Use of Laser Microdissection for Phylogenetic Characterization of Polyphosphate-Accumulating Bacteria

ABSTRACT Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.

Download Full-text

Novel approach to Infrared spectroscopy and imaging for the routine characterization of microplastics in environmental samples

10.1021/scimeetings.0c05028 ◽

2020 ◽

Author(s):

Darren Robey

Keyword(s):

Infrared Spectroscopy ◽

Environmental Samples ◽

Novel Approach

Download Full-text

Novel approach to Infrared spectroscopy and imaging for the routine characterization of microplastics in environmental samples

10.1021/scimeetings.0c05027 ◽

2020 ◽

Author(s):

Darren Robey

Keyword(s):

Infrared Spectroscopy ◽

Environmental Samples ◽

Novel Approach

Download Full-text

Phylogenetic characterization of bromate-reducing microbial community enriched anaerobically from activated sludge

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2019.109630 ◽

2019 ◽

Vol 184 ◽

pp. 109630 ◽

Cited By ~ 1

Author(s):

Dan Wang ◽

Xunchao Cai ◽

Xinyue Lv ◽

Yicheng Wang ◽

Xue Gao ◽

...

Keyword(s):

Microbial Community ◽

Activated Sludge ◽

Phylogenetic Characterization

Download Full-text

Characterization of Pure Cultures Isolated from Sulfamethoxazole-acclimated Activated Sludge with Respect to Taxonomic Identification and Sulfamethoxazole Biodegradation Potential

Environmental Engineering and Activated Sludge Processes ◽

10.1201/b19902-12 ◽

2016 ◽

pp. 165-186

Keyword(s):

Activated Sludge ◽

Taxonomic Identification ◽

Pure Cultures ◽

Acclimated Activated Sludge

Download Full-text

Characterization of pure cultures isolated from sulfamethoxazole-acclimated activated sludge with respect to taxonomic identification and sulfamethoxazole biodegradation potential

BMC Microbiology ◽

10.1186/1471-2180-13-276 ◽

2013 ◽

Vol 13 (1) ◽

pp. 276 ◽

Cited By ~ 41

Author(s):

Bastian Herzog ◽

Hilde Lemmer ◽

Harald Horn ◽

Elisabeth Müller

Keyword(s):

Activated Sludge ◽

Taxonomic Identification ◽

Pure Cultures ◽

Acclimated Activated Sludge

Download Full-text

Approaches to assess the biodiversity of bacteria in natural habitats

Biochemical Society Transactions ◽

10.1042/bst0340169 ◽

2006 ◽

Vol 34 (1) ◽

pp. 169-173 ◽

Cited By ~ 3

Author(s):

C. Rösch ◽

S. Eilmus ◽

H. Bothe

Keyword(s):

Bacterial Community ◽

Environmental Samples ◽

Target Genes ◽

Pcr Amplification ◽

Restriction Enzymes ◽

Functional Genes ◽

Natural Habitats ◽

Uncultured Bacteria ◽

Computer Based

Any attempt to characterize a bacterial community and their functional genes coding for enzymes of the nitrogen cycle is faced with its extreme biodiversity. Novel techniques, based on PCR amplification of target genes in DNA from environmental samples, have been developed for characterizing both cultured and as yet uncultured bacteria in the last few years. Computer-based assignment tools have now been developed utilizing terminal restriction fragments obtained from digestions with multiple restriction enzymes. Such programs allow the gross characterization of bacterial life in any complex bacterial community with confidence.

Download Full-text