scholarly journals The Macrolide Resistance Genes erm(B) and mef(E) Are Carried by Tn2010 in Dual-Gene Streptococcus pneumoniae Isolates Belonging to Clonal Complex CC271

2007 ◽  
Vol 51 (11) ◽  
pp. 4184-4186 ◽  
Author(s):  
Maria Del Grosso ◽  
John G. E. Northwood ◽  
David J. Farrell ◽  
Annalisa Pantosti
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Clonal Complex ◽  
Macrolide Resistance ◽  
Genetic Element ◽  
Genetic Elements

ABSTRACT The genetic elements carrying macrolide resistance genes in Streptococcus pneumoniae isolates belonging to CC271 were investigated. The international clone Taiwan19F-14 was found to carry Tn2009, a Tn916-like transposon containing tet(M) and mef(E). The dual erm(B) mef(E) isolates carried Tn2010, which is similar to Tn2009 with the addition of a putative new transposon, the erm(B) genetic element.

scholarly journals Mechanisms of Macrolide Resistance among Streptococcus pneumoniae Isolates from Russia

2008 ◽  
Vol 52 (6) ◽  
pp. 2260-2262 ◽  
Author(s):  
Ralf R. Reinert ◽  
Olga Y. Filimonova ◽  
Adnan Al-Lahham ◽  
Svetlana A. Grudinina ◽  
Elena N. Ilina ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Clonal Complex ◽  
Resistance Mechanisms ◽  
Macrolide Resistance ◽  
Central Russia

ABSTRACT Among 76 macrolide-nonsusceptible Streptococcus pneumoniae isolates collected between 2003 and 2005 from Central Russia, the resistance mechanisms detected in the isolates included erm(B) alone (50%), mef alone [mef(E), mef(I), or a different mef subclass; 19.7%], or both erm(B) and mef(E) (30.3%). Isolates with dual resistance genes [erm(B) and mef(E)] belonged to clonal complex CC81 or CC271.

scholarly journals Genetic Resistance Elements CarryingmefSubclasses Other thanmef(A) in Streptococcus pyogenes

2011 ◽  
Vol 55 (7) ◽  
pp. 3226-3230 ◽  
Author(s):  
Maria Del Grosso ◽  
Romina Camilli ◽  
Giada Barbabella ◽  
John Blackman Northwood ◽  
David J. Farrell ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Streptococcus Pyogenes ◽  
Resistance Genes ◽  
Genetic Resistance ◽  
The Novel ◽  
Genetic Element ◽  
Erythromycin Resistance ◽  
Content Type ◽  
Genetic Elements

ABSTRACTInStreptococcus pyogenes, efflux-mediated erythromycin resistance is associated with themefgene, represented mostly bymef(A), although a small portion of strains carry differentmefsubclasses. We characterized the composite genetic elements, includingmefsubclasses other thanmef(A), associated with other resistance genes inS. pyogenesisolates. Determination of the genetic elements was performed by PCR mapping. The strains carrying mosaicmef(A/E), in which the 5′ region was identical tomef(A) and the 3′ region was identical tomef(E), also carriedtet(O). The two genes were found enclosed in an element similar toS. pyogenesprophage Φm46.1, designated the Φm46.1-like element. InS. pyogenesstrains carryingmef(E) andtet(M),mef(E) was included in a typical mega element, and in some strains, it was physically associated withtet(M) in the composite element Tn2009.S. pyogenesstrains carryingmef(I) also carriedcatQ; the two genes were linked in a fragment representing a portion of the 5216IQ complex ofStreptococcus pneumoniae, designated the defective IQ element. In the only isolate carrying a novelmefgene, this was associated withcatQandtet(M) in a genetic element similar to the 5216IQ complex ofS. pneumoniae(5216IQ-like complex), suggesting that the novelmefis in fact a variant ofmef(I). This study demonstrates that the composite elements containingmefare shared betweenS. pyogenesandS. pneumoniaeand suggests that it is important to distinguish themefsubclass on the basis of the genetic element containing it.

scholarly journals Composite mobile genetic elements disseminating macrolide resistance in Streptococcus pneumoniae

2015 ◽  
Vol 6 ◽  
Author(s):  
Scott T. Chancey ◽  
Sonia Agrawal ◽  
Max R. Schroeder ◽  
Monica M. Farley ◽  
Hervé Tettelin ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Mobile Genetic Elements ◽  
Macrolide Resistance ◽  
Genetic Elements

scholarly journals Emerging non-PCV13 serotypes of noninvasive Streptococcus pneumoniae with macrolide resistance genes in northern Japan

2016 ◽  
Vol 9 ◽  
pp. 66-72 ◽  
Author(s):  
M. Kawaguchiya ◽  
N. Urushibara ◽  
M.S. Aung ◽  
S. Morimoto ◽  
M. Ito ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Macrolide Resistance ◽  
Northern Japan

scholarly journals Emergence of mosaic plasmids harboring Tn1546-ermBelement inStaphylococcus aureusisolates

2018 ◽  
Author(s):  
Tsai-Wen Wan ◽  
Yu-Tzu Lin ◽  
Wei-Chun Hung ◽  
Jui-Chang Tsai ◽  
Yu-Jung Liu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Continuous Monitoring ◽  
Mobile Genetic Element ◽  
Clonal Complex ◽  
Genetic Element ◽  
Novel Structure ◽  
Important Means ◽  
Sequence Types

ABSTRACTAntimicrobial resistance inStaphylococcus aureusis a major problem and the acquisition of resistance genes may occur by horizontal gene transfer (HGT). The transposon, an important means of HGT, is recognized as a mobile genetic element that can integrate in plasmids, replicate and transfer to other strains. We have previously reported a novel structure of theEnterococcus faecium-originated Tn1546-ermBelement inS. aureus. The emergence of the Tn1546-like element is an emerging problem that requires continuous monitoring. In the present study, we expand the examination of Tn1546-ermBelement toermB-positive methicillin-susceptibleS. aureus(MSSA) (n = 116) andermB-positive methicillin-resistantS. aureus(MRSA) (n = 253) during a 16-year period, from 2000 to 2015. PCR mapping showed that 10 MSSA and 10 MRSA carried the Tn1546-ermBelement. The 10 MSSA belonged to three sequence types (ST), ST7 (n = 6), ST5 (n = 3), and ST59 (n = 1), and the 10 MRSA belonged to two STs, ST188 (n = 8) and ST965 (n = 2). Since only clonal complex 5 (including ST5, ST85, ST231, and ST371) MRSA, ST8 MRSA and ST5 MSSA have been previously reported to carry Tn1546plasmids, this is the first report describing the presence of the Tn1546-ermBelement in ST7/5/59 MSSA and ST188/965 MRSA. Plasmid sequencing revealed that the Tn1546-ermBelement was harbored by five different mosaic plasmids. In addition to resistance genes, some plasmids also harbored toxin genes.

scholarly journals Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 625
Author(s):  
Elissavet Nikolaou ◽  
Alasdair T. M. Hubbard ◽  
João Botelho ◽  
Taylor A. M. Marschall ◽  
Daniela M. Ferreira ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Pneumococcal Infection ◽  
Mobile Genetic Elements ◽  
Genomic Islands ◽  
Mortality And Morbidity ◽  
Genetic Elements ◽  
Integrative And Conjugative Elements ◽  
Genetic Context

Pneumonia is the sixth largest cause of death in the UK. It is usually caused by Streptococcus pneumoniae, which healthy individuals can carry in their nose without symptoms of disease. Antimicrobial resistance further increases mortality and morbidity associated with pneumococcal infection, although few studies have analysed resistance in naturally circulating pneumococcal isolates in adult populations. Here, we report on the resistome and associated mobile genetic elements within circulating pneumococcus isolated from adult volunteers enrolled in the experimental human pneumococcal colonisation (EHPC) research program at the Liverpool School of Tropical Medicine, UK. Pneumococcal isolates collected from 30 healthy asymptomatic adults who had volunteered to take part in clinical research were screened for antibiotic susceptibility to erythromycin and tetracycline, and whole-genome sequenced. The genetic context of resistance to one or both antibiotics in four isolates was characterised bioinformatically, and any association of the resistance genes with mobile genetic elements was determined. Tetracycline and macrolide resistance genes [tet(M), erm(B), mef(A), msr(D)] were detected on known Tn916-like integrative and conjugative elements, namely Tn6002 and Tn2010, and tet(32) was found for the first time in S. pneumoniae located on a novel 50 kb genomic island. The widespread use of pneumococcal conjugate vaccines impacts on serotype prevalence and transmission within the community. It is therefore important to continue to monitor antimicrobial resistance (AMR) genes present in both vaccine types and non-vaccine types in response to contemporary antimicrobial therapies and characterise the genetic context of acquired resistance genes to continually optimise antibiotic therapies.

scholarly journals In Vitro Activity of Telithromycin against SpanishStreptococcus pneumoniae Isolates with Characterized Macrolide Resistance Mechanisms

2001 ◽  
Vol 45 (9) ◽  
pp. 2427-2431 ◽  
Author(s):  
Marı́a-Isabel Morosini ◽  
Rafael Cantón ◽  
Elena Loza ◽  
Marı́a-Cristina Negri ◽  
Juan-Carlos Galán ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Resistance Mechanisms ◽  
Macrolide Resistance ◽  
Vitro Activity ◽  
Penicillin G ◽  
In Vitro Activity ◽  
Resistance Phenotype ◽  
Erythromycin A

ABSTRACT The susceptibilities to telithromycin of 203 Streptococcus pneumoniae isolates prospectively collected during 1999 and 2000 from 14 different geographical areas in Spain were tested and compared with those to erythromycin A, clindamycin, quinupristin-dalfopristin, penicillin G, cefotaxime, and levofloxacin. Telithromycin was active against 98.9% of isolates (MICs, ≤0.5 μg/ml), with MICs at which 90% of isolates are inhibited being 0.06 μg/ml, irrespective of the resistance genotype. The corresponding values for erythromycin were 61.0% (MICs, ≤0.25 μg/ml) and >64 μg/ml. The erm(B) gene (macrolide-lincosamide-streptogramin B resistance phenotype) was detected in 36.4% (n = 74) of the isolates, which corresponded to 93.6% of erythromycin-intermediate and -resistant isolates, whereas the mef(A) gene (M phenotype [resistance to erythromycin and susceptibility to clindamycin and spiramycin without blunting]) was present in only 2.4% (n = 5) of the isolates. One of the latter isolates also carried erm(B). Interestingly, in one isolate for which the erythromycin MIC was 2 μg/ml, none of these resistance genes could be detected. Erythromycin MICs forS. pneumoniae erm(B)-positive isolates were higher (range, 0.5 to >64 μg/ml) than those for erm(B)- andmef(A)-negative isolates (range, 0.008 to 2 μg/ml). The corresponding values for telithromycin were lower for both groups, with ranges of 0.004 to 1 and 0.002 to 0.06 μg/ml, respectively. The erythromycin MIC was high for a large number oferm(B)-positive isolates, but the telithromycin MIC was low for these isolates. These results indicate the potential usefulness of telithromycin for the treatment of infections caused by erythromycin-susceptible and -resistant S. pneumoniaeisolates when macrolides are indicated.

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