scholarly journals Burkholderia cepacia Genomovar III Is a Common Plant-Associated Bacterium

2001 ◽  
Vol 67 (2) ◽  
pp. 982-985 ◽  
Author(s):  
Jacques Balandreau ◽  
Veronique Viallard ◽  
Benoit Cournoyer ◽  
Tom Coenye ◽  
Severine Laevens ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Burkholderia Cepacia ◽  
Protein Electrophoresis ◽  
Cell Protein ◽  
Taxonomic Study ◽  
16S Ribosomal Dna ◽  
Whole Cell Protein ◽  
Phylogenetic Lineages ◽  
Common Plant ◽  
Ribosomal Dna Sequence

ABSTRACT A polyphasic taxonomic study involving DNA-DNA hybridization, whole-cell protein electrophoresis, and 16S ribosomal DNA sequence analysis revealed that a group of Burkholderia cepacia-like organisms isolated from the rhizosphere or tissues of maize, wheat, and lupine belong to B. cepacia genomovar III, a genomic species associated with “cepacia syndrome” in cystic fibrosis patients. The present study also revealed considerable protein electrophoretic heterogeneity within this species and demonstrated that the B. cepacia complex consists of two independent phylogenetic lineages.

The application of whole-cell protein electrophoresis for the classification and identification of basidiomycetous yeast species

1992 ◽  
Vol 61 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Marc Vancanneyt ◽  
Eddy Van Lerberge ◽  
Jean-Francois Berny ◽  
Gregoire L. Hennebert ◽  
Karel Kersters
Keyword(s):  
Yeast Species ◽  
Protein Electrophoresis ◽  
Basidiomycetous Yeast ◽  
Cell Protein ◽  
Whole Cell ◽  
Whole Cell Protein

Clusterization of halophilic and halotolerant eubacteria using whole-cell protein electrophoresis data

Microbiology ◽  
2000 ◽  
Vol 69 (5) ◽  
pp. 582-587 ◽  
Author(s):  
S. A. Bykova ◽  
I. S. Zvyagintseva ◽  
D. S. Akhlynin ◽  
S. S. Belyaev ◽  
V. F. Gal’chenko
Keyword(s):  
Protein Electrophoresis ◽  
Cell Protein ◽  
Whole Cell ◽  
Whole Cell Protein

A Study of Campylobacter in Sewage, Sewage Sludge and in River Water

1991 ◽  
Vol 24 (2) ◽  
pp. 117-120 ◽  
Author(s):  
W. Stelzer ◽  
J. Jacob
Keyword(s):  
Sewage Sludge ◽  
Treatment Plant ◽  
Electrophoretic Pattern ◽  
River System ◽  
Average Concentration ◽  
Selective Medium ◽  
Cell Protein ◽  
Oxidation Pond ◽  
River Waters ◽  
Whole Cell Protein

This study is aimed at determining the occurrence of campylobacters in waste water, sewage sludge and in a river system. The selective medium for the isolation of campylobacter consists of blood agar supplemented with the antibiotics vancomycin (10 µg/ml), cephalexin (15 µg/ml), trimethoprim (5 µg/ml), polymycin B (2.5 µg/ml) and rifampicin (5 µg/ml). Raw sewage samples contained about 103 Campylobacter/100 ml while the effluent showed an average concentration of 1.3 × 102/100 ml. Raw sewage from an oxidation pond treatment plant contained an average of 51 Campylobacter/100 ml while none were found in the effluent. No Campylobacter could be found in digested, conditioned sludge. The organism could be detected in 82.1% of river waters examined with the majority showing <10/100 ml. The presence of waterfowl and the faecal contamination from a poultry farm resulted in higher Campylobacter levels. About 50 % of the isolates typed as C. coli were really confirmed as C. jejuni by electrophoretic pattern (whole cell protein profiles).

Preliminary screening for toxin genes amongst stock cultures of Clostridium perfringens strains isolated from dogs and calves

2014 ◽  
Vol 4 (3) ◽  
pp. 127-132
Author(s):  
Egwari L. O. ◽  
Oghogho E. S. ◽  
Okwumabua O. E. ◽  
Oniha M. I.
Keyword(s):  
Clostridium Perfringens ◽  
Protein Profile ◽  
Animal Origin ◽  
Cell Protein ◽  
Toxin Gene ◽  
Whole Cell ◽  
University Of Wisconsin ◽  
Gene Type ◽  
Whole Cell Protein ◽  
Gene 4

The spectrum of Clostridium perfringens infections ranges from food toxinosis to myonecrosis. In the current study, whole cell protein and toxin gene types were profiled in 12 randomly selected C. perfringens veterinary stock cultures from the University of Wisconsin, Madison to determine epidemio-logical similarity, or diversity amongst strains of animal origin. Whole cell protein analysis was done by SDS-PAGE while toxin gene typing was achieved by extracting DNA by boiling, DNA concentration and purity was determined by spectrophotometer and nanodrop while separation was carried out by checking it on gel electrophoresis. Multiplex PCR was used to identify the toxigenic gene-type. C. perfringens B and C. perfringens EE with established profiles were used as control strains. Isolates typed included strains cp 296, 309, 12872 (from dogs) and 304, 305, 306, 341, 342, 10754, 12218-2, 12218-3, 12473 (from calves). All 12 strains possess the cpa gene, 4 strains have cpb2, 3 strains etx, 2 strains positive for cpe and 1 for cpb. None of the strains carries the itx gene. Two strains have only cpa gene however no strains has more than two toxin gene types, with cpa-cpb2 combination be-ing more frequent. C. perfringens 305 (etx and cpa) and 342 (cpe and cpa) shared the same protein profile but belong to different toxinotype. It is evi-dent that the cpa gene is a marker for all C. perfringens strains, and similarity in protein profile is not sine qua non for toxin gene type.

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