scholarly journals Virus Succession Observed during an Emiliania huxleyi Bloom

2003 ◽  
Vol 69 (5) ◽  
pp. 2484-2490 ◽  
Author(s):  
Declan C. Schroeder ◽  
Joanne Oke ◽  
Matthew Hall ◽  
Gillian Malin ◽  
William H. Wilson
Keyword(s):  
Gel Electrophoresis ◽  
Protein Gene ◽  
Major Capsid Protein ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Emiliania Huxleyi ◽  
Capsid Protein Gene ◽  
Western Norway ◽  
Virus Genotypes ◽  
Gradient Gel Electrophoresis ◽  
Major Capsid Protein Gene

ABSTRACT Denaturing gradient gel electrophoresis was used as a molecular tool to determine the diversity and to monitor population dynamics of viruses that infect the globally important coccolithophorid Emiliania huxleyi. We exploited variations in the major capsid protein gene from E. huxleyi-specific viruses to monitor their genetic diversity during an E. huxleyi bloom in a mesocosm experiment off western Norway. We reveal that, despite the presence of several virus genotypes at the start of an E. huxleyi bloom, only a few virus genotypes eventually go on to kill the bloom.

Genetic and codon usage bias analyses of major capsid protein gene in Ranavirus

2020 ◽  
Vol 84 ◽  
pp. 104379
Author(s):  
Hai-feng Tian ◽  
Qiao-mu Hu ◽  
Han-bing Xiao ◽  
Ling-bing Zeng ◽  
Yan Meng ◽  
...  
Keyword(s):  
Codon Usage ◽  
Capsid Protein ◽  
Codon Usage Bias ◽  
Protein Gene ◽  
Major Capsid Protein ◽  
Capsid Protein Gene ◽  
Major Capsid Protein Gene

Nucleotide sequence and transcriptional mapping of the major capsid protein gene of pseudorabies virus

Virology ◽  
1991 ◽  
Vol 185 (1) ◽  
pp. 56-66 ◽  
Author(s):  
Shunji Yamada ◽  
Tadao Imada ◽  
Wakako Watanabe ◽  
Yoshikazu Honda ◽  
Sadayo Nakajima-Iijima ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Capsid Protein ◽  
Pseudorabies Virus ◽  
Protein Gene ◽  
Major Capsid Protein ◽  
Capsid Protein Gene ◽  
Transcriptional Mapping ◽  
Major Capsid Protein Gene

scholarly journals Molecular Dynamics of Emiliania huxleyi and Cooccurring Viruses during Two Separate Mesocosm Studies

2006 ◽  
Vol 73 (2) ◽  
pp. 554-562 ◽  
Author(s):  
Joaquín Martínez Martínez ◽  
Declan C. Schroeder ◽  
Aud Larsen ◽  
Gunnar Bratbak ◽  
William H. Wilson
Keyword(s):  
Calcium Binding ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Emiliania Huxleyi ◽  
Sequencing Analysis ◽  
Gene Encoding ◽  
Binding Motifs ◽  
Mesocosm Experiments ◽  
Virus Genotypes ◽  
Gradient Gel Electrophoresis ◽  
Genotypic Richness

ABSTRACT In this study we used denaturing gradient gel electrophoresis, sequencing analysis, and analytical flow cytometry to monitor the dynamics and genetic richness of Emiliania huxleyi isolates and cooccurring viruses during two mesocosm experiments in a Norwegian fjord in 2000 and 2003. We exploited variations in a gene encoding a protein with calcium-binding motifs (GPA) and in the major capsid protein (MCP) gene to assess allelic and genotypic richness within E. huxleyi and E. huxleyi-specific viruses (EhVs), respectively. To our knowledge, this is the first report that shows the effectiveness of the GPA gene for analysis of natural communities of E. huxleyi. Our results revealed the existence of a genetically rich, yet stable E. huxleyi and EhV community in the fjordic environment. Incredibly, the same virus and host genotypes dominated in separate studies conducted 3 years apart. Both E. huxleyi-dominated blooms contained the same six E. huxleyi alleles. In addition, despite the presence of at least six and four EhV genotypes at the start of the blooms in 2000 and 2003, respectively, the same two virus genotypes dominated the naturally occurring infections during the exponential and termination phases of the blooms in both years.

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