scholarly journals Potential of the Melanophore Pigment Response for Detection of Bacterial Toxicity

2010 ◽  
Vol 76 (24) ◽  
pp. 8243-8246 ◽  
Author(s):  
Stephanie R. Dukovcic ◽  
Janine R. Hutchison ◽  
Janine E. Trempy
Keyword(s):  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Aeromonas Salmonicida ◽  
Brown Pigment ◽  
Toxic Substances ◽  
Human Pathogen ◽  
Yersinia Ruckeri ◽  
Cell Type ◽  
Flavobacterium Psychrophilum ◽  
Bacterial Toxicity

ABSTRACT Chromatophore cells have been investigated as potential biodetectors for function-based detection of chemically and biologically toxic substances. Oncorhynchus tshawytscha (chinook salmon) melanophores, a chromatophore cell type containing brown pigment, rapidly detect the salmonid pathogens Aeromonas salmonicida, Yersinia ruckeri, and Flavobacterium psychrophilum and the human pathogen Bacillus cereus.

Genetic Evidence of Vertical Transmission and Cycling of Yersinia ruckeri in Hatchery-Origin Fall Chinook Salmon Oncorhynchus tshawytscha

2014 ◽  
Vol 6 (1) ◽  
pp. 44-54 ◽  
Author(s):  
Richard A. Glenn ◽  
Peter W. Taylor ◽  
Eric H. Pelton ◽  
Susan K. Gutenberger ◽  
Mark A. Ahrens ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Juvenile Fish ◽  
Kidney Tissue ◽  
Production Cycle ◽  
Yersinia Ruckeri ◽  
Nested Polymerase Chain Reaction ◽  
Severe Problem ◽  
Real Time Quantitative Pcr

Abstract Enteric redmouth disease, caused by the bacterium Yersinia ruckeri, can be a severe problem in the culture of salmonid fishes, and can persist as a chronic infection within hatchery facilities for an indefinite amount of time ranging from months to years. Using a nested polymerase chain reaction (nPCR) assay and a real-time quantitative PCR (qPCR) assay, the prevalence of Y. ruckeri deoxyribonucleic acid (DNA) was documented across a single production cycle of fall Chinook salmon Oncorhynchus tshawytscha reared at a hatchery. Ovarian fluid from spawning female adults, unfertilized eggs, eyed eggs, sac fry, and juvenile fish all tested positive for Y. ruckeri DNA using both nPCR and qPCR assays. Presence of Y. ruckeri DNA in ovarian fluids and unfertilized eggs indicates that vertical transmission of the pathogen from females to their progeny is possible. Juvenile fish had the highest level of Y. ruckeri DNA at a log10-transformed level of 4.3 DNA copies per gram of kidney tissue. The limited mortality observed was attributed to handling and hatchery procedures during the study period and indicates that the low levels of the pathogen's DNA can persist without severe detrimental effects over the hatchery rearing cycle.

scholarly journals Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR

2002 ◽  
Vol 68 (10) ◽  
pp. 5177-5180 ◽  
Author(s):  
A. del Cerro ◽  
I. Marquez ◽  
J. A. Guijarro
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Aeromonas Salmonicida ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Yersinia Ruckeri ◽  
Pcr Assay ◽  
Fish Pathogens ◽  
Flavobacterium Psychrophilum ◽  
Multiplex Pcr Assay

ABSTRACT A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.

Field Testing of Injected Vibrio anguillarum Bacterins in Pen-Reared Pacific Salmon

1976 ◽  
Vol 33 (6) ◽  
pp. 1291-1296 ◽  
Author(s):  
Ross Antipa
Keyword(s):  
Chinook Salmon ◽  
Intraperitoneal Injection ◽  
Oncorhynchus Tshawytscha ◽  
Coho Salmon ◽  
Pacific Salmon ◽  
Serum Antibody ◽  
Vibrio Anguillarum ◽  
Field Testing ◽  
Aeromonas Salmonicida ◽  
Control Group

Intraperitoneal injection of three types of Vibrio anguillarum bacterin (heat-killed, formalin-killed, and a combination of heat and formalin-killed) was tested in Chinook (Oncorhynchus tshawytscha) and coho salmon (O. kisutch) to assess the feasibility of preventing vibriosis by immunization in commercial aquaculture. A single injection of V. anguillarum bacterin gave substantial protection to chinook salmon for 6 mo postinoculation (32% mean mortality in all injected groups) in comparison to the control group (85% mortality). Chinook salmon showed the highest survival when treated with the heat-killed V. anguillarum bacterin. All groups of coho salmon had less than 7.0% mortality, and immunization was not beneficial to survival. Serum antibody titer of V. anguillarum agglutinins was increased by intraperitoneal injection. Vibrio anguillarum was the pathogen most frequently isolated from moribund chinook salmon; Aeromonas salmonicida was the predominant organism isolated from moribund coho salmon.

AN EPIZOOTIC IN CHINOOK SALMON (ONCORHYNCHUS TSHAWYTSCHA) CAUSED BY A SORBITOL-POSITIVE SEROVAR 2 STRAIN OF YERSINIA RUCKERI

1986 ◽  
Vol 22 (4) ◽  
pp. 488-492 ◽  
Author(s):  
Rocco C. Cipriano ◽  
W. B. Schill ◽  
Stephen W. Pyle ◽  
Rodney Horner
Keyword(s):  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Yersinia Ruckeri
Sign in / Sign up

Export Citation Format

Share Document