scholarly journals Multiple Routes for Glutamate Receptor Trafficking: Surface Diffusion and Membrane Traffic Cooperate to Bring Receptors to Synapses

2006 ◽  
Vol 2006 (327) ◽  
pp. pe13-pe13 ◽  
Author(s):  
L. Cognet ◽  
L. Groc ◽  
B. Lounis ◽  
D. Choquet
Keyword(s):  
Surface Diffusion ◽  
Glutamate Receptor ◽  
Membrane Traffic ◽  
Receptor Trafficking ◽  
Glutamate Receptor Trafficking ◽  
Multiple Routes

scholarly journals Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kento Ojima ◽  
Kazuki Shiraiwa ◽  
Kyohei Soga ◽  
Tomohiro Doura ◽  
Mikiko Takato ◽  
...  
Keyword(s):  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Protein Labeling ◽  
Selective Labeling ◽  
Receptor Localization ◽  
Glutamate Receptor Trafficking ◽  
Versatile Tool ◽  
The Central Nervous System ◽  
Long Lifetime

AbstractThe regulation of glutamate receptor localization is critical for development and synaptic plasticity in the central nervous system. Conventional biochemical and molecular biological approaches have been widely used to analyze glutamate receptor trafficking, especially for α-amino-3-hydroxy-5-methyl-4-isoxazole-propionate-type glutamate receptors (AMPARs). However, conflicting findings have been reported because of a lack of useful tools for analyzing endogenous AMPARs. Here, we develop a method for the rapid and selective labeling of AMPARs with chemical probes, by combining affinity-based protein labeling and bioorthogonal click chemistry under physiological temperature in culture medium. This method allows us to quantify AMPAR distribution and trafficking, which reveals some unique features of AMPARs, such as a long lifetime and a rapid recycling in neurons. This method is also successfully expanded to selectively label N-methyl-D-aspartate-type glutamate receptors. Thus, bioorthogonal two-step labeling may be a versatile tool for investigating the physiological and pathophysiological roles of glutamate receptors in neurons.

scholarly journals Role for A Kinase-anchoring Proteins (AKAPS) in Glutamate Receptor Trafficking and Long Term Synaptic Depression

2005 ◽  
Vol 280 (17) ◽  
pp. 16962-16968 ◽  
Author(s):  
Eric M. Snyder ◽  
Marcie Colledge ◽  
Robert A. Crozier ◽  
Wendy S. Chen ◽  
John D. Scott ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Synaptic Depression ◽  
Receptor Trafficking ◽  
Anchoring Proteins ◽  
Glutamate Receptor Trafficking

scholarly journals Glutamate Receptor Trafficking and Protein Synthesis Mediate the Facilitation of LTP by Secreted Amyloid Precursor Protein-Alpha

2019 ◽  
Vol 39 (17) ◽  
pp. 3188-3203 ◽  
Author(s):  
Bruce G. Mockett ◽  
Diane Guévremont ◽  
Megan K. Elder ◽  
Karen D. Parfitt ◽  
Katie Peppercorn ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amyloid Precursor Protein ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Precursor Protein ◽  
Glutamate Receptor Trafficking

scholarly journals Crystal structures of autoinhibitory PDZ domain of Tamalin: implications for metabotropic glutamate receptor trafficking regulation

2007 ◽  
Vol 26 (8) ◽  
pp. 2192-2205 ◽  
Author(s):  
Takuma Sugi ◽  
Takuji Oyama ◽  
Takanori Muto ◽  
Shigetada Nakanishi ◽  
Kosuke Morikawa ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Pdz Domain ◽  
Receptor Trafficking ◽  
Metabotropic Glutamate ◽  
Glutamate Receptor Trafficking

Glutamate Receptor Trafficking in Synaptic Plasticity

2002 ◽  
Vol 2002 (156) ◽  
pp. re14-re14 ◽  
Author(s):  
A. Contractor ◽  
S. F. Heinemann
Keyword(s):  
Synaptic Plasticity ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Glutamate Receptor Trafficking

scholarly journals Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking

2020 ◽  
Author(s):  
Kento Ojima ◽  
Kazuki Shiraiwa ◽  
Tomohiro Doura ◽  
Mikiko Takato ◽  
Kazuhiro Komatsu ◽  
...  
Keyword(s):  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Protein Labeling ◽  
Selective Labeling ◽  
Receptor Localization ◽  
Glutamate Receptor Trafficking ◽  
Versatile Tool ◽  
The Central Nervous System ◽  
Long Lifetime

ABSTRACTThe regulation of glutamate receptor localization is critical for development and synaptic plasticity in the central nervous system. Conventional biochemical and molecular biological approaches have been widely used to analyze glutamate receptor trafficking, especially for AMPA-type glutamate receptors (AMPARs). However, conflicting findings have been reported because of a lack of useful tools for analyzing endogenous AMPARs. Here, we develop a new method for the rapid and selective labeling of chemical probes to AMPARs by combining affinity-based protein labeling and bioorthogonal click chemistry under physiological conditions. This method allowed us to quantify AMPAR distribution and trafficking, which revealed some unique features of AMPARs, such as a long lifetime and a rapid recycling in neurons. This method was also successfully expanded to selectively label NMDA-type glutamate receptors. Thus, bioorthogonal two-step labeling may be a versatile tool for investigating the physiological and pathophysiological roles of glutamate receptors in neurons.

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