scholarly journals Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal

2004 ◽  
Vol 48 (9) ◽  
pp. 3613-3617 ◽  
Author(s):  
Carla Novais ◽  
Teresa M. Coque ◽  
João Carlos Sousa ◽  
Fernando Baquero ◽  
Luisa Peixe
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Genetic Patterns ◽  
Single Clone ◽  
Vancomycin Resistant

ABSTRACT Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

scholarly journals Isolation of VanA-Type Vancomycin-Resistant Enterococcus Strains from Domestic Poultry Products with Enrichment by Incubation in Buffered Peptone Water at 42�C

2010 ◽  
Vol 76 (15) ◽  
pp. 5317-5320 ◽  
Author(s):  
Tetsuya Harada ◽  
Masashi Kanki ◽  
Takao Kawai ◽  
Masumi Taguchi ◽  
Tsutomu Asao ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Poultry Products ◽  
Domestic Poultry ◽  
Peptone Water ◽  
Vancomycin Resistant

ABSTRACT Eight VanA-type enterococcal strains were isolated from 8 of 171 domestic poultry products by using enrichment by incubation in buffered peptone water at 35�C and 42�C. The pulsed-field gel electrophoresis patterns of all six VanA-type Enterococcus faecalis isolates were nearly indistinguishable, indicating the presence of a specific clone in Japan.

scholarly journals Vancomycin-Resistant Enterococcus faecium: Catheter Colonization, esp Gene, and Decreased Susceptibility to Antibiotics in Biofilm

2005 ◽  
Vol 49 (12) ◽  
pp. 5046-5050 ◽  
Author(s):  
Issam I. Raad ◽  
Hend A. Hanna ◽  
Maha Boktour ◽  
Gassan Chaiban ◽  
Ray Y. Hachem ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Antibiotic Susceptibility ◽  
Pulsed Field Gel Electrophoresis ◽  
Molecular Characteristics ◽  
Vancomycin Resistant Enterococcus ◽  
Catheter Colonization ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Gastrointestinal Colonization

ABSTRACT To evaluate the molecular characteristics and antibiotic susceptibility in biofilm of vancomycin-resistant Enterococcus faecium (VREF) organisms that had caused catheter-related VREF bacteremia (VREF-CRB), we compared 22 isolates causing bacteremia obtained from patients with VREF-CRB with 30 isolates from control patients with gastrointestinal colonization by VREF. Using pulsed-field gel electrophoresis, we identified 17 unique strains among the 22 VREF-CRB isolates and 23 strains among the gastrointestinal isolates. The esp gene was detected in 53% (9 of 17) of the VREF-CRB and 61% (14 of 23) of the control strains (P = 0.6). VREF-CRB produced heavier biofilm colonization of silicone disks than did control organisms (P < 0.001). Daptomycin, minocycline, and quinupristin-dalfopristin were each independently more active than linezolid in reducing biofilm colonization by VREF-CRB (P < 0.01), with daptomycin being the most active, followed by minocycline. In conclusion, the esp gene in VREF is not associated with heavy biofilm colonization or catheter-related bacteremia. In biofilm, daptomycin and minocycline were the most active antibiotics against VREF, and linezolid was the least active.

Identification of Enterococcus faecalis in Different Clonal Lineages with the Pulsed-Field Gel Electrophoresis Method in Imam Khomeini Hospital, Ilam, Iran

2021 ◽  
Vol 14 (11) ◽  
Author(s):  
Farzad Mohamadi ◽  
Jalil Vand Yousefi ◽  
Naser Harzandi ◽  
Sobhan Ghafourian
Keyword(s):  
Enterococcus Faecalis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Disk Diffusion ◽  
Pulsed Field ◽  
Vana Gene ◽  
Electrophoresis Method ◽  
Typing Methods ◽  
Vancomycin Resistant ◽  
Tract Infections

Background: Due to the importance of identifying the source of infectious agents, different typing methods have been developed, among which the pulsed-field gel electrophoresis (PFGE) method is known as the gold standard for bacteria. Also, Enterococcus faecalis is classified as a nosocomial infection. Objectives: The current study aimed to identify the source of E. faecalis by the PFGE method. Methods: Bacteria were collected from all cases of urinary tract infections. Then, the identification process was performed. All isolates were evaluated for vancomycin resistance, and then PFGE was carried out. Results: The results of disk diffusion showed that 54% of the isolates showed resistance to vancomycin. Also, 4% of the isolates were intermediate, and 42% showed sensitivity to vancomycin. Afterwards, the PCR of the VanA gene was performed to confirm the results of disk diffusion. Thus, 48 out of 54 (88.8%) isolates had the VanA gene, and none of the four intermediate isolates had the VanA gene. Our results demonstrated that 54 isolates were vancomycin-resistant, and 50 different pulsotypes groups were identified. Conclusions: Our findings showed the isolates of E. faecalis were from different clonal lineages.

scholarly journals Vancomycin-Resistant Enterococcus faecium Strains Isolated from Community Wastewater from a Semiclosed Agri-Food System in Texas

2005 ◽  
Vol 49 (10) ◽  
pp. 4382-4385 ◽  
Author(s):  
T. L. Poole ◽  
M. E. Hume ◽  
L. D. Campbell ◽  
H. M. Scott ◽  
W. Q. Alali ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Food System ◽  
Pulsed Field Gel Electrophoresis ◽  
Vancomycin Resistant Enterococcus ◽  
Pulsed Field ◽  
Vancomycin Resistant ◽  
Fecal Waste

ABSTRACT Vancomycin-resistant Enterococcus faecium strains (VRE) were isolated from human wastewater but not swine fecal waste from a semiclosed agri-food system in Texas. Forty-nine VRE isolates possessed vanA, and one possessed vanB. Twenty-one pulsed-field gel electrophoresis types were identified and segregated into three groups. There was evidence of clonal dissemination among geographically separated sites.

scholarly journals Antimicrobial Resistance in Enterococci Isolated from Turkey Flocks Fed Virginiamycin

1998 ◽  
Vol 42 (3) ◽  
pp. 705-708 ◽  
Author(s):  
L. A. Welton ◽  
L. A. Thal ◽  
M. B. Perri ◽  
S. Donabedian ◽  
J. McMahon ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

ABSTRACT From 125 separate cloacal cultures from three turkey flocks fed virginiamycin, 104 Enterococcus faecium and 186Enterococcus faecalis isolates were obtained. As the turkeys aged, there was a higher percentage of quinupristin-dalfopristin-resistant E. faecium isolates, with isolates from the oldest flock being 100% resistant. There were no vancomycin-resistant enterococci. Results of pulsed-field gel electrophoresis (PFGE) indicated there were 11 PFGE types of E. faecalis and 7 PFGE types of E. faecium that were in more than one group of flock cultures.

Sign in / Sign up

Export Citation Format

Share Document