognate ligand binding.

scholarly journals X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

2017 ◽  
Vol 18 (9) ◽  
pp. 1918 ◽  
Author(s):  
Christopher Hutchison ◽  
Violeta Cordon-Preciado ◽  
Rhodri Morgan ◽  
Takanori Nakane ◽  
Josie Ferreira ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Free Electron ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Fluorescent Protein ◽  
X Ray

X-Ray Free Electron Laser Facility for Next Generation of Scientific Exploration and Discovery

2011 ◽  
Vol 131 (2) ◽  
pp. 68-71
Author(s):  
Etsuo FUJIWARA ◽  
Eiichi ANAYAMA ◽  
Yuichiro KATSUTA ◽  
Toshiki IZUTANI ◽  
Daichi OKUHARA ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Next Generation ◽  
X Ray ◽  
Scientific Exploration ◽  
Laser Facility

X-ray Free Electron Laser SACLA

2014 ◽  
Vol 134 (12) ◽  
pp. 836-839
Author(s):  
Junichi INOUE ◽  
Yuji TANAKA ◽  
Yuki MATSUMOTO ◽  
Kensuke KANDA
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
X Ray
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