Cloning and Characterization of α-Methylacyl Coenzyme A Racemase from Gordonia polyisoprenivorans VH2

ABSTRACT The mcr gene of Gordonia polyisoprenivorans VH2 is not clustered with genes required for rubber degradation. Its disruption by insertion of a kanamycin resistance cassette impaired growth on methyl-branched isoprenoids but not on linear hydrocarbons. Intact mcr from this bacterium or from Nocardia farcinica IFM 10152 complemented the mutant. Reverse transcription analysis showed similar mcr VH2 expression results during cultivation with poly(cis-1,4-isoprene) and propionate. Additional genes coding for a putative cytochrome P450 monooxygenase and a short-chain dehydrogenase/reductase involved in β-oxidation and poly(cis-1,4-isoprene) degradation were also characterized.

Download Full-text

Characterization of P450 FcpC, the Enzyme Responsible for Bioconversion of Diosgenone to Isonuatigenone in Streptomyces virginiae IBL-14

Applied and Environmental Microbiology ◽

10.1128/aem.02606-08 ◽

2009 ◽

Vol 75 (12) ◽

pp. 4202-4205 ◽

Cited By ~ 11

Author(s):

Wei Wang ◽

Feng-Qing Wang ◽

Dong-Zhi Wei

Keyword(s):

Escherichia Coli ◽

Electron Transfer ◽

Cytochrome P450 ◽

Cytochrome P450 Monooxygenase ◽

P450 Monooxygenase ◽

Whole Cell ◽

Electron Transfer Chain ◽

Streptomyces Virginiae ◽

Transfer Chain

ABSTRACT A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

Download Full-text

Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca

Applied Microbiology and Biotechnology ◽

10.1007/s00253-010-2965-9 ◽

2010 ◽

Vol 89 (5) ◽

pp. 1475-1485 ◽

Cited By ~ 39

Author(s):

Anett Schallmey ◽

Gijs den Besten ◽

Ite G. P. Teune ◽

Roga F. Kembaren ◽

Dick B. Janssen

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 Monooxygenase ◽

Soil Bacterium ◽

Thermobifida Fusca ◽

P450 Monooxygenase

Download Full-text

Characterization of the cytochrome P450 monooxygenase genes (P450ome) from the carotenogenic yeast Xanthophyllomyces dendrorhous

BMC Genomics ◽

10.1186/s12864-017-3942-9 ◽

2017 ◽

Vol 18 (1) ◽

Cited By ~ 11

Author(s):

Pamela Córdova ◽

Ana-María Gonzalez ◽

David R. Nelson ◽

María-Soledad Gutiérrez ◽

Marcelo Baeza ◽

...

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 Monooxygenase ◽

Xanthophyllomyces Dendrorhous ◽

P450 Monooxygenase

Download Full-text

Cloning, expression, and characterization of a self-sufficient cytochrome P450 monooxygenase from Rhodococcus ruber DSM 44319

Applied Microbiology and Biotechnology ◽

10.1007/s00253-006-0355-0 ◽

2006 ◽

Vol 72 (5) ◽

pp. 876-882 ◽

Cited By ~ 40

Author(s):

Luo Liu ◽

Rolf D. Schmid ◽

Vlada B. Urlacher

Keyword(s):

Cytochrome P450 ◽

Cytochrome P450 Monooxygenase ◽

Rhodococcus Ruber ◽

P450 Monooxygenase

Download Full-text

Cloning and Characterization of the Biosynthetic Gene Cluster of 16-Membered Macrolide Antibiotic FD-891: Involvement of a Dual Functional Cytochrome P450 Monooxygenase Catalyzing Epoxidation and Hydroxylation

ChemBioChem ◽

10.1002/cbic.201000214 ◽

2010 ◽

Vol 11 (11) ◽

pp. 1574-1582 ◽

Cited By ~ 26

Author(s):

Fumitaka Kudo ◽

Atsushi Motegi ◽

Kazutoshi Mizoue ◽

Tadashi Eguchi

Keyword(s):

Cytochrome P450 ◽

Gene Cluster ◽

Macrolide Antibiotic ◽

Biosynthetic Gene Cluster ◽

Cytochrome P450 Monooxygenase ◽

Biosynthetic Gene ◽

P450 Monooxygenase ◽

Dual Functional

Download Full-text

Biocatalytic Conversion of Avermectin to 4"-Oxo-Avermectin: Characterization of Biocatalytically Active Bacterial Strains and of Cytochrome P450 Monooxygenase Enzymes and Their Genes

Applied and Environmental Microbiology ◽

10.1128/aem.71.11.6968-6976.2005 ◽

2005 ◽

Vol 71 (11) ◽

pp. 6968-6976 ◽

Cited By ~ 22

Author(s):

Volker Jungmann ◽

István Molnár ◽

Philip E. Hammer ◽

D. Steven Hill ◽

Ross Zirkle ◽

...

Keyword(s):

Escherichia Coli ◽

Cytochrome P450 ◽

Recombinant Proteins ◽

Oxidation Reaction ◽

Cytochrome P450 Monooxygenases ◽

Enzyme Kinetic ◽

Bacterial Strains ◽

P450 Monooxygenase ◽

P450 Monooxygenases

ABSTRACT 4"-Oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin. Seventeen biocatalytically active Streptomyces strains with the ability to oxidize avermectin to 4"-oxo-avermectin in a regioselective manner have been discovered in a screen of 3,334 microorganisms. The enzymes responsible for this oxidation reaction in these biocatalytically active strains were found to be cytochrome P450 monooxygenases (CYPs) and were termed Ema1 to Ema17. The genes for Ema1 to Ema17 have been cloned, sequenced, and compared to reveal a new subfamily of CYPs. Ema1 to Ema16 have been overexpressed in Escherichia coli and purified as His-tagged recombinant proteins, and their basic enzyme kinetic parameters have been determined.

Download Full-text