scholarly journals Involvement of Rhodocyclus-Related Organisms in Phosphorus Removal in Full-Scale Wastewater Treatment Plants

2002 ◽  
Vol 68 (6) ◽  
pp. 2763-2769 ◽  
Author(s):  
Julie L. Zilles ◽  
Jordan Peccia ◽  
Myeong-Woon Kim ◽  
Chun-Hsiung Hung ◽  
Daniel R. Noguera
Keyword(s):  
Wastewater Treatment ◽  
Dna Sequences ◽  
Phosphorus Removal ◽  
Bacterial Population ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Biological Phosphorus Removal ◽  
Total Bacterial Population ◽  
Polyphosphate Accumulating Organisms ◽  
University Of Cape Town

ABSTRACT The participation of organisms related to Rhodocyclus in full-scale enhanced biological phosphorus removal (EBPR) was investigated. By using fluorescent in situ hybridization techniques, the communities of Rhodocyclus-related organisms in two full-scale wastewater treatment plants were estimated to represent between 13 and 18% of the total bacterial population. However, the fractions of these communities that participated in polyphosphate accumulation depended on the type of treatment process evaluated. In a University of Cape Town EBPR process, the percentage of Rhodocyclus-related cells that contained polyphosphate was about 20% of the total bacterial population, but these cells represented as much as 73% of the polyphosphate-accumulating organisms (PAOs). In an aerated-anoxic EBPR process, Rhodocyclus-related PAOs were less numerous, accounting for 6% of the total bacterial population and 26% of the total PAO population. In addition, 16S ribosomal DNA sequences 99.9% similar to the sequences of Rhodocyclus-related organisms enriched in acetate-fed bench-scale EBPR reactors were recovered from both full-scale plants. These results confirmed the involvement of Rhodocyclus-related organisms in EBPR and demonstrated their importance in full-scale processes. In addition, the results revealed a significant correlation between the type of EBPR process and the PAO community.

Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal

2002 ◽  
Vol 46 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
J.L. Zilles ◽  
C.-H. Hung ◽  
D.R. Noguera
Keyword(s):  
Wastewater Treatment ◽  
Phosphorus Removal ◽  
Fluorescent In Situ Hybridization ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

The objective of this research was to assess the relevance of organisms related to Rhodocyclus in enhanced biological phosphorus removal in full-scale wastewater treatment plants. The presence of these organisms in full-scale plants was first confirmed by fluorescent in situ hybridization. To address which organisms were involved in phosphorus removal, a method was developed which selected polyphosphate-accumulating organisms from activated sludge samples by DAPI staining and flow cytometry. Sorted samples were characterized using fluorescent in situ hybridization. The results of these analyses confirmed the presence of organisms related to Rhodocyclus in full-scale wastewater treatment plants and supported the involvement of these organisms in enhanced biological phosphorus removal. However, a significant fraction of the polyphosphate-accumulating organisms were not related to Rhodocyclus.

Denitrification activity of polyphosphate accumulating organisms (PAOs) in full-scale wastewater treatment plants

2018 ◽  
Vol 78 (12) ◽  
pp. 2449-2458 ◽  
Author(s):  
Ana B. Lanham ◽  
Adrian Oehmen ◽  
Gilda Carvalho ◽  
Aaron M. Saunders ◽  
Per H. Nielsen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
In Situ Hybridisation ◽  
Full Scale ◽  
Type I ◽  
Fluorescence In Situ Hybridisation ◽  
Biological Phosphorus Removal ◽  
Aerobic Conditions ◽  
Polyphosphate Accumulating Organisms ◽  
P Removal

Abstract A comprehensive assessment of full-scale enhanced biological phosphorus removal (EBPR) plants (five plants, 19 independent tests) was undertaken to determine their effectiveness in terms of aerobic and anoxic P removal. By comparing parallel P uptake tests under only aerobic or under anoxic-aerobic conditions, results revealed that introducing an anoxic stage led to an overall P removal of on average 90% of the P removed under only aerobic conditions. This was achieved with negligible higher PHA and glycogen requirements, 30% lower overall oxygen consumption and with the simultaneous removal of nitrate, reducing up to an estimate of 70% of carbon requirements for simultaneous N and P removal. Varying fractions of denitrifying polyphosphate accumulating organisms (DPAOs), from an average of 25% to 84%, were found in different plants. No correlation was found between the DPAO fractions and EBPR configuration, season, or the concentration of any of the microbial groups measured via quantitative fluorescence in situ hybridisation. These included Type I and Type II Ca. Accumulibacter and glycogen accumulating organisms, suggesting that chemical batch tests are the best methodology for quantifying the potential of anoxic P removal in full-scale wastewater treatment plants.

Evaluation and design of full-scale wastewater treatment plants using biological process models

1995 ◽  
Vol 31 (2) ◽  
pp. 245-255 ◽  
Author(s):  
Glen T. Daigger ◽  
Daniel Nolasco
Keyword(s):  
Wastewater Treatment ◽  
Steady State ◽  
Phosphorus Removal ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Process Models ◽  
Plant Performance ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus ◽  
Steady State Performance

Results from application of the IAWQ Activated Sludge Model No. 1, either with or without the excess biological phosphorus removal model of Dold, to thirteen full-scale wastewater treatment plants are presented. For nitrogen removal applications the model is capable of accurately predicting full-scale plant performance and trends in performance, even using model default parameters. Additional work is needed to allow accurate predictions of the effect of reactor configuration and oxygen transfer systems on plant performance. The model of Dold accurately characterized the steady-state performance of biological nitrogen and phosphorus removal systems, but not their dynamic behavior. Detailed wastewater characterization is necessary to allow accurate prediction of the steady-state performance of biological phosphorus removal systems. Further work is necessary to demonstrate its applicability to dynamic applications.

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