Population pharmacokinetics/pharmacodynamics modelling of enrofloxacin for the three major trout pathogens Aeromonas salmonicida, Flavobacterium psychrophilum and Yersinia ruckeri

ABSTRACT A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.

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Potential of the Melanophore Pigment Response for Detection of Bacterial Toxicity

Applied and Environmental Microbiology ◽

10.1128/aem.01241-10 ◽

2010 ◽

Vol 76 (24) ◽

pp. 8243-8246 ◽

Cited By ~ 3

Author(s):

Stephanie R. Dukovcic ◽

Janine R. Hutchison ◽

Janine E. Trempy

Keyword(s):

Chinook Salmon ◽

Oncorhynchus Tshawytscha ◽

Aeromonas Salmonicida ◽

Brown Pigment ◽

Toxic Substances ◽

Human Pathogen ◽

Yersinia Ruckeri ◽

Cell Type ◽

Flavobacterium Psychrophilum ◽

Bacterial Toxicity

ABSTRACT Chromatophore cells have been investigated as potential biodetectors for function-based detection of chemically and biologically toxic substances. Oncorhynchus tshawytscha (chinook salmon) melanophores, a chromatophore cell type containing brown pigment, rapidly detect the salmonid pathogens Aeromonas salmonicida, Yersinia ruckeri, and Flavobacterium psychrophilum and the human pathogen Bacillus cereus.

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Intra-ovum Infection in Salmonid Eggs Artificially Contaminated with Fish Pathogenic Bacteria: Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida

Fish Pathology ◽

10.3147/jsfp.47.49 ◽

2012 ◽

Vol 47 (2) ◽

pp. 49-55 ◽

Cited By ~ 6

Author(s):

Masakazu Kohara ◽

Hisae Kasai ◽

Mamoru Yoshimizu

Keyword(s):

Pathogenic Bacteria ◽

Aeromonas Salmonicida ◽

Renibacterium Salmoninarum ◽

Flavobacterium Psychrophilum ◽

Salmonid Eggs

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The production and methods of use of polyclonal antisera to the pathogenic organisms Aeromonas salmonicida, Yersinia ruckeri and Renibacterium salmoninarum

Journal of Fish Biology ◽

10.1111/j.1095-8649.1987.tb05317.x ◽

1987 ◽

Vol 31 (sa) ◽

pp. 225-226 ◽

Cited By ~ 6

Author(s):

A. M. Smith ◽

O. L. Goldring ◽

G. Dear

Keyword(s):

Aeromonas Salmonicida ◽

Yersinia Ruckeri ◽

Renibacterium Salmoninarum ◽

Polyclonal Antisera ◽

Pathogenic Organisms

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Denaturing gradient gel electrophoresis for nonlethal detection of Aeromonas salmonicida in salmonid mucus and its potential for other bacterial fish pathogens

Canadian Journal of Microbiology ◽

10.1139/w2012-024 ◽

2012 ◽

Vol 58 (5) ◽

pp. 563-571 ◽

Cited By ~ 2

Author(s):

Robert A. Quinn ◽

Roselynn M.W. Stevenson

Keyword(s):

Organ Culture ◽

Gel Electrophoresis ◽

Lake Trout ◽

Denaturing Gradient Gel Electrophoresis ◽

Coho Salmon ◽

Simultaneous Detection ◽

Aeromonas Salmonicida ◽

Band Pattern ◽

Yersinia Ruckeri ◽

Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA was used to nonlethally detect Aeromonas salmonicida and other bacteria in salmonid skin mucus. Mucus samples from wild spawning coho salmon ( Oncorhynchus kisutch ) with endemic A. salmonicida and from cultured lake trout ( Salvelinus namaycush ) were tested by PCR–DGGE and were compared with mucus culture on Coomassie brilliant blue agar and internal organ culture. PCR–DGGE gave a highly reproducible 4-band pattern for 9 strains of typical A. salmonicida, which was different from other Aeromonas spp. Aeromonas salmonicida presence in mucus was evident as a band that comigrated with the bottom band of the A. salmonicida 4-band pattern and was verified by sequencing. PCR–DGGE found 36 of 52 coho salmon positive for A. salmonicida, compared with 31 positive by mucus culture and 16 by organ culture. Numerous other bacteria were detected in salmonid mucus, including Pseudomonas spp., Shewanella putrefaciens , Aeromonas hydrophila and other aeromonads. However, Yersinia ruckeri was not detected in mucus from 27 lake trout, but 1 fish had a sorbitol-positive Y. ruckeri isolated from organ culture. Yersinia ruckeri seeded into a mucus sample suggested that PCR–DGGE detection of this bacterium from mucus was possible. PCR–DGGE allows nonlethal detection of A. salmonicida in mucus and differentiation of some Aeromonas spp. and has the potential to allow simultaneous detection of other pathogens present in fish mucus.

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Advances in Fisheries Bioengineering

Advances in Fisheries Bioengineering ◽

10.47886/9781934874028.ch6 ◽

2008 ◽

Keyword(s):

Water Quality ◽

Quality Parameters ◽

Aeromonas Salmonicida ◽

Fish Culture ◽

Water Filtration ◽

Culturable Bacteria ◽

Yersinia Ruckeri ◽

Creek Water ◽

Before And After ◽

The U.S

Abstract.—A water filtration and ozonation system was recently installed to treat creek water used to culture species of concern at the U.S. Fish and Wildlife Service’s Northeast Fishery Center, Lamar National Fish Hatchery (NFH). Past experience with fish culture indicates that the following bacterial pathogens are endemic to the creek water supply: Aeromonas salmonicida, Yersinia ruckeri, Flavobacterium columnaris, and Flavobacterium psychrophilia. Water samples were collected from sites located before and after filtration and ozonation and examined for culturable bacteria. Variable operation of the filtration/ozonation system was used to examine (1) the effect of microscreen filtration (i.e., using drum filters containing 37-μm sieve panels) on ozone inactivation of bacterial microorganisms, (2) the effect of dissolved ozone contact times on inactivation of bacterial microorganisms, and (3) the effect of water quality fluctuations on the dissolved ozone demand measured during the course of these tests. Inactivation exceeded 98% for all bacteria when ozone C*t values were about 1.0 and reached 100% at 21.3, regardless of water quality parameters or implementation of microscreen filtration. These results indicate that the use of ozonation to treat surface water supplies used for fish culture facilities will effectively inactivate the majority of bacteria entering the system and will likely serve to prevent introduction of bacteria that can be pathogenic to fish.

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